Screening of antagonistic activity of microorganisms against Colletotrichum acutatum and Colletotrichum gloeosporioides

Živković, Svetlana; Stojanović, Saša; Ivanović, Žarko; Gavrilović, Veljko; Popović, Tatjana; Balaž, Jelica

doi:10.2298/abs1003611z

Cited by 139 publications

(77 citation statements)

References 21 publications

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“…Therefore, it was not surprising to find out that the isolated endophytic bacteria mostly came from root. [21]. The other isolates were categorized as having moderate antagonism activity as they had PIRG value of below 76%.…”

Section: Resultsmentioning

confidence: 99%

Biocontrol Potential of Endophytic Bacteria Isolated from Healthy Rice Plant against Rice Blast Disease (Pyricularia oryzae Cav.)

Widiantini¹,

Herdiansyah²,

Yulia³

2017

KLS

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Isolation was attempted to collect endophytic bacteria as potential biocontrol agents against rice blast disease (Pyricularia oryzae Cav.). The disease is one of major threats in rice production as it can cause 100% yield loss. Concern on the environment and human health has led to the searching of alternative controlling method to replace the commonly used pesticide-based method. Endophytic bacteria are bacteria that have intimate relationship with their host without inducing any pathogenic symptom.The use of endophytic microbial as biocontrol agent has its own advantages as the microbes are more easily to adapt to the environment needed by the host plant. We evaluated endophytic bacteria isolated from healthy rice plants and tested for their potential biocontrol activity using dual culture assay. Ten isolates were found to inhibit the growth of P. oryzae of more than 50%. Microscopic observation showed that they were able to cause the mycelia malformation of P. oryzae. Further work is currently in progress to determine their effectiveness in the pot trial.

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Section: Resultsmentioning

confidence: 99%

Biocontrol Potential of Endophytic Bacteria Isolated from Healthy Rice Plant against Rice Blast Disease (Pyricularia oryzae Cav.)

Widiantini¹,

Herdiansyah²,

Yulia³

2017

KLS

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“…Meanwhile, Bacillus amyloliquefaciens isolated from tomatoes acquired longer cultivation periods of 48 hours to inhibit Alternaria solani, a pathogenic fungus which caused early blight in tomato [16]. The rapid growth of endophytic bacteria offered advantage in the race for space and nutrients against pathogenic fungi, before any antifungal activity was deployed [17]. Plant growth hormones extracted from B. amyloliquefaciens LKM-UL using ethyl acetate fraction on TLC plate showed three clear individual spots and several faded spots of indole (auxins) and gibberellins hormones (Fig 2a).…”

Section: International Journal Of Bioscience Biochemistry and Bioinfmentioning

confidence: 99%

Antagonistic Effect and Plant Growth Hormone Produced by Endophyte Bacillus amyloliquefaciens LKM-UL Isolated from Cocoa Plant

Hamzah¹,

Zubir²,

Ross³

et al. 2017

IJBBB

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Abstract:In Malaysia black pod disease caused by Phytophthora palmivora is one of the serious problems causing yield loss in cocoa plantation. In this study, an endophytic bacterium living within tissues of healthy Theobroma cacao L. leaves was isolated and assessed its ability to inhibit the growth of P. palmivora. Based on molecular identification using 16S rDNA gene sequences analysis, this endophytic bacterium was identified as Bacillus amyloliquefaciens which was subsequently named as Bacillus amyloliquefaciens LKM-UL. Preliminary test using dual culture method showed B. amyloliquefaciens LKM-UL has strong ability to inhibit the growth of P. palmivora. B. amyloliquefaciens showed the highest antifungal activity after 24 h incubation in nutrient broth. Cells-free supernatant from B. amyloliquefaciens showed the highest antifungal activity of 30.6 mm with cell concentration of Log 10 10.63 CFU/mL via agar disc diffusion method. B. amyloliquefaciens LKM-UL also produced plant growth hormone identified as 1-naphthalene acetic acid (NAA), tryptamine, 3-indole propionic acid (IPA), indole-3-butyric acid (IBA), gibberellic acid (GA), trans-zeatin and kinetin.

