Screening method for detection of hydrocarbon-oxidizing bacteria in oil-contaminated water and soil specimens

Petrovic, O.; Knežević, Petar; Majstorović, Jelena; Srdjan, Rončević

doi:10.1016/j.mimet.2008.03.012

Cited by 26 publications

(11 citation statements)

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“…Chikere et al (2009) reported the prevalence of Flavobacterium, Enterobacter, Norcardia and Acinetobacter in a hydrocarbon Table 3 also indicates that the crude oil degradative ability of the individual bacterial isolates was significant as evidenced by turbidity and emulsification of 1 ml of crude oil in 10 ml of Bushnell-Haas broth after 14 days incubation when compared with the test isolates on day zero incubation as presented in Figure 1. Bacteria that are capable of utilizing hydrocarbons as energy and carbon sources in broth culture have been shown to produce bioemulsifiers or bio-surfactants that assist in the transport of hydrocarbons into the cell via efficient uptake systems (Atlas and Philip, 2005;Olga et al, 2008;Satpute et al, 2010;Cho et al, 2011). Previous studies have demonstrated that the bacterial genera characterized in the present investigation contain known hydrocarbon utilizing species (Chaillan et al, 2004;Brito et al, 2006;Olga et al, 2008;Kadali et al, 2012).…”

Section: Resultsmentioning

confidence: 79%

“…Bacteria that are capable of utilizing hydrocarbons as energy and carbon sources in broth culture have been shown to produce bioemulsifiers or bio-surfactants that assist in the transport of hydrocarbons into the cell via efficient uptake systems (Atlas and Philip, 2005;Olga et al, 2008;Satpute et al, 2010;Cho et al, 2011). Previous studies have demonstrated that the bacterial genera characterized in the present investigation contain known hydrocarbon utilizing species (Chaillan et al, 2004;Brito et al, 2006;Olga et al, 2008;Kadali et al, 2012).…”

Section: Resultsmentioning

confidence: 79%

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Culture-dependent characterization of hydrocarbon utilizing bacteria in selected crude oil-impacted sites in Bodo, Ogoniland, Nigeria

Chikere

Ekwuabu²

2014

Afr. J. Environ. Sci. Technol.

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This investigation was carried out to characterize microbial communities in selected crude oil polluted sites in Bodo community, Gokana Local Government Area of Rivers State, Nigeria. Total heterotrophic bacterial counts ranged from 0.7 to 1.37x10 7 cfu/g and 0.2 to 5.9x10 6 cfu/ml while counts of hydrocarbon utilizing bacteria ranged from 0.1 to 8.0 x 10 6 and 0.2 to 7.5 x 10 5 cfu/ml for soil, sediment and water, respectively. Physiochemical parameters of all samples were determined. The ranges obtained were temperature 31-33°C, pH 7.5-8.2, conductivity 1134-7680 µs/cm, total nitrogen 792.4-886.3 mg/kg, nitrate 36.55-42.70 mg/kg, total organic carbon 2.06-2.18%, total petroleum hydrocarbon 1007-1104 mg/kg, vanadium 0.001-0.007 mg/kg, iron 3.772-4.889 mg/kg, chromium 52.40-66.20 mg/kg, nickel 40.02-41.62 mg/kg, lead 17.30-19.40 mg/kg and zinc 35.10-39.50 mg/kg for soil and sediments while water had total nitrogen 868 mg/l, nitrate 40.6 mg/l, total organic carbon 3.1 mg/l, total petroleum hydrocarbon 768 mg/l, nickel 39.2 mg/l, lead 17.3 mg/l and turbidity 250 NTU. Bacteria isolates characterized belonged to these genera Bacillus, Proteus, Pseudomonas, Flavobacterium, Corynebacterium, Serratia, Micrococcus, Klebsiella, Enterobacter and Azotobacter. The findings reveal that there is a high population of active indigenous hydrocarbon utilizing bacteria which can be monitored and enhanced to bring about bioremediation in the study area.

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Section: Resultsmentioning

confidence: 79%

Section: Resultsmentioning

confidence: 79%

Culture-dependent characterization of hydrocarbon utilizing bacteria in selected crude oil-impacted sites in Bodo, Ogoniland, Nigeria

Chikere

Ekwuabu²

2014

Afr. J. Environ. Sci. Technol.

