Screening of Agricultural Raw Materials and Long-Term Storage Products to Identify Bacillary Contaminants

Danylova, Olena; Serdyuk, Мarina; Pylypenko, L.; Pelykh, V.H.; Lopotan, Inna; Iegorova, Antonina

doi:10.1007/978-3-030-14918-5_63

Cited by 3 publications

(3 citation statements)

References 6 publications

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“…The mussel juice was obtained by manual cleaning the shells of mussels. The initial volume of mussel juice, comprising 100 cm 3 , was centrifuged at 4000 rpm for 30 minutes in order to remove fragments of shells, tissue parts and mussel meat. Lysozyme activity was determined spectrophotometrically [21; 22], the Lowry protein was modified by Hartree [23].…”

Section: Methodsmentioning

confidence: 99%

“…It is known that during the storage of readyto-eat foods various physical, chemical and microbiological changes occur, affecting their quality indicators and shelf life. Food products are often exposed to surface contamination, leading to a reduction in shelf life [1][2][3]. The main cause of food spoilage is the microbial contamination of their surface.…”

Section: Introductionmentioning

confidence: 99%

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Виділення Лізоциму Чорноморської Мідії Mytilus Galloprovincialis

Курсир¹,

Захарчук²,

Севастьянова³

et al. 2021

J. of Chem. and Tech.

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Аналітичний огляд показав перспективність використання лізоциму як цінного природного антибактеріального препарату. Експериментальний моніторинг природних джерел препаратів з антибактеріальною активністю виявив доцільність використання вторинної сировини з водних ресурсів чорноморського басейну, отриманого в процесі переробці мідій. Проведено виділення і хроматографічне очищення ферментного препарату лізоциму. Визначено, що тільки зразки, елюйовані 0.6 М розчином NaСl в 0.02 М ацетатному буфері, pH 5.0, виявляють літичну активність. Фракція з літичною активністю склала 54 см3 при елююванні 0.6 М розчином NaCl в 0.02 М ацетатному буфері, pH 5.0. Лізоцим, досліджений за результатами гель-електрофорезу в 15 % ПААГ з використанням калібрувальної кривої, характеризується молекулярною масою 18 кДа, що підтверджує його приналежність до низькомолекулярних білків. Отримано препарат лізоциму з соку чорноморських мідій з питомою активністю 206 од / мг, молекулярна маса якого становить 18 кДа, ступінь очищення – 946.6. Досліджено антибактеріальну активність отриманого препарату лізоциму до збудників харчових отруєнь і псування харчових продуктів. Отримані результати дозволяють прогнозувати перспективи використання мідій як джерела високоактивного лізоциму.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Виділення Лізоциму Чорноморської Мідії Mytilus Galloprovincialis

Курсир¹,

Захарчук²,

Севастьянова³

et al. 2021

J. of Chem. and Tech.

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“…where, [Іn Н] = the concentration of the inhibitor obtained experimentally, and t = the duration of the induction period, s. Microbiological parameters were determined by classical as well as accelerated PCR methods using microbiological media Compact Dry (Nissui Pharmaceutical Co. Ltd., Japan) (Kapreliants et al, 2016;Pylypenko et al, 2017;Berhilevych et al, 2019;Danylova et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

New high-fat dairy products with colour attractants

Pylypenko¹,

Sevastyanova²,

Makovska³

et al. 2021

IFRJ

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In the present work, the nutritional value of high-fat dairy product - melted butter, which was produced in Ukraine, was characterised. It was shown that, along with the traditional fatty acid compositions, this product had an insufficient concentration of biologically active substances that contributes to storage stability, expands organoleptic characteristics, and prevents main spoilage processes such as rancidity and tallowiness. The appropriate biologically active colouring attractants for improvement of organoleptic and biological properties of melted butter were observed. The formulations of new melted butter-based products were tested by using two individual carotenoids (carotene and xanthophyll); namely chlorophyll and chlorophyll-carotenoid complex additives in a concentration of 3 - 7%. The antioxidant activity of the samples, which was determined volumetrically by the intensity of initiated oxidation of isopropyl benzene, made it possible to establish an increase (4.40 - 6.52 times the period of induction) of oxidation for melted butter variants, with colorants. The study of organoleptic, physico-chemical, and microbiological indicators confirmed the biological stability and safety of the developed high-fat dairy products, thus fulfilling the body requirement for carotenoids, magnesium, and several other biologically active compounds. According to the results, the guaranteed shelf life of the developed melted butter types with colouring attractants of carotenoid and chlorophyll, which met the safety requirements, could be prolonged by 31 - 48%. In conclusion, the present work improved the technical and economic indicators, and confirmed the feasibility of their production.

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Age Changes in the Body Weight of Ram Lambs Obtained From Ewes of Different Body Types

Kitaeva,

Bezaltychna,

Novichkova

2024

STBIAS

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The research was carried out in the production conditions of the private farm “AGRO-DIS” of the Podilsky district of the Odesa oblast with the population of crossbred F1 rams, obtained from the industrial crossing of ewes of the Askaniysky meat-wool breed with crossbred wool of the Odesa inbred type of different body types with Merinolandschaf rams. Changes in body weight of ram lambs from birth to 18 months old were studied according to generally accepted methods. It was established that the type of birth – singles or twins – affects the body weight of crossbred lambs. So, at birth, single lambs, offspring of ewes with a strong body type, had the largest body weight compared to peers of other body types. Their advantage compared to peers obtained from ewes of a slender body type was 1.3 kg or 26.42% (Р>0.999), and compared to rams of a corpulent body type, it was 0.59 kg or 10.48% (Р>0, 95). However, in later age periods of growth, peers of the corpulent body type dominated in terms of body weight. Crossbred rams (AMO x ME) in all age periods of growth had a high body weight regardless of the type of birth and the body type of the ewes. The difference in body weight of single rams, offspring from ewes of strong and corpulent body type was greater in favor of rams obtained from ewes of corpulent body type with a high degree of probability and amounted to: in 4 months. – 11.54 kg or 26.58% (Р>0.999), 8 months. – 9.29 kg or 18.8% (Р>0.999), 12 months. –14.26 kg or 23.2% (Р>0.999), 16 months. – 10.39 kg or 14.35% (Р>0.999), 18 months. – 11.15 kg or 13.93% (Р>0.999). Offspring born by ewes with a corpulent body type also prevailed over peers obtained from ewes of a slender body type at all age periods of growth. The obtained results of research indicate the expediency of industrial crossbreeding at farms with the aim of increasing the meat productivity of sheep.

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Screening of Agricultural Raw Materials and Long-Term Storage Products to Identify Bacillary Contaminants

Cited by 3 publications

References 6 publications

Виділення Лізоциму Чорноморської Мідії Mytilus Galloprovincialis

Виділення Лізоциму Чорноморської Мідії Mytilus Galloprovincialis

New high-fat dairy products with colour attractants

Age Changes in the Body Weight of Ram Lambs Obtained From Ewes of Different Body Types

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