Screening of biosurfactant producers from petroleum hydrocarbon contaminated sources in cold marine environments

Cai, Qinhong; Zhang, Baiyu; Chen, Bing; Zhu, Zhiwen; Lin, Weiyun; Cao, Tong

doi:10.1016/j.marpolbul.2014.06.039

Cited by 96 publications

(41 citation statements)

References 31 publications

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“…On the other hand, after a careful observation of biochemical results and by considering the different responses to all the tests performed, we cannot exclude the affiliation to more than two species, thus explaining the differences at specie-specific level. Among isolates, Pseudomonas and Rhodococcus are well-known biosurfactant producers (Syldatk et al 1985;Desai and Banat 1997;Husain et al 1997;Coelho et al 2003;Maier 2003), but such capability was rarely reported for cold-adapted isolates (Yakimov et al 1999;Pini et al 2007;Cai et al 2014). Our Antarctic isolate Rhodococcus sp.…”

Section: Discussionmentioning

confidence: 99%

“…However, to date, among them only few marine cold-adapted biosurfactant producers have been characterized and they have been mainly isolated from seawater (Yakimov et al 1999;Pepi et al 2005;Pini et al 2007). Only recently, Cai et al (2014) reported on the isolation of biosurfactant-producing bacteria from petroleum hydrocarbon-contaminated sediment (and water) from North Atlantic, Canada.…”

Section: Introductionmentioning

confidence: 99%

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Biosurfactant production by Arctic and Antarctic bacteria growing on hydrocarbons

et al. 2015

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The risk of hydrocarbon contamination in marine polar areas is constantly increasing. Autochthonous bacteria, due to their ability to cope and survive under extreme environmental conditions, can play a fundamental role in the hydrocarbon degradation. The degradation process is often enhanced by the production of biosurfactant molecules. The present study reports for the first time on the isolation of biosurfactant-producing bacteria from Arctic and Antarctic shoreline sediments. A total of 199 psychrotolerant bacterial isolates were obtained from hydrocarbonamended (with crude or diesel oil) microcosms. A total of 18 isolates were selected for their ability to grow in the presence of crude oil and produce biosurfactants, as it was revealed by the production of good E 24 values (C50 %) and/or reduction in the surface tension (under 30 mN/m). The positive response of the isolates to both tests suggests a possible production of biosurfactants with emulsifying and interfacial activities. Biosurfactant-producing isolates were mainly affiliated to the genera Rhodococcus (14 isolates), followed by Pseudomonas (two isolates), Pseudoalteromonas (one isolate) and Idiomarina (one isolate). Thin-layer chromatography of biosurfactant crude extracts revealed that the majority of the selected isolates were able to produce glycolipidic surfactants. Our results enlarge the knowledge, which is still poor and fragmentary, on biosurfactant producers from polar areas and indicate marine polar sediments as a source of bacteria with potential applications in the remediation of hydrocarbon-contaminated cold environments.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biosurfactant production by Arctic and Antarctic bacteria growing on hydrocarbons

et al. 2015

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“…The produced bioemulsier was also tested in terms of its emulsication ability with different hydrocarbons and the stability of the formed emulsions under different pH, temperature and salinity conditions. 51 As a result, the Exiguobacterium sp. strain N4-1P was isolated along with 54 other isolates.…”

Section: Introductionmentioning

confidence: 99%

A novel bioemulsifier produced by Exiguobacterium sp. strain N4-1P isolated from petroleum hydrocarbon contaminated coastal sediment

et al. 2017

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In this study, Exiguobacterium N4-1P is reported as a bioemulsifier producer for the first time. The strain was found to be closely related to E. oxidotolerans strain T-2-2 T , E. antarcticum B7 T , and E. antarcticum B7 T with similarities of 99.47, 98.70 and 98.63%, respectively. Its phenotypic properties such as metabolic fingerprints, membrane composition, and cell morphology were determined. Different carbon sources were used for bioemulsifier production and diesel was confirmed to stimulate the yield effectively. The produced bioemulsifier is a complex mainly composed of lipopeptides with C16:0 (32.18%) and C18:0 (40.99%) as the primary fatty acids. The produced bioemulsifier could form emulsions effectively with diverse hydrocarbons. No foams were formed during the production and applications, which would facilitate its commercialization. The bioemulsifier was stable over a wide range of salinity (0-25%), pH (2-12), and temperature (below 50 C). Exiguobacterium N4-1P and the produced bioemulsifier fills knowledge gaps and has promising application potential in diverse fields, especially in environmental engineering.