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“…This indicated that different clay treatments could provide different environments to soil microorganisms at different Cd levels. The bacterial CFU, however, insignificantly correlated with the corresponding DHA value due to a possible discrepancy between the cultivable bacteria and total number of soil microorganisms [43]. DHA is an intracellular enzyme which takes part in the oxidative metabolism of soil microorganisms.…”

Section: Dehydrogenase Activity (Dha)mentioning

confidence: 96%

Heavy metal-immobilizing organoclay facilitates polycyclic aromatic hydrocarbon biodegradation in mixed-contaminated soil

Biswas

Sarkar

Mandal

et al. 2015

Journal of Hazardous Materials

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Soils contaminated with a mixture of heavy metals and polycyclic aromatic hydrocarbons (PAHs) pose toxic metal stress to native PAH-degrading microorganisms. Adsorbents such as clay and modified clay minerals can bind the metal and reduce its toxicity to microorganisms. However, in a mixed-contaminated soil, an adsorption process more specific to the metals without affecting the bioavailability of PAHs is desired for effective degradation. Furthermore, the adsorbent should enhance the viability of PAH-degrading microorganisms. A metal-immobilizing organoclay (Arquad(®) 2HT-75-bentonite treated with palmitic acid) (MIOC) able to reduce metal (cadmium (Cd)) toxicity and enhance PAH (phenanthrene) biodegradation was developed and characterized in this study. The MIOC differed considerably from the parent clay in terms of its ability to reduce metal toxicity (MIOC>unmodified bentonite>Arquad-bentonite). The MIOC variably increased the microbial count (10-43%) as well as activities (respiration 3-44%; enzymatic activities up to 68%), and simultaneously maintained phenanthrene in bioavailable form in a Cd-phenanthrene mixed-contaminated soil over a 21-day incubation period. This study may lead to a new MIOC-assisted bioremediation technique for PAHs in mixed-contaminated soils.

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“…The reduction of a lipophilic mediator such as DCPIP (blue to colorless) coupled with the formation of oxidized products showed that the biodegradation had been carried out by metabolically active cell growth, not by adsorption to cells associated with the water-carbon interface (Kubota et al, 2008). The respiratory reduction of tetrazolium salts is another criterion employed by many researchers (Olga et al, 2008; Pirôllo et al, 2008) to determine the dehydrogenase activity of hydrocarbonoclastic bacterial strains. Upon reduction of this salt, the color changed to red due to the formation of insoluble formazans by the production of superoxide radicals and electron transport in the bacterial respiratory chain (Haines et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Identification of Electrode Respiring, Hydrocarbonoclastic Bacterial Strain Stenotrophomonas maltophilia MK2 Highlights the Untapped Potential for Environmental Bioremediation

Venkidusamy

Megharaj

2016

Front. Microbiol.

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Electrode respiring bacteria (ERB) possess a great potential for many biotechnological applications such as microbial electrochemical remediation systems (MERS) because of their exoelectrogenic capabilities to degrade xenobiotic pollutants. Very few ERB have been isolated from MERS, those exhibited a bioremediation potential toward organic contaminants. Here we report once such bacterial strain, Stenotrophomonas maltophilia MK2, a facultative anaerobic bacterium isolated from a hydrocarbon fed MERS, showed a potent hydrocarbonoclastic behavior under aerobic and anaerobic environments. Distinct properties of the strain MK2 were anaerobic fermentation of the amino acids, electrode respiration, anaerobic nitrate reduction and the ability to metabolize n-alkane components (C8–C36) of petroleum hydrocarbons (PH) including the biomarkers, pristine and phytane. The characteristic of diazoic dye decolorization was used as a criterion for pre-screening the possible electrochemically active microbial candidates. Bioelectricity generation with concomitant dye decolorization in MERS showed that the strain is electrochemically active. In acetate fed microbial fuel cells (MFCs), maximum current density of 273 ± 8 mA/m2 (1000 Ω) was produced (power density 113 ± 7 mW/m2) by strain MK2 with a coulombic efficiency of 34.8%. Further, the presence of possible alkane hydroxylase genes (alkB and rubA) in the strain MK2 indicated that the genes involved in hydrocarbon degradation are of diverse origin. Such observations demonstrated the potential of facultative hydrocarbon degradation in contaminated environments. Identification of such a novel petrochemical hydrocarbon degrading ERB is likely to offer a new route to the sustainable bioremedial process of source zone contamination with simultaneous energy generation through MERS.

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Screening method for detection of hydrocarbon-oxidizing bacteria in oil-contaminated water and soil specimens

Cited by 26 publications

References 11 publications

Culture-dependent characterization of hydrocarbon utilizing bacteria in selected crude oil-impacted sites in Bodo, Ogoniland, Nigeria

Culture-dependent characterization of hydrocarbon utilizing bacteria in selected crude oil-impacted sites in Bodo, Ogoniland, Nigeria

Heavy metal-immobilizing organoclay facilitates polycyclic aromatic hydrocarbon biodegradation in mixed-contaminated soil

Identification of Electrode Respiring, Hydrocarbonoclastic Bacterial Strain Stenotrophomonas maltophilia MK2 Highlights the Untapped Potential for Environmental Bioremediation

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