Screening of Antibacterial Properties for Some Iraqi Plants Against Salmonella typhimurium

Abstract

This study was include the investigation of the antibacterial activity of ethanolic extract for (12) type of Iraqi plant leaves (Allium porrum L, Apium graveolens Mill., Cassia angustifollia Vahl., Lawsonia inermis L., Lepidium sativum L., Malva sylvestris L., Monthapiperita L., Ocimmum bassillicum L., Pettroselinum crispum Mill., Raphanus sativus L., Sponacia oleraceal. and Trigonella foenum L.). In different concentrations (20, 40, 60, 80, 100) mg/ml against Salmonella typhimurium in vitro using agar well diffusion method. All ethanolic extract of leaves showed a high activity against this bacterium except of Cassia angustifollia Vahl. as compared with the other extracts. The ethanolic extract of leaves of Petroselinum crispum Mill was showed the best activity with inhibition zone of twenty three mm in the concentration (100) mg/ml. This results confirm the antibacterial activity of these plants, and leaf components which are responsible for this action have to be isolated for using as therapeutic agents.Conclusion: The present investigation together with previous studies provide support to the anti-Salmonella typhimurium properties of studied plant leaves. It can used as antibacterial supplement towards the development of the new chemotherapy . Additional in vivo studies and clinical trails would be needed to justify and further evaluate the potential of these plants as anti-Salmonella typhimurium agent in treatment of infectious diseases caused by this bacteria.

Antimicrobial Effects of Medicinal Plant Species on Salmonella typhimurium Strains Isolated from Poultry Feces Samples

“…The potent antibacterial potential of M. sylvestris, as witnessed in the present study, agrees with the reports on it against various bacteria, including Salmonella (29,30). Moreover, the MIC/MBC of M. sylvestris extract against the standard and clinically isolated Salmonella enterica from diarrheic lambs in Urmia, Iran, were reported to be 50/100 and 42/80 mg/mL, respectively (31).…”

The Role of Nisin, Potassium Sorbate and Sodium Lactate as Additive in Improving the Chemical and Qualitative Characteristics of Chilled Ground Beef.

“…This may be due to the fact that T6 (nisin 150 mg/kg + 3% sodium lactate) treatment is the best in inhibiting the total number of bacteria, psychrophilic bacteria and coliform bacteria. As for the periods of cold storage, table (7) shows a significant increase (P˂0.01) in the logarithm of the total number of bacteria in the period 12, 8 and 4 days as compared to the period 0 days of cold storage and this results came in agreement with those of (10, 13,25,34,36). It is noted from the results that the total number of bacteria for all addition treatments was within permissible limits for the total count of bacteria in meat by the Iraqi Central Organization for Standardization and Quality Control (7 logarithmic cycles) (16).…”

Phytochemical Composition, Antibacterial, and Antibiofilm Activity of Malva sylvestris Against Human Pathogenic Bacteria

“…In a study conducted by Shadid et al (13), the acetonic extract of M. sylvestris exhibited strong antibacterial activity against Proteus vulgaris and S. aureus with the MIC values of 0.078 and 0.125 mg/mL, respectively. Other studies have also shown the cytotoxic properties of M. sylvestris extract against Salmonella enterica serovar Typhimurium (14), Listeria monocytogenes (15), Bacillus cereus (15), methicillin-resistant S. aureus (MRSA) (11), Aggregatibacter actinomycetemcomitans (10), and Candida species (16). The limitation of previous studies is that in all these studies, only the antimicrobial properties of the extract were investigated, and its anti-biofilm properties on pathogenic bacteria were not evaluated.…”