Screening methylation of DNA repair genes in the oral mucosa of chronic smokers

Carta, Celina Faig Lima; Alves, Mônica Ghislaine Oliveira; Barros, Patrícia Pimentel de; Campos, Melina; Scholz, Jaqueline; Jorge, Antônio Olavo Cardoso; Nunes, Fábio Daumas; Almeida, Janete Días

doi:10.1016/j.archoralbio.2018.04.017

Cited by 8 publications

(10 citation statements)

References 39 publications

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“…The table (1) was showed that there was a significant effect of smoking on methylation of MLH1 gene compare to nonsmoking group P > 0.001. This result agree with (8,19,20) .…”

Section: Resultssupporting

confidence: 89%

Tobacco Smoking as A Risk Factor in DNA Methylation of Repair Gene (MLH1) Using Cytobbrush from Lateral Border of the Tongue

Muayad Hashim Matloob,

Ameena Ryhan Diajil

2021

Indian Journal of Forensic Medicine & Toxicology

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Objective:The aim of this study was to evaluate the epigenetic effect in the process of oral carcinogenesis by screening the methylation of repair gene in chronic tobacco smokers.Material and Method: Study design: One hundred male volunteers, divided into two groups: the 1 st group consisted of 58 smokers, each consumed 20 cigarettes/day for at least 10 years; and the 2 nd group consisted of 48non-smokers who were consider as a control group.The samples were taking from lateral border of the tongue by exfoliative cytology, and the extracted DNA was treated with phenol-chloroform gDNA,Screening of methylation was done by Methyl Specific PCR (MSP).Results :-The results of this studyshowed significant effect of tobacco smoking in methylation of MLH1 gene in site 1in comparison to non-smoker group,(P > 0.05), with Odds ratio = 4.957 CI ().

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“…The table (1) was showed that there was a significant effect of smoking on methylation of MLH1 gene compare to nonsmoking group P > 0.001. This result agree with (8,19,20) .…”

Section: Resultssupporting

confidence: 89%

Tobacco Smoking as A Risk Factor in DNA Methylation of Repair Gene (MLH1) Using Cytobbrush from Lateral Border of the Tongue

Muayad Hashim Matloob,

Ameena Ryhan Diajil

2021

Indian Journal of Forensic Medicine & Toxicology

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“…Besides, other previous studies found an altered expression of these genes in smokers, a group that is at risk of developing carcinogenesis, and, likewise, methylation and a reduction of the gene expression were also observed. 16 Wagner et al 23 observed a high expression of hMSH2 and they suggested that this can indicate a greater activation of the induced genomic instability. Therefore, we conjecture that MSH2 overexpression in OL and OSCC seen in our cases represents genomic instability that induced gene activation.…”

Section: Discussionmentioning

confidence: 99%

MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma

Donís

González

Alves

et al. 2021

Applied Immunohistochemistry &Amp; Molecular Morphology

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“…Low expression of these genes is associated with reduced DNA repair capacity and development of malignant lesions (40). Additionally, previous studies have reported that reduced expression of these genes is correlated with smoking-induced methylation in smokers at risk of cancer (41). DSBs are mainly repaired by the HR and NHEJ pathways.…”

Section: Dna Damage Repair In Hnsccmentioning

confidence: 99%

“…related to smoking-induced methylation in smokers at risk for cancer (41) MLH2 high related to increased risk of OSCC, especially in patients with comorbidities (39) low related to reduced DNA repair capacity and the development of malignant lesions (40) related to smoking-induced methylation in smokers at risk for cancer (41) MSH3 low related to reduced DNA repair capacity and the development of malignant lesions(40)related to smoking-induced methylation in smokers at risk for cancer(41) …”

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confidence: 99%

Targeting DNA damage response as a potential therapeutic strategy for head and neck squamous cell carcinoma

Lei

He²,

Jiang³

et al. 2022

Front. Oncol.

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Cells experience both endogenous and exogenous DNA damage daily. To maintain genome integrity and suppress tumorigenesis, individuals have evolutionarily acquired a series of repair functions, termed DNA damage response (DDR), to repair DNA damage and ensure the accurate transmission of genetic information. Defects in DNA damage repair pathways may lead to various diseases, including tumors. Accumulating evidence suggests that alterations in DDR-related genes, such as somatic or germline mutations, single nucleotide polymorphisms (SNPs), and promoter methylation, are closely related to the occurrence, development, and treatment of head and neck squamous cell carcinoma (HNSCC). Despite recent advances in surgery combined with radiotherapy, chemotherapy, or immunotherapy, there has been no substantial improvement in the survival rate of patients with HNSCC. Therefore, targeting DNA repair pathways may be a promising treatment for HNSCC. In this review, we summarized the sources of DNA damage and DNA damage repair pathways. Further, the role of DNA damage repair pathways in the development of HNSCC and the application of small molecule inhibitors targeting these pathways in the treatment of HNSCC were focused.

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Screening methylation of DNA repair genes in the oral mucosa of chronic smokers

Cited by 8 publications

References 39 publications

Tobacco Smoking as A Risk Factor in DNA Methylation of Repair Gene (MLH1) Using Cytobbrush from Lateral Border of the Tongue

Tobacco Smoking as A Risk Factor in DNA Methylation of Repair Gene (MLH1) Using Cytobbrush from Lateral Border of the Tongue

MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma

Targeting DNA damage response as a potential therapeutic strategy for head and neck squamous cell carcinoma

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