Screening of Biomarkers Involved in Idiopathic Pulmonary Fibrosis and Regulation of Upstream miRNAs

Gao, Li; Li, Peiying; Tian, Hongjun; Wu, Min; Yang, Jingping; Xu, Ximing

doi:10.1016/j.amjms.2021.06.027

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…As we know, the keloid is associated with sex hormone levels, which suggested that expression levels of hsa_circ_0072688 might be regulated by sex hormones. Among miRNAs in the hsa_circ_0072688-centered ceRNA networks, dysregulation of the-miR-146a-5p[38], has-miR-146b-5p [39], hsa-miR-373-3p [40], and hsa-miR-93-5p [41] were reported in organ brosis. KEGG and GO enrichment analysis results demonstrated that hsa_circ_0072688 is related to the extracellular matrix, cell adhesion, cell apoptosis, and stress ber, and is involved in Hippo, Wnt, p53, and SMAD signal transduction.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of Differentially Expressed Circular RNAs in Keloid Dermal Tissues

Liu

Zhang

Huang

et al. 2022

Preprint

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Background Keloid is a dermal fibroproliferative disease with various etiologies and unclear pathogenesis. Recent studies have revealed that circular RNAs (circRNAs) exerted regulatory functions through a competing endogenous RNA (ceRNA) pathway in keloid progression. However, the expression profiles of circRNAs in keloid dermal tissues (KDTs) remain unknown. This study aimed to identify differentially expressed circRNAs (DECs) and genes (DEGs) in KDTs, as well as to investigate the potential biological functionsof circRNAs based on the circRNA-miRNA-mRNA ceRNA network. Results Through high-throughput RNA sequencing (RNA-seq), we revealed 3467 DEGs (865 up- and 2602 down-regulated) and 330 DECs (162 up- and 168 down-regulated) in KDTs. To reveal the functions of DECs preliminarily, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed for the host genes. Further, the up- and down-regulated DECs-miRNAs-DEGs regulatory networks were constructed, respectively. The functional prediction for the target genes showed that the up-regulated ceRNA network was associated with extracellular matrix and multiple cellular functions. The down-regulated ceRNA network was enriched in cell-cell junction and other biological processes. Cytoscape was used to visualize each network's protein-protein interaction (PPI) network and identify hub genes. By quantitative Real-Time PCR (qRT-PCR), hsa_circ_0060927, hsa_circ_0071410, hsa_circ_0058092, hsa_circ_0002874, hsa_circ_0004682, hsa_circ_0072688, hsa_circ_0006401, and hsa_circ_0055954 were identified significantly up-regulated in KDTs. Within, hsa_circ_0072688, which was up-regulated both in KDTs and keloid dermal fibroblasts (KDFs), and located in the cytoplasm, might be a key circRNA and affect the progression of keloid by impacting extracellular matrix, cell adhesion, and cell apoptosis, etc. Conclusion This study not only filled a gap in the circRNA library of KDTs but also laid a foundation for probing the biological function of DECs in keloids. Hsa_circ_0072688 was thought to be a key circRNA and more experimental support is needed.

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Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis of Differentially Expressed Circular RNAs in Keloid Dermal Tissues

Liu

Zhang

Huang

et al. 2022

Preprint

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“…A recent meta-analysis using miRNA and gene expression profiles downloaded from the Gene Expression Omnibus database identified differentially expressed genes and miRNAs between an idiopathic pulmonary fibrosis (IPF) group and a normal group, and CLDN18 was identified as a potential IPF biomarker. However, the specific roles of these genes and miRNAs in IPF need further investigation [ 49 ]. Another study to analyze the expression, genetic alterations, and prognostic role of CLDN18 using public data from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), and Human Protein Atlas (HPA) databases demonstrated that the expression of CLDN18 was restricted to the lung and stomach in normal tissues and was significantly downregulated in GC but was ectopically overexpressed in some other cancer types.…”

Section: Introductionmentioning

confidence: 99%

Claudin18.2 is a novel molecular biomarker for tumor-targeted immunotherapy

Cao

Xing

et al. 2022

Biomark Res

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The claudin18.2 (CLDN18.2) protein, an isoform of claudin18, a member of the tight junction protein family, is a highly selective biomarker with limited expression in normal tissues and often abnormal expression during the occurrence and development of various primary malignant tumors, such as gastric cancer/gastroesophageal junction (GC/GEJ) cancer, breast cancer, colon cancer, liver cancer, head and neck cancer, bronchial cancer and non-small-cell lung cancer. CLDN18.2 participates in the proliferation, differentiation and migration of tumor cells. Recent studies have identified CLDN18.2 expression as a potential specific marker for the diagnosis and treatment of these tumors. With its specific expression pattern, CLDN18.2 has become a unique molecule for targeted therapy in different cancers, especially in GC; for example, agents such as zolbetuximab (claudiximab, IMAB362), a monoclonal antibody (mAb) against CLDN18.2, have been developed. In this review, we outline recent advances in the development of immunotherapy strategies targeting CLDN18.2, including monoclonal antibodies (mAbs), bispecific antibodies (BsAbs), chimeric antigen receptor T (CAR-T) cells redirected to target CLDN18.2, and antibody–drug conjugates (ADCs).

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Mir-155-5p targets TP53INP1 to promote proliferative phenotype in hypersensitivity pneumonitis lung fibroblasts

Espina-Ordoñez,

Balderas-Martínez,

Torres-Machorro

et al. 2024

Non-coding RNA Research

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Screening of Biomarkers Involved in Idiopathic Pulmonary Fibrosis and Regulation of Upstream miRNAs

Cited by 3 publications

References 32 publications

Comprehensive Analysis of Differentially Expressed Circular RNAs in Keloid Dermal Tissues

Comprehensive Analysis of Differentially Expressed Circular RNAs in Keloid Dermal Tissues

Claudin18.2 is a novel molecular biomarker for tumor-targeted immunotherapy

Mir-155-5p targets TP53INP1 to promote proliferative phenotype in hypersensitivity pneumonitis lung fibroblasts

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