Screening Novel Biomarkers for Metabolic Syndrome by Profiling Human Plasma N-Glycans in Chinese Han and Croatian Populations

Abstract

N-glycans play an essential role in biological process and are associated with age, gender, and body mass parameters in Caucasian populations, whereas no study has been reported in Chinese populations. To investigate the correlation between N-glycan structures and metabolic syndrome (MetS) components, we conducted a population-based study in 212 Chinese Han individuals. The replication study was performed on 520 unrelated individuals from a Croatian island Korčula. The most prominent observation was the consistent positive correlations between different forms of triantennary glycans and negative correlations between glycans containing core-fucose with MetS components including BMI, SBP, DBP, and fasting plasma glucose (FPG) simultaneously. Significant differences in a number of N-glycan traits were also detected between normal and abnormal groups of BMI, BP, and FPG, respectively. In the multivariate analysis, the level of monosialylated glycans (structure loadings = -0.776) was the most correlated with the MetS related risk factors, especially with SBP (structure loadings = 0.907). Results presented here are showing that variations in the composition of the N-glycome in human plasma could represent the alternations of human metabolism and could be potential biomarkers of MetS.

The Association Between Glycosylation of Immunoglobulin G and Hypertension

“…The results from our previous study suggested that N- glycosylation of plasma proteins is affected by both age and sex, and that changes in the levels of glycan features can be associated with metabolic syndrome components including BMI, SBP, DBP, and fasting plasma glucose simultaneously. 31 The present data suggested that blood pressures are associated with individual variation in the extent of fucosylation, the degree of branching, levels of sialylation, and levels of glycosylation. Individual variation in IgG glycome composition is very high.…”

“…Based on our previously publication, in which plasma N -Glycan G0 is associated with SBP in Chinese Han and Croatian populations ( r  = 0.194 and 0.314, respectively), 31 the sample size of each group was estimated to be 538 using StatsToDo (http://www.statstodo.com/SSizCorr_Pgm.php) at the significance level of 0.05/23/2 and power of 90%.…”

“…Recently, we reported associations between plasma glycans and blood pressures in both Chinese and Croatian populations. 31 In this study, we focused on IgG glycans to eliminate the effects of varying concentrations of plasma proteins, which might complicate the interpretation of the changes observed in the total plasma glycome.…”

Human Plasma N-glycosylation as Analyzed by Matrix-Assisted Laser Desorption/Ionization-Fourier Transform Ion Cyclotron Resonance-MS Associates with Markers of Inflammation and Metabolic Health

“…Previous studies of suitable size (n Ͼ 200) have performed this to various degrees, finding plasma N-glycans to be highly associated with e.g. age, sex, inflammation, body mass index (BMI), cholesterol and lipid levels (17)(18)(19)(20)(21)(22)(23)(24). However, these studies have either been performed on single proteins, immunoglobulin G (IgG) being a particularly wellstudied example, or predominantly by methods of liquid chromatographic and electrophoretic separation (e.g.…”

Suboptimal health: a potential preventive instrument for non-communicable disease control and management

“…The SHSQ-25 has proved to be a reliable and valid instrument for measuring sub-health status in urban Chinese [2]. The progress of a combined genomics and glycomics study for screening biomarkers and exploring SHS as a preventive tool for non-communicable disease control and management will be presented [3]. …”