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“…Por fim, para a produção dos meios foi necessário a utilização de uma solução salina com concentração de 10g/L, 5g/L, 2g/L e 0,2g/L de NaCl, Na2HPO4, KH2PO4 e MgSO4.7H2O, respectivamente, com fermentação de 56 horas a 15 ºC. Através da metodologia descrita por Cai et al (2014) determinou-se o índice de emulsificação, que se baseia na mistura do óleo mineral com a solução aquosa livre de células do caldo fermentativo, na proporção de 1:1. Com o auxílio de um vortex, a mistura foi fortemente agitada e após 24 horas de repouso analisada.…”

Section: Influência Da Fonte De Carbono E Nitrogênio Na Produção De Bunclassified

Influência Das Fontes De Carbono E Nitrogênio Na Produção De Biossurfactante Pela Rhodotorula Laryngis

Camargo¹,

Sousa²,

Pinheiro³

et al. 2017

Blucher Chemical Engineering Proceedings

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RESUMO -A produção de biosurfactantes, advém de microrganismos que são biodegradáveis, biocompatíveis e que possuem baixa toxicidade. Também observa-se que são estáveis em condições extremas como a variação do pH, temperatura e salinidade. O presente trabalho teve como objetivo analisar a influência das fontes de carbono e nitrogênio na produção do biossurfactante pela levedura Rhodotorula laryngis, codificada como L69 e justifica-se devido ao seu interesse ambiental, industrial e farmacêutico. Para analises foram realizados índice de emulsificação em óleo mineral e teste de tensão superficial. Como resultado para as fontes de nitrogênio e carbono: resíduos de abacaxi, resíduos de caju, peptona, extrato de levedura e nitrato de amônia. Após essa seleção de melhores fontes de nitrogênio e carbono já é possível estabelecer planejamos para otimização da produção de biossurfactante, utilizando resíduos agroindustriais como fonte de carbono. INTRODUÇÃOOs biossurfactantes já são utilizados em diversos processos industriais, como na influência em processos de biorremediação (através do metabolismo dos microrganismos promover a diminuição ou erradicação de determinados poluentes em locais de extrema dificuldade), tornando os poluentes biodisponíveis a sua biodegradação; na agricultura agindo no controle biológico; na indústria de alimentos como substituto aos emulsificantes sintéticos em maioneses, molhos, sorvetes (BANAT et al.,2000) como também na área de cosméticos já que suas características umectantes o deixa mais compatível com a pele o que gera um menor grau de irritação. (MAIER e SOBERÓN -CHAVEZ, 2000) Além disso, vale lembrar que os biossurfactantes advém de inúmeras possibilidades de formulações, as quais são principalmente influenciadas pelas fontes de carbono e de nitrogênio, entretanto a presença de fósforo, ferro, manganês e magnésio no meio de cultivo, podem maximizar a produção de biossurfactante. Outros pontos que potencializam o processo

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Screening of biosurfactant producers from petroleum hydrocarbon contaminated sources in cold marine environments

Cited by 96 publications

References 31 publications

Biosurfactant production by Arctic and Antarctic bacteria growing on hydrocarbons

Biosurfactant production by Arctic and Antarctic bacteria growing on hydrocarbons

A novel bioemulsifier produced by Exiguobacterium sp. strain N4-1P isolated from petroleum hydrocarbon contaminated coastal sediment

Influência Das Fontes De Carbono E Nitrogênio Na Produção De Biossurfactante Pela Rhodotorula Laryngis

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