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“…Penggunaan Bacillus sp. baik sebagai agens hayati maupun PGPR telah banyak digunakan untuk mengendalikan patogen seperti Fusarium sp., Botrytis cinerea, Pythium sp., Colletotrichum acutatum, C. gloeosporioides, F. graminearum, Meloidogyne incognita, dan R. solanacearum (Kim et al 2003;Domenech et al 2006;Bustamam 2006;Nourozian et al 2006;Arwiyanto et al 2007;Zivkovic et al 2010). Untuk aplikasi di lapangan, penambahan agens hayati seperti Bacillus sp., Pseudomonas fluorescens, Trichoderma sp., Pennicilium digitatum, dan Gliocladium virens pada pupuk kandang dapat menekan kejadian penyakit layu bakteri pada tanaman jahe dan meningkatkan pertumbuhan tanaman (Bustamam 2006).…”

Section: Hasil Dan Pembahasanunclassified

Uji Antagonisme Bacillus cereus terhadap Rhizoctonia solani dan Sclerotium rolfsii

Hidayah

Yulianti

2016

Bul. Tan. Tembakau, Serat & Minyak Industri

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ABSTRAKJamur Rhizoctonia solani dan Sclerotium rolfsii merupakan kelompok jamur steril (tidak menghasilkan spora) tetapi dapat menghasilkan sklerosia sebagai sumber inokulum primer, dan struktur istirahat jamur yang dapat bertahan selama beberapa tahun di dalam tanah saat kondisi lingkungan kurang menguntungkan. Penggunaan fungisida, fumigasi, dan solarisasi tanah telah digunakan untuk mengendalikan kedua jamur tersebut, namun hasil yang diperoleh masih beragam. Pengendalian hayati dengan menggunakan bakteri Bacillus sp. yang merupakan salah satu kelompok agens hayati patogen diketahui memberikan hasil yang baik pada beberapa tanaman. Penelitian yang bertujuan menguji potensi B. cereus dalam menghambat pertumbuhan jamur R. solani dan S. rolfsii secara in vitro dilaksanakan di Laboratorium Fitopatologi Balittas dengan menggunakan metode dual culture pada media potato dextrose agar (PDA). Miselia jamur R. solani dan S. rolfsii masing-masing berumur 5 hari diambil dengan menggunakan cork borer ukuran 0,5 cm ditanam pada media PDA berhadapan dengan B. cereus dengan jarak 3 cm. Penelitian disusun dalam rancangan acak lengkap dan diulang empat kali. Pengamatan dilakukan terhadap persentase penghambatan pertumbuhan jamur oleh Bacillus sp. dan laju pertumbuhan jamur. Hasil penelitian menunjukkan bahwa Bacillus sp. mampu menghambat pertumbuhan miselia R. solani dan S. rolfsii masing-masing sebesar 68,9% dan 33% pada hari ketiga setelah perlakuan. Keberadaan B. cereus dapat memperlambat laju pertumbuhan R. solani (15,5 mm/ 24 jam), dibandingkan perlakuan kontrol (tanpa B. cereus) sebesar 19,7 mm/24 jam. Hasil ini menunjukkan bahwa B. cereus dapat menghambat pertumbuhan R. solani dan berpotensi untuk dikembangkan sebagai agens hayati.Kata kunci: Bacillus sp., pengendalian hayati, Rhizoctonia solani, Sclerotium rolfsii, rebah kecambah ABSTRACTRhizoctonia solani and Sclerotium rolfsii (the causal agents of damping off disease on various hosts) are the group of sterile fungi that cannot produce spores. Nevertheless, they produce sclerotia as primary inocula and resting spores when facing unfavorable condition. Several control methods using chemical fungicides and solarization had been conducted, but the results were still inconsistent. In addition, the use of Bacillus sp. as a biological control agent for several plant diseases had provided successful results. Furthermore, the research aimed to evaluate the potency of B. cereus towards R. solani and S. rolfsii in vitro was carried out in the laboratory of phytopathology using dual culture method on PDA medium. Five days of R. solani and S. rolfsii miselia were plugged and inoculated on PDA medium toward B. cereus. The research was arranged by completely randomized design with four replicates. The percentage of fungal inhibition and fungal growth rate were observed. The result showed that B. cereus exhibited mycelial growth inhibition activity of R. solani and S. rolfsii by 68,9% and 33% three days after treatments, respectively. The result also indicated that B. cereus has a ...

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Screening of antagonistic activity of microorganisms against Colletotrichum acutatum and Colletotrichum gloeosporioides

Cited by 139 publications

References 21 publications

Biocontrol Potential of Endophytic Bacteria Isolated from Healthy Rice Plant against Rice Blast Disease (Pyricularia oryzae Cav.)

Biocontrol Potential of Endophytic Bacteria Isolated from Healthy Rice Plant against Rice Blast Disease (Pyricularia oryzae Cav.)

Antagonistic Effect and Plant Growth Hormone Produced by Endophyte Bacillus amyloliquefaciens LKM-UL Isolated from Cocoa Plant

Uji Antagonisme Bacillus cereus terhadap Rhizoctonia solani dan Sclerotium rolfsii

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