Screening of barley germplasm for leaf blight (Bipolaris sorokiniana) resistance

Abstract

Spot blotch or leaf spot, caused by Bipolaris sorokiniana (earlierHelminthosporium sativum) is the most common form of leaf blights of barley which appears in warmer and humid weather. The genetic studies for leaf blight in barley have concluded complex inheritance of leaf blight resistance at adult plant stage. Current Indian barley varieties are mostly susceptible to leaf blight under high input conditions and attempts have been made to introduce resistance for this fungal disease in Indian barley. In the present study, a set of 85 barley germplasm accessions under epiphytotic conditions were screened for leaf blight disease occurrence. Of these lines, 68 genotypes were found resistant, 7 were moderately resistant, 7 were moderately susceptible and 3 were found susceptible. The genotypes grouped as resistant were able to keep the disease level on the plant at very low level may be useful in developing mapping populations for QTL studies. The molecular markers reported for QTL regions of leaf blight resistance were used to screen these lines to validate their applicability in molecular studies for leaf blight in Indian conditions. Three molecular markers (Ebmac 705, Bmag 606 and Bmag 225) located on 3H genomic region of barley were found associated with leaf blight resistance during molecular screening. This suggests that the genomic region at 3H chromosome is significantly involved in inheritance of leaf blight resistance in Indian barley. This study may prove useful in selection of plant material and marker assisted selection for incorporating leaf blight resistance in Indian barley. Bilgic, H., Steffenson, B. J. and Hayes, P . M. (2005). Comprehensive genetic analysis reveals differential expression of seedling and adult plant resistance to spot blotch in populations of barley. Theor. Appl. Genet., 111:1238-1250. Bilgic, H., Steffenson, B. J., and Hayes, P . M. (2006). Molecular mapping of loci conferring resistance to different pathotypes of the spot blotch pathogen in barley. Phytopathology, 96:699-708.

Evaluation of Barley Genotypes against Spot Blotch Disease in Inner Tarai Region of Nepal

“…Genotypes grouped as resistant had very low level of disease on plants with very small spots which were not spreading further like the susceptible genotypes. Concurrent with the present findings, it has been also reported by Jain et al (2014) who evaluated 85 barley germplasm under epiphytotic conditions and none germplasms were found immune, 68 genotypes were found resistant, 7 were moderately resistant, 7 were moderately susceptible and 3 were found susceptible. Saadi et al (2002) screened barley cultivars and found Beecher, Jimmah 59, Jimmah 54, Omani, K65 and Cooley were resistant against spot blotch.…”

Mechanisms formation of systemic acquired resistance in spring barley plants (<i>Hordeum vulgare</i> L.) during infection by the pathway of dark brown spot

“…Вредоносный потенциал гриба снижает урожайность зерновых культур (потери урожая зерна составляют от 38 до 87 %) [25]. Ситуация осложняется отсутствием устойчивых к данному заболеванию форм пшеницы и ячменя [26]. Исключение составляют самоклональные линии этих культур, которые способны в условиях относительно жесткого инфекционного фона сохранять урожайность.…”