Screening of Bacteria Isolated from Refinery Sludge of Assam for Hydrocarbonoclastic Activities

Abstract

Oily sludge generated by petroleum industries results in serious hazards to the environment due to its toxic effects. Microorganisms can survive in the harsh environment utilizing the pollutants as their sole source of carbon and energy. The present study was carried out to isolate and characterize the bacterial population present in the refinery wastes collected from Bongaigaon Refinery, Assam, India. The diversity of bacteria was significantly correlated (p< 0.05) with the physicochemical characteristics and level of hydrocarbon content of the studied samples. A total of 36 bacterial strains were isolated from the oily sludge sample. Two potent hydrocarbonoclastic bacteria were screened out based on their ability of degradation of petroleum hydrocarbon. The potent bacterial isolates also produced biosurfactants. The potent strains were identified as Pseudomonas sp. and Bacillus sp. Gravimetric estimation of hydrocarbon degradation and FTIR analysis revealed that these potent bacterial strains could remove different constituents of petroleum hydrocarbon. The indigenous bacterial isolates either alone or in consortium may be applied for degrading different fractions of hydrocarbon contaminants of the oily sludge to remediate the sludge polluted soil environment.

A Review on Rhizosphere Microbiota of Tea Plant (Camellia sinensis L): Recent Insights and Future Perspectives

“…They found that Azotobacter, Burkholderia, Enterobacter, and Rhizobium improved the growth of tea plants under salt stress. 112 These results highlight that tea plants benefit from their associated microbiomes to resist salt stress.…”

“…In a separate study, Huidrom et al evaluated the ability of primary bacteria isolated from tea garden soil to withstand salt stress. They found that Azotobacter , Burkholderia , Enterobacter , and Rhizobium improved the growth of tea plants under salt stress . These results highlight that tea plants benefit from their associated microbiomes to resist salt stress.…”

Pengaruh Fenantren terhadap Aktivitas Enzim Katabolik Pseudomonas Putida TI (8)

“…Enzim ini dibutuhkan oleh P. putida TI (8) sebagai katalis reaksi oksidasi hidrokarbon untuk mendegradasi fenantren (Kanaly & Harayama, 2000). Adanya enzim ini menjadikan bakteri mampu merubah hidrokarbon menjadi senyawa lebih kecil sehingga dapat dijadikan sebagai sumber makanan oleh bakteri (Devi & Jha, 2020). Bakteri ini dapat merubah dan memecah fenantren menjadi senyawa golongan catechol yang digunakan oleh bakteri untuk proses metabolisme asam sitrat (Hamzah dkk., 2011).…”