Screening of Argentine marine algae for antimicrobial activity

Espeche, Maria E.; Fraile, Eida R.; Mayer, Alejandro M. S.

doi:10.1007/978-94-009-6560-7_107

Cited by 8 publications

(7 citation statements)

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“…An extended spectrum of action was observed for sixteen taxa compared to studies performed with non-Mediterranean (Baker, 1984;Espeche et al, 1984;Reichelt and Borowitzka, 1984;Usmanghani et al, 1984;Hornsey and Hide, 1985;Ballantine et al, 1987;Navarro et al, 1990;Padmakumar and Ayyakkannu, 1997) as well as Mediterranean samples (Caccamese et al, 1980(Caccamese et al, , 1981(Caccamese et al, , 1985Serarols et al, 1982;Moreau et al, 1984;Ballesteros et al, 1992) (Table 5). Dictyopteris polypodioides, Halimeda tuna and Hypnea musciformis, three taxa reported as active in other surveys of nonMediterranean samples (Hornsey and Hide, 1974;Sreenivasa Rao and Parekh, 1981;Usmanghani et (Seguin et al, 1995;Algues et Mer 2002).…”

Section: Discussionmentioning

confidence: 90%

Antimicrobial activity of Iberian macroalgae

Salvador¹,

Garreta²,

Lavelli³

et al. 2007

Sci. Mar.

177

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SUMMARY: The antibacterial and antifungal activity of 82 marine macroalgae (18 Chlorophyceae, 25 Phaeophyceae and 39 Rhodophyceae) was studied to evaluate their potential for being used as natural preservatives in the cosmetic industry. The bioactivity was analysed from crude extracts of fresh and lyophilised samples against three Gram-positive bacteria, two Gram-negative bacteria and one yeast using the agar diffusion technique. The samples were collected seasonally from Mediterranean and Atlantic coasts of the Iberian Peninsula. Of the macroalgae analysed, 67% were active against at least one of the six test microorganisms. The highest percentage of active taxa was found in Phaeophyceae (84%), followed by Rhodophyceae (67%) and Chlorophyceae (44%). Nevertheless, red algae had both the highest values and the broadest spectrum of bioactivity. In particular, Bonnemaisonia asparagoides, Bonnemaisonia hamifera, Asparagopsis armata and Falkenbergia rufolanosa (Bonnemaisoniales) were the most active taxa. Bacillus cereus was the most sensitive test microorganism and Pseudomonas aeruginosa was the most resistant. The highest percentages of active taxa from Phaeophyceae and Rhodophyceae were found in autumn, whereas they were found in summer for Chlorophyceae.Keywords: antimicrobial activity, marine macroalgae, Bonnemaisoniales, agar diffusion technique, crude extracts, Iberian Peninsula.RESUMEN: ACTIVIDAD ANTIMICROBIANA DE MACROALGAS MARINAS DE LA PENÍNSULA IBÉRICA. -Se analizó la actividad antibacteriana y antifúngica de 82 macroalgas marinas (18 Chlorophyceae, 25 Phaeophyceae y 39 Rhodophyceae) para valorar su potencial aplicación como conservantes naturales en la industria cosmética. Los extractos crudos de cada taxon, preparados tanto a partir de material fresco como liofilizado, fueron testados frente a tres bacterias Gram positivas, dos bacterias Gram negativas y una levadura, mediante la técnica de difusión en agar. Las muestras fueron recolectadas en diversas localidades de las costas mediterráneas o atlánticas de la Península Ibérica en distintas estaciones del año. El 67% de todas las macroalgas estudiadas mostraron actividad antimicrobiana frente al menos un microorganismo test de los seis utilizados. El mayor porcentaje de táxones activos lo presentó el grupo de las Phaeophyceae (84%) seguido por las Rhodophyceae (67%) y por las Chlorophyceae (44%). No obstante, las algas rojas fueron las que presentaron el mayor grado de actividad así como el espectro de acción más amplio y, dentro de este grupo, Bonnemaisonia asparagoides, Bonnemaisonia hamifera, Asparagopsis armata y Falkenbergia rufolanosa (Bonnemaisoniales) fueron los táxones más activos. En cuanto a los microorganismos, Bacillus cereus fue el más sensible y Pseudomonas aeruginosa el más resistente. Los tres grupos taxonómicos mostraron una variación estacional en la producción de sustancias antimicrobianas, siendo el otoño la estación con mayor porcentaje de táxones activos para las Phaeophyceae y Rhodophyceae, mientras que para las Chlorophyceae fue...

