Screening of bacterial endophytes as potential biocontrol agents against soybean diseases

Lopes, Karla Bianca de Almeida; Carpentieri-Pípolo, Valéria; Fira, Djordje; Balatti, Pedro Alberto; López, Silvina Marianela Yanil; Oro, Thiago Henrique; Pagliosa, Eduardo Stefani; Degrassi, Giuliano

doi:10.1111/jam.14041

Cited by 50 publications

(25 citation statements)

References 55 publications

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“…and Burkholderia spp. significantly inhibited the in vitro growth of the fungal pathogens Sclerotinia sclerotiorum , Phomopsis sojae and/or Rhizoctonia solani (de Almeida Lopes et al ). Endophytic fungi Epicoccum nigrum and Trichoderma atroviride reduced infection of potato plants by Rhizoctonia solani (Lahlali and Hijri ).…”

Section: Introductionmentioning

confidence: 99%

Apple endophyte community is shaped by tissue type, cultivar and site and has members with biocontrol potential againstNeonectria ditissima

2020

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Aims This research aimed to identify factors influencing endophyte community structure in apple shoots and the bioactivity of cultured representatives against the fungal pathogen Neonectria ditissima. Methods and Results The endophyte community in leaves and stems of the apple cultivars ‘Royal Gala’ and ‘Braeburn’ were analysed by a cultivation‐independent method (PCR‐DGGE) which showed that tissue type, cultivar and site were determinant factors, with the endophyte taxa in ‘Royal Gala’ more variable than that in ‘Braeburn’, with leaf endophyte communities typically differing from stems in both cultivars. Seasonal (spring vs autumn) and regional (Nelson vs Hawke’s Bay) variations were not obvious in woody stems. A collection of 783 bacterial and 87 fungal endophytes were recovered from leaves and stems of ‘Royal Gala’, ‘Braeburn’, ‘Scilate’ and/or ‘Scifresh’ from Nelson (nine sites) and Hawke’s Bay (five sites) in spring and from Nelson (three sites) in autumn. A dual culture plating assay was used to test their ability to inhibit the mycelial growth of N. ditissima. Thirteen bacterial (mean of percent inhibition ≥20%) and 17 fungal isolates were antagonistic towards N. ditissima. These isolates belonged to the bacterial genera Bacillus and Pseudomonas, and fungal genera Chaetomium, Epicoccum, Biscogniauxia, Penicillium, Diaporthe, Phlyctema and two unidentified fungal isolates. Conclusions Endophyte communities in apple shoots were determined by tissue type, cultivar and site. Endophytic bacterial and fungal isolates inhibiting N. ditissima growth in vitro were found. Significance and Impact of the Study These results provided new evidence of factors influencing apple endophyte community in New Zealand. Endophytes with potential to reduce N. ditissima infection were identified, with the potential to be developed into a biocontrol strategy for European canker.

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Section: Introductionmentioning

confidence: 99%

Apple endophyte community is shaped by tissue type, cultivar and site and has members with biocontrol potential againstNeonectria ditissima

2020

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“…Interestingly, de Almeida Lopes et al [121] isolated three endophytic strains of Bcc from soybean plants. e cultivation in nutrient broth produced bioactive lipopeptides, extracted by different methods (methanol, ethyl acetate, and ammonium sulfate precipitation), and qualitatively tested regarding their capacity to inhibit fungal (S. sclerotiorum, P. sojae, and R. solani) and bacterial (X. axonopodis pv.…”

Section: Discussionmentioning

confidence: 99%

Discovery of a Novel Lineage Burkholderia cepacia ST 1870 Endophytically Isolated from Medicinal Polygala paniculata Which Shows Potent In Vitro Antileishmanial and Antimicrobial Effects

