Screening of Bacillus spp. bacterial endophytes for protease production, and application in feather degradation and bio-detergent additive

Elaine Mankge, Malese; Penistacia Maela, Mehabo; Mark Abrahams, Adrian; Hope Serepa-Dlamini, Mahloro

doi:10.1016/j.heliyon.2024.e30736

Heliyon

2024

DOI: 10.1016/j.heliyon.2024.e30736

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Screening of Bacillus spp. bacterial endophytes for protease production, and application in feather degradation and bio-detergent additive

Malese Elaine Mankge,

Mehabo Penistacia Maela,

Adrian Mark Abrahams

et al.

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2024

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Cited by 3 publications

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Production of keratinase enzyme from B. subtilis GH2 and its application in feather biodegradation

Alamnie

2024

Biomass Conv. Bioref.

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Production of keratinase enzyme from B. subtilis GH2 and its application in feather biodegradation

Alamnie

2024

Biomass Conv. Bioref.

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Economic production of eco-friendly dehairing keratinase and antioxidant feather hydrolyzate using Bacillus halotolerans

Ismail,

Nour,

Nashy

et al. 2024

Biomass Conv. Bioref.

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Feather is a high recalcitrance keratin-based biomass, and keratin-degrading microorganisms have been seen as beneficial tools for its biodegradation and production of valuable products with biomedical and industrial applications. In the current study, the isolated keratinolytic bacteria was identified on the base of its 16 S rDNA sequencing data as Bacillus halotolerans, and its efficiency in the hydrolysis of feather with the preservation of most of its amino acid constituent was estimated by high-performance liquid chromatography Pico-Tag (HPLC PICO-TAG) method. The produced hydrolysate possessed antioxidant activity of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity of 52.3 ± 5.6 mmole TE/g without possessing any cytotoxicity to human normal skin fibroplast cell line BJ-1 estimated by MTT assay. Moreover, the isolated strain was capable for producing keratinase under submerged fermentation of feather that was optimized by combining single-variable-at-a-time optimization with the application of statistical designs (Plackett-Burman and Box-Behnken). The optimum keratinase activity was 140.83 U/mL that was enhanced by 3.2-fold of the estimated initial activity, and the purified enzyme possessed optimum activity at pH 9.5 and 70 °C with a half-life of 69.3 min. Finally, the applicability of the purified enzyme in the dehairing of bovine hide was examined in comparison to sodium sulfide (Na2S) and lime (Ca(OH)2) conventional method. The results estimated the efficiency of the examined bio-treatment process, it achieved complete dehairing after 2 h with a good skin quality as manifested by scanning electron microscope (SEM). In addition, a significant reduction of the pollution load parameters was estimated.

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Enzymatic modification of cotton fibre polysaccharides as an enabler of sustainable laundry detergents

Yau,

Byard,

Thompson

et al. 2024

Sci Rep

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Cotton is the most common natural fibre used in textile manufacture, used alone or with other fibres to create a wide range of fashion clothing and household textiles. Most of these textiles are cleaned using detergents and domestic or commercial washing machines using processes that require many chemicals and large quantities of water and energy. Enzymes can reduce this environmental footprint by enabling effective detergency at reduced temperatures, mostly by directly attacking substrates present in the soils. In the present study, we report the contribution of a cleaning cellulase enzyme based on the family 44 glycoside hydrolase (GH) endo-beta-1,4-glucanase from Paenibacillus polymyxa. The action of this enzyme on textile fibres improves laundry detergent performance in several vectors including soil anti-redeposition, dye transfer inhibition and stain removal. Molecular probes are used to study how this enzyme is targeting both amorphous cellulose and xyloglucan on textile fibres and the relationship between textile surface effects and observed performance benefits.

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Screening of Bacillus spp. bacterial endophytes for protease production, and application in feather degradation and bio-detergent additive

Cited by 3 publications

References 64 publications

Production of keratinase enzyme from B. subtilis GH2 and its application in feather biodegradation

Production of keratinase enzyme from B. subtilis GH2 and its application in feather biodegradation

Economic production of eco-friendly dehairing keratinase and antioxidant feather hydrolyzate using Bacillus halotolerans

Enzymatic modification of cotton fibre polysaccharides as an enabler of sustainable laundry detergents

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