Screening method for inherited disorders of purine and pyrimidine metabolism by capillary electrophoresis with reversed electroosmotic flow

Adam, Tomáš; Lochman, Pavel; Friedecký, David

doi:10.1016/s1570-0232(01)00591-8

Cited by 18 publications

(16 citation statements)

References 25 publications

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“…These methods are very important diagnostic tools for these disorders. In particular the report by Adam et al [105] is interesting because it describes a MEKC method with electroosmotic flow reversed by addition of a cationic surfactant (1 mM cetyltrimethylammonium bromide) and 0.44% tetrabutylammonium hydroxide-2-amino-2 methyl-1,3-propane diol to a background electrolyte consisting of 45 mM borate, 55 mM N-Tris(hydroxymethyl)methylglycine, 10 mM tartrate (pH 8.6). Using this method, they obtained highly reproducible and linear separation of a mixture of purines, pyrimidines, and urinary constituents with an average sensitivity of about 2.51 mM.…”

Section: Metabolites Of Purines Pyrimidines Nucleosides and Nucleomentioning

confidence: 99%

Micellar electrokinetic chromatographic and capillary zone electrophoretic methods for screening urinary biomarkers of human disorders: A critical review of the state‐of‐the‐art

et al. 2005

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Human urine plays a central role in clinical diagnostic being one of the most-frequently used body fluid for detection of biological markers. Samples from patients with different diseases display patterns of biomarkers that differ significantly from those obtained from healthy subjects. The availability of fast, reproducible, and easy-to-apply analytical techniques that would allow identification of a large number of these analytes is thus highly desiderable since they may provide detailed information about the progression of a pathological process. From among the variety of methods so far applied for the determination of urinary metabolites, capillary electrophoresis, both in the capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) modes, represents a robust and reliable analytical tool widely used in this area. The aim of the present article is to focus the interest of the reader on recent applications of MEKC and CZE in the field of urinary biomarkers and to discuss advantages and/or limitations of each mode.

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Section: Metabolites Of Purines Pyrimidines Nucleosides and Nucleomentioning

confidence: 99%

Micellar electrokinetic chromatographic and capillary zone electrophoretic methods for screening urinary biomarkers of human disorders: A critical review of the state‐of‐the‐art

et al. 2005

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“…Purines and pyrimidines play an important role as precursors for the DNA and RNA nucleotide biosynthesis. Modified purines and pyrimidines excreted into the urine have been suggested as potential biomarkers for the early detection of cancer [101,102].…”

Section: Metabolites and Drugsmentioning

confidence: 99%

CE – a multifunctional application for clinical diagnosis

et al. 2007

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CE has been used widely as an analytical tool with high separation power taking advantage of size, charge-to-size ratio, or isoelectric point of various analytes. In combination with detection methods, such as UV absorption, electrochemical detection, fluorescence, or mass spectrometry (MS), it allows the separation and detection of inorganic and organic ions, as well as complex compounds, such as polypeptides, nucleic acids, including PCR amplicons from viruses or bacteria. Recent interest in identification of biomarkers of diseases using body fluids leads to development of CE-MS techniques. These applications allowed identification of new potential biomarkers for clinical diagnosis and monitoring of therapeutic interventions. In this report, we present a technical overview of various CE techniques and discuss their applications in clinical medicine.

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“…Modified purines and pyrimidines excreted in urine have also been used as potential biomarkers for the early detection of cancer. Adam et al [49] and Friedecky et al [50] developed CE methods for the diagnosis of purine and pyrimidine inherited enzyme deficiencies. Characteristic abnormalities were detected in the electropherograms of urine samples from patients with purine and pyrimidine enzyme deficiencies.…”

Section: Metabolites Of Purines and Pyrimidinesmentioning

confidence: 99%

Progress in capillary electrophoresis of biomarkers and metabolites between 2002 and 2005

2006

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Biomarker discovery and metabolite research is a fast-growing and extremely important domain not only for the early detection of certain diseases but also for controlling its progress as well as in pharmaceutical investigations. For the analytical separation and identification, CE plays an indisputable role. Capillary systems enhancing different selectivity are applied and connected to different kind of detection systems. As the choice of buffer and its composition is responsible for a successful separation, special emphasis is put on solvent effects in this review. Altogether the most important capillary electrophoretic techniques applied for biomarker and metabolites analysis published between 2002 and 2005 are summarized and discussed.

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Screening method for inherited disorders of purine and pyrimidine metabolism by capillary electrophoresis with reversed electroosmotic flow

Cited by 18 publications

References 25 publications

Micellar electrokinetic chromatographic and capillary zone electrophoretic methods for screening urinary biomarkers of human disorders: A critical review of the state‐of‐the‐art

Micellar electrokinetic chromatographic and capillary zone electrophoretic methods for screening urinary biomarkers of human disorders: A critical review of the state‐of‐the‐art

CE – a multifunctional application for clinical diagnosis

Progress in capillary electrophoresis of biomarkers and metabolites between 2002 and 2005

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