Screening of anti-cancer agent using zebrafish: Comparison with the MTT assay

Li, Yigen; Huang, Weidong; Huang, Shenyuan; Du, Jiulin; Huang, Cheng

doi:10.1016/j.bbrc.2012.04.110

Cited by 72 publications

(42 citation statements)

References 26 publications

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“…The embryos are transparent and it is possible to study the effect of a molecule on different organs at the same time on the living animal and hundreds of transgenic lines are available (Novodvorsky et al 2013). Zebrafish is an efficient model not only for the drug discovery process but also for target validation, toxicity studies and drug optimization (Li et al 2012). …”

Section: High-throughput Screening (Hts) Versus Evidenced-based Tradimentioning

confidence: 99%

The pharmaceutical industry and natural products: historical status and new trends

2014

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Section: High-throughput Screening (Hts) Versus Evidenced-based Tradimentioning

confidence: 99%

The pharmaceutical industry and natural products: historical status and new trends

2014

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“…Previous studies using zebrafish have identified significant effects of rapamycin on autophagy (15), prevention of hepatic steatosis (16), heart development (17) and demonstrated the importance of zebrafish as a mitochondrial and ribosomal disease model (18,19). Although the zebrafish is an emerging model in drug-screening and in vivo disease models (20), evolutionarily conserved effects of rapamycin on the zebrafish transcriptome in addition to the dose-dependency in embryonic/larval size and pigmentation have not previously been studied.…”

Section: Introductionmentioning

confidence: 99%

Functionally conserved effects of rapamycin exposure on zebrafish

Sucularli¹,

Shehwana²,

Kuscu³

et al. 2016

Molecular Medicine Reports

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Abstract. Mechanistic target of rapamycin (mTOR) is a conserved serine/threonine kinase important in cell proliferation, growth and protein translation. Rapamycin, a well-known anti-cancer agent and immunosuppressant drug, inhibits mTOR activity in different taxa including zebrafish. In the present study, the effect of rapamycin exposure on the transcriptome of a zebrafish fibroblast cell line, ZF4, was investigated. Microarray analysis demonstrated that rapamycin treatment modulated a large set of genes with varying functions including protein synthesis, assembly of mitochondrial and proteasomal machinery, cell cycle, metabolism and oxidative phosphorylation in ZF4 cells. A mild however, coordinated reduction in the expression of proteasomal and mitochondrial ribosomal subunits was detected, while the expression of numerous ribosomal subunits increased. Meta-analysis of heterogeneous mouse rapamycin microarray datasets enabled the comparison of zebrafish and mouse pathways modulated by rapamycin, using Kyoto Encyclopedia of Genes and Genomes and Gene Ontology pathway analysis. The analyses demonstrated a high degree of functional conservation between zebrafish and mice in response to rapamycin. In addition, rapamycin treatment resulted in a marked dose-dependent reduction in body size and pigmentation in zebrafish embryos. The present study is the first, to the best of our knowledge, to evaluate the conservation of rapamycin-modulated functional pathways between zebrafish and mice, in addition to the dose-dependent growth curves of zebrafish embryos upon rapamycin exposure.

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“…The MTT assay was conducted as described by Li et al (2012). In brief, exponentially growing cells at 1 × 10 5 cells/ml were seeded in 96-well microplates and treated with siRNA (pSilencer-Smad2 siRNA or pSilencer-Smad3 siRNA) or inhibitor (SB203580).…”

Section: Mtt Assaymentioning

confidence: 99%