Screening of Biopolymer Producing Bacteria Isolated from Some Brassica Plants

Sivakumar, Nallusamy; Al-Bahry, Saif N.; Al-Battashi, Huda

doi:10.1016/j.apcbee.2013.05.057

Cited by 9 publications

(6 citation statements)

References 9 publications

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“…MEOR is a biologically based technology, which mobilizes trapped oil by utilization of bioproducts through both in situ and ex situ processes. This technology has different mechanisms such as gas production, improving mobility ratio by oil viscosity reduction, biopolymer production, bioclogging, interfacial tension (IFT) reduction and wettability alteration (Armstrong and Wildenschild 2012a;Lazar et al 2007;Sarafzadeh et al 2013;Sivakumar et al 2013;Soudmandasli et al 2007;Zhao et al 2017). However, the last two mechanisms have great importance, since IFT and wettability control capillary forces in porous media.…”

Section: Introductionmentioning

confidence: 99%

Isolation and screening of Bacillus subtilis MJ01 for MEOR application: biosurfactant characterization, production optimization and wetting effect on carbonate surfaces

Veshareh

Azad

Deihimi

et al. 2018

J Petrol Explor Prod Technol

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The bacterial strain MJ01 was isolated from stock tank water of one of the Iranian south oil field production facilities. The 16S rRNA gene of isolate, MJ01, showed 99% similarity to Bacillus subtilis. The results revealed that biosurfactant produced by this strain was lipopeptide-like surfactin based on FTIR analysis. Critical micelle concentration of produced surfactin in distilled water was 0.06 g/l. Wettability study showed that at zero salinity surfactin can change original oil-wet state to water-wet state, but in seawater salinity it cannot modify the wettability significantly. To utilize this biosurfactant in ex situ MEOR process, economical and reservoir engineering technical parameters were considered to introduce a new optimization strategy using the response surface methodology. Comparing the result of this optimization strategy with the previous optimization research works was shown that significant save in use of nutrients is possible by using this medium. Furthermore, using this method leads to less formation damage due to the incompatibility of injecting fluid and formation brine, and less formation damage due to the bioplugging.

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Section: Introductionmentioning

confidence: 99%

Isolation and screening of Bacillus subtilis MJ01 for MEOR application: biosurfactant characterization, production optimization and wetting effect on carbonate surfaces

Veshareh

Azad

Deihimi

et al. 2018

J Petrol Explor Prod Technol

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“…On the other hand, a viscosity of about 1.0153 mPa was obtained by B. paraconglomeratum PHB. Sustainable viscosity values would arise under optimized conditions [ 15 ]. The polymers made from octanoic, hexanoic, nonanoic, and biodiesel fatty acids displayed reduced complex rheology as angular frequency increased.…”

Section: Discussionmentioning

confidence: 99%

“…The viscosity of biopolymers is regulated by several parameters, including molecular weight, concentration, temperature, shear rate and structural characteristics. The viscosity of PHB fermented broths and extracts can be determined by the Lovis method [ 15 ]. Capillary rheometry, thermal analysis and tensile tests were used to evaluate the strengths of PHB and biodegradable blends [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Physicochemical characterization of polyhydroxybutyrate (PHB) produced by the rare halophile Brachybacterium paraconglomeratum MTCC 13074

