Screening of Australian Plants for Antimicrobial Activity against Campylobacter jejuni

Abstract

Campylobacter jejuni is the most common cause of acute enteritis in humans, with symptoms such as diarrhoea, fever and abdominal cramps. In this study, 115 extracts from 109 Australian plant species were investigated for their antimicrobial activities against two C. jejuni strains using an in vitro broth microdilution assay. Among the plants tested, 107 (93%) extracts showed activity at a concentration between 32 and 1024 µg/mL against at least one C. jejuni strain. Seventeen plant extracts were selected for further testing against another six C. jejuni strains, as well as Campylobacter coli, Escherichia coli, Salmonella typhimurium, Bacillus cereus, Proteus mirabilis and Enterococcus faecalis. The extract from Eucalyptus occidentalis demonstrated the highest antimicrobial activity, with an inhibitory concentration of 32 µg/mL against C. jejuni and B. cereus. This study has shown that extracts of selected Australian plants possess antimicrobial activity against C. jejuni and thus may have application in the control of this organism in live poultry and retail poultry products.

Antimicrobial Potential of Wild Edible Herbaceous Species

“…The extracts were found active against Campylobater jejuni, one of the most common causes of acute enteritis in humans (Kurekci et al 2012).…”

Antimicrobial activity of essential oils and five terpenoid compounds against Campylobacter jejuni in pure and mixed culture experiments

“…Experiments were carried out three times for reproducibility and MICs were determined spectrophotometrically by measuring the bacterial concentration at an absorbance of A 600 nm. Iodonitrotetrazolium chloride (40 μL of 0.2 mg/ml) was also employed as a growth indicator at the end of the incubation period as described for antimicrobial plant extracts (Kurekci et al, 2012). Pink colour indicates growth while no colour change means inhibition of growth after incubation at 37°C for 30 min.…”

“…The range of test concentration chosen was based on the reports reviewed by Carson et al (2006). Bacterial inoculum (190 μl) was prepared as previously described (Kurekci et al, 2012) and added into the wells containing 10 μl serial dilutions of EOs/compounds resulting in a final bacterial concentration of 3-5 × 10 5 CFU/ml. Tween 80 was also employed to increase the solubility of oils and used at a final concentration of 2 × 10 −3 % (v/v).…”

“…E. coli 026 was obtained as a human clinical isolate (Al-Ajmi et al, 2006). All cultures were grown as previously described (Kurekci et al, 2012). Campylobacter isolates were cultured on blood agar plates [40 g/L blood agar base no.…”

Synthesis and Biological Evaluation of New (−)-Gossypol-Derived Schiff Bases and Hydrazones

“…In order to expand the biological spectrum of gossypol derivatives, synthesized hydrazones were also evaluated for antibacterial activity against human pathogenic bacteria, including Staphylococcus aureus (ATCC 6538), Bacillus cereus (ATCC 21768), Bacillus subtilis (ATCC 6633), Escherichia coli (ATCC 25931), and Pseudomonas aeruginosa (ATCC 9027), according to described protocol [26]. Briefly, five bacterial species including two Gram-negative strains (Escherichia coli ATCC 25931 and Pseudomonas aeruginosa ATCC 9027) and three Gram-positive strains (Bacillus subtilis ATCC 6633, Bacillus subtilis ATCC 6633, and Staphylococcus aureus ATCC 6538) were obtained from the ATCC (Manassas, VA, USA).…”