Screening of a Novel Fibrinolytic Enzyme-Producing Streptomyces from a Hyper-Arid Area and Optimization of Its Fibrinolytic Enzyme Production

Abstract

Fibrinolytic enzymes are a kind of proteolytic enzymes that can hydrolyze fibrin and dissolve blood clots. They could be used as a therapeutic agent for treating thrombosis. It is important for the treatment of cardiovascular disease to find and develop new thrombolytic drugs. In order to explore new fibrinolytic enzymes, a strain named 214L-11 with protease and fibrinolytic enzyme activity, which was isolated from the Flaming Mountain of Xinjiang Province, was screened using the skimmed milk plate, the blood powder agarose plate and the fibrin plate methods. Phylogenetic analyses showed that strain 214L-11 shared the highest similarity with Streptomyces fumanus NBRC 13042T (98.88%), which indicated that it represented a potential novel species in the Streptomyces genus. The fibrinolytic enzyme produced by 214L-11 displayed thrombolytic and anticoagulant activities, and it could degrade a single specific protein in the thrombus, thereby destroying the thrombus structure. The fermentation medium optimized through response surface methodology was 15 g/L soluble starch, g/L KNO3 0.58, 0.43 g/L peptone, 0.01 g/L FeSO4·7H2O, 0.5 g/L MgSO4·7H2O, 0.2 g/L Mn2+, 0.5 g/L NaCl and 1 L distilled water, pH 8, and the maximum amount of fibrinolytic enzyme produced by strain 214L-11 in the optimal fermentation medium was 1255.3 FU/mL. Overall, the fibrinolytic enzyme-producing strain was screened from the Flaming Mountain of Xinjiang for the first time, which provided a basis for further research and the development of new efficient and safe hemolytic drugs.

Modeling and Optimization of the Culture Medium for Efficient 4′-N-Demethyl-Vicenistatin Production by Streptomyces parvus Using Response Surface Methodology and Artificial-Neural-Network-Genetic-Algorithm

“…The composition and concentration of the medium can exert a significant influence on microbial fermentation production [45][46][47]. This is particularly applicable to microbial secondary metabolic processes, where the composition and concentration of the carbon sources in the medium can significantly impact the fermentation of secondary metabolites, such as pigment fermentation and antibiotic production [48][49][50]. The choice of carbon source can exert a significant influence on the biosynthesis of specific secondary metabolites, owing to its capacity to inhibit gene expression and repress enzyme activity [51].…”

Purification and Characterization of a Small Thermostable Protease from Streptomyces sp. CNXK100

“…The CNXK100 protease has been found to possess fibrinolytic activity, a characteristic documented in a few other studies involving proteases derived from S. radiopugnans VITSD8 ( Verma et al 2018 ), S. rubiginosus VITPSS1 ( Dhamodharan et al 2019 ), and Streptomyces sp. 214L–11 ( He et al 2023 ). Due to its stability towards pepsin, trypsin, chymotrypsin, and fibrinolytic activity, the CNXK100 protease holds potential applications in medications ( Bose et al 2019 ).…”

Purification and Characterization of a Novel Fibrinolytic Enzyme from Marine Bacterium Bacillus sp. S-3685 Isolated from the South China Sea

“…This enzyme bears resemblance to the one initially isolated from the Gobi radiation zone by our research group [ 31 ]. Undoubtedly, abnormal environments, such as the ocean’s high salinity, high pressure, and polar environment, offer promising prospects for future microbiology and biotechnology studies [ 32 ].…”