Cruz

Paula

Nogueira

et al. 2021

International Journal of Microbiology

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In this study, we report the isolation and identification of an endophytic strain of Burkholderia cepacia (COPS strain) associated with Polygala paniculata roots. Polygala plants are rich sources of promising microbiomes, of which the literature reports several pharmacological effects, such as trypanocidal, antinociceptive, anesthetic, anxiolytics, and anticonvulsant activities. B. cepacia COPS belongs to a new sequence type (ST 1870) and harbors a genome estimated in 8.3 Mbp which exhibits the aminoglycosides and beta-lactams resistance genes aph(3′)-IIa and blaTEM-116, respectively. Analysis performed using MLST, average nucleotide identity, and digital DNA-DNA hybridization support its species-level identification and reveals its novel housekeeping genes alleles gyrB, lepA, and phaC. The root endophyte B. cepacia COPS drew our attention from a group of 14 bacterial isolates during the primary screening for being potentially active against Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Micrococcus luteus ATCC 9341, Escherichia coli ATCC 25922, and Candida albicans ATCC 10231 and exhibited the broad-spectrum activity against phytopathogenic fungi. In addition, COPS strain showed production of protease, lipase, and esterase in solid media, and its natural product extract showed potent inhibition against fungal plant pathogens, such as Moniliophthora perniciosa, whose antagonism index (89.32%) exceeded the positive control (74.17%), whereas Sclerotinia sclerotiorum and Ceratocystis paradoxa showed high percentages of inhibition (85.53% and 82.69%, respectively). COPS crude extract also significantly inhibited S. epidermidis ATCC 35984, E. faecium ATCC 700221 (MIC values of 32 μg/mL for both), E. faecalis ATCC 29212 (64 μg/mL), and S. aureus ATCC 25923 (128 μg/mL). We observed moderate antagonistic activity against A. baumannii ATCC 19606 and E. coli ATCC 25922 (both at 512 μg/mL), as well as potent cytotoxic effects on Leishmania infantum and Leishmania major promastigote forms with 78.25% and 57.30% inhibition. In conclusion, this study presents for the first time the isolation of an endophytic B. cepacia strain associated with P. paniculata and enough evidence that these plants may be considered a rich source of microbes for the fight against neglected diseases.

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“…Microbiomes of the major agricultural crops including Cotton ( Gossypium hirsutum L.), rice ( Oryza sativa L.) and soybean ( Glycine max (L.) Merr.) have been characterized (Edwards et al 2015; Liu et al 2019; Longley et al 2020; Qiao et al 2017; Roman-Reyna et al 2020; Ullah et al 2019), and beneficial endophytes have been detected (Bertani et al 2016; de Almeida Lopes et al 2018; Zhou et al 2018). Plant-associated microorganisms are increasingly used for biotechnological applications, including biological control of plant pathogens, plant growth promotion, or isolation of active compounds (Ryan et al 2008; Glick 2012; Bouizgarne 2013; Dey et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Curtobacterium glycinis sp. nov. from Glycine max, Curtobacterium gossypii sp. nov. from Gossypium hirsutum and Curtobacterium oryzae sp. nov. from Oryza sativa, three new Curtobacterium species and endophytes from agricultural crops

Seaton

Lemaire

Inderbitzin

et al. 2021

Preprint

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Screening of bacterial endophytes as potential biocontrol agents against soybean diseases

Cited by 50 publications

References 55 publications

Apple endophyte community is shaped by tissue type, cultivar and site and has members with biocontrol potential againstNeonectria ditissima

Apple endophyte community is shaped by tissue type, cultivar and site and has members with biocontrol potential againstNeonectria ditissima

Discovery of a Novel Lineage Burkholderia cepacia ST 1870 Endophytically Isolated from Medicinal Polygala paniculata Which Shows Potent In Vitro Antileishmanial and Antimicrobial Effects

Curtobacterium glycinis sp. nov. from Glycine max, Curtobacterium gossypii sp. nov. from Gossypium hirsutum and Curtobacterium oryzae sp. nov. from Oryza sativa, three new Curtobacterium species and endophytes from agricultural crops

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