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Section: Discussionmentioning

confidence: 90%

Antimicrobial activity of Iberian macroalgae

Salvador¹,

Garreta²,

Lavelli³

et al. 2007

Sci. Mar.

177

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“…(Margesin et al 1991 ;Schinner et al 1992 ;Vazquez et al 1995) bacteria have been reported. Notably, psychrotrophic bacteria isolated from Antarctic krill (Espeche et al 1987) or Identification of protease-producing bacteria fish intestine include many strains producing extracellular Preliminary identification of the strains was achieved by proteases. These results suggest that protease-producing bacGram staining, motility and the oxidase reaction.…”

Section: Macrocephalus) and Sand Flounder (Pleuronectes Punctatissimumentioning

confidence: 99%

Isolation of a Pseudomonas species from fish intestine that produces a protease active at low temperature

Hashizume

Ishizaki

Sakamoto

et al. 1997

Letters in Applied Microbiology

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“…Reichelt and Borowitzka 1984). Strong antibiotic activity has been detected in extracts from several algal species, whereas only weak or negligible effects were found in others (Caccamese and Azzolina 1979, Espeche et al 1984, Pesando and Caram 1984, Reichelt and Borowitzka 1984. Moreover, most antibiotic effects detected in algal extracts varied with seasons, algal life history stage, and the age of tissues examined (Jones 1988).…”

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confidence: 99%

OLIGOAGARS ELICIT A PHYSIOLOGICAL RESPONSE IN GRACILARIA CONFERTA (RHODOPHYTA)

Weinberger¹,

Friedländer²,

Höppe³

1999

Journal of Phycology

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Gracilaria conferta (Schousboe ex Montagne) J. et G. Feldmann responded with an oxidative burst and rapid increases in respiration and halogenating activity when agar, agarose, or the agarose degradation products neoagarotetraose and neoagarohexaose were added to the growth medium. In contrast, carrageenan, oligocarrageenans, neoagarobiose, L-galactose, D-galactose, and several other mono-and oligosaccharides did not have any effect. Sixfold increases in respiration were observed 3 min after addition of neoagarohexaose. The response could only be induced in species of the genera Gracilaria and Gracilariopsis. Neoagarohexaose also elicited a release of hydrogen peroxide in less than 15 min, resulting in an immediate increase in algal brominating activity. Bleached thallus tips appeared a few hours after the addition of neoagarohexaose. This effect was dependent on the release of hydrogen peroxide and exposure to light. Exposure to light and oligosaccharide elicitors increased the production of reactive oxygen species, which reached destructive concentrations when both mechanisms were simultaneously active. Concentrations of 0.1 to 3.3 M agarose or agars were sufficient to trigger an increase in respiration, an oxidative burst response, and tip bleaching. However, higher concentrations of neoagarohexaose and neoagarotetraose were necessary to elicit the responses, indicating that the alga is more sensitive to oligoagars with degrees of biose-polymerization Ͼ 3. The extremely short reaction time and high specificity indicate that intermediates of agar degradation are recognized by Gracilaria as messengers when microbial degradation of its cell wall occurs. The physiological responses may represent the early stages of algal defense mechanisms involved in repression of pathogen ingress.

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Screening of Argentine marine algae for antimicrobial activity

Cited by 8 publications

References 1 publication

Antimicrobial activity of Iberian macroalgae

Antimicrobial activity of Iberian macroalgae

Isolation of a Pseudomonas species from fish intestine that produces a protease active at low temperature

OLIGOAGARS ELICIT A PHYSIOLOGICAL RESPONSE IN GRACILARIA CONFERTA (RHODOPHYTA)

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