Mandragutti,

Jarso,

Godi

et al. 2024

Microb Cell Fact

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Background Polyhydroxybutyrate is a biopolymer produced by bacteria and archaea under nitrogen-limiting conditions. PHB is an essential polymer in the bioplastic sector because of its biodegradability, eco-friendliness, and adaptability. The characterization of PHB is a multifaceted process for studying the structure and its properties. This entire aspect can assure the long-term viability and performance attributes of the PHB. The characteristics of PHB extracted from the halophile Brachybacterium paraconglomeratum were investigated with the objective of making films for application in healthcare. Results This was the first characterization study on PHB produced by a rare halophile, Brachybacterium paraconglomeratum (MTCC 13074). In this study, the strain produced 2.72 g/l of PHB for.5.1 g/l of biomass under optimal conditions. Methods are described for the determination of the physicochemical properties of PHB. The prominent functional groups CH3 and C = O were observed by FT-IR and the actual chemical structure of the PHB was deduced by NMR. GCMS detects the confirmation of four methyl ester derivatives of the extracted PHB in the sample. Mass spectrometry revealed the molecular weight of methyl 3-hydroxybutyric acid (3HB) present in the extract. The air-dried PHB films were exposed to TGA, DSC and a universal testing machine to determine the thermal profile and mechanical stability. Additionally, the essential property of biopolymers like viscosity was also assessed for the extracted PHB. Conclusions The current study demonstrated the consistency and quality of B. paraconglomeratum PHB. Therefore, Brachybacterium sps are also a considerable source of PHB with desired characteristics for industrial production.

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“…An increase in viscosity indicates the increased production of biopolymers. However, better viscosity values appear at optimized conditions due to high yields of PHA [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Selective isolation and genomic characterization of biopolymer producer—a novel feature of halophile Brachybacterium paraconglomeratum MTCC 13074

Mandragutti

Gódi

2023

Journal of Genetic Engineering and Biotechnology

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Background Biopolymers like polyhydroxyalkanoates (PHA) are the best natural macromolecules to use as alternative to the synthetic polymers. Many prokaryotes accumulate PHA as cytoplasmic intracellular granules and their accumulation is triggered by starving conditions. The PHAs are ecofriendly and used to create biodegradable plastics. The microbial synthesized PHA had acquired global importance in industrial and biomedical sectors. Results Ten different bacterial strains were isolated for the screening of PHA producers from the estuarine region of the Bay of Bengal, Suryalanka in Bapatla. A yellowish slimy circular colony known as M4 is actively growing on selective minimal media and was screened for polymeric granules in its cytoplasm using Sudan Black B and confirmed with the fluorescent dye Nile blue A. All of the isolates were biochemically tested and isolate M4 is the most capable of growing at high NaCl concentrations (3.2 percent) and tests positive for catalase, methyl red. The M4 strain revealed clear hydrolysis of gelatin, starch, and casein. The 16S rRNA sequencing revealed that M4 is 99.72% of identity to Brachybacterium paraconglomeratum LMG 19861(T) in BLAST and the obtained strain was assigned with accession no. MTCC 13074 and deposited in NCBI with accession no. MW899045. The chief cellular fatty acids found in M4 were C14:0, C15:0, C16:0, C18:1cis-9, C18:0, iso-C15: 0, iso-C14: 0, anteiso-C17: 0 and C18:1-7. Crotonic acid formation from M4-PHB extract was detected at 235nm in a UV spectrophotometer. Methanolysis was done, and derivatives of polyhydroxybutyric acid (PHB) in the extract were analyzed using GC-MS. Increasing viscosity was seen in the extracts which confirms the presence of polymer in the extracts. Thermogravimetric analysis was studied to determine the thermal profile of the PHB in the extract of M4. Conclusion In the study, the selective screening and extraction of ecofriendly PHB from M4 strain was highlighted. Brachybacterium paraconglomeratum is a novel strain showed its uniqueness by producing few monomeric derivatives of PHB. The strain was reporting for the first time as PHA producer. B. paraconglomeratum has promising characteristics according to its metabolic profile. In addition, this study also helps to understand the diversity of bacteria isolated from marine sources.

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Screening of Biopolymer Producing Bacteria Isolated from Some Brassica Plants

Cited by 9 publications

References 9 publications

Isolation and screening of Bacillus subtilis MJ01 for MEOR application: biosurfactant characterization, production optimization and wetting effect on carbonate surfaces

Isolation and screening of Bacillus subtilis MJ01 for MEOR application: biosurfactant characterization, production optimization and wetting effect on carbonate surfaces

Physicochemical characterization of polyhydroxybutyrate (PHB) produced by the rare halophile Brachybacterium paraconglomeratum MTCC 13074

Selective isolation and genomic characterization of biopolymer producer—a novel feature of halophile Brachybacterium paraconglomeratum MTCC 13074

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