Screening for zearalenone in corn by competitive direct enzyme-linked immunosorbent assay

Warner, Roscoe L.; Ram, B. P.; Hart, L. P.; Pestka, James J.

doi:10.1021/jf00070a031

Cited by 53 publications

(33 citation statements)

References 23 publications

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“…Besides the ELISA, possible sources of variability are sample spiking and assay extraction efficiency. These recovery values as well as the intra-and interassay coefficients of variation reported here are similar to others previously described for mycotoxins (Azcona et al, 1990;Dixon et al, 1987;Warner et al, 1986).…”

Section: Mouse Immunization and Hybridoma Productionsupporting

confidence: 92%

Production of monoclonal antibodies to the mycotoxins fumonisins B1, B2, and B3

Azcona-Olivera¹,

Abouzied²,

Plattner³

et al. 1992

J. Agric. Food Chem.

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Monoclonal antibodies were prepared against the fumonisins, a group of mycotoxins produced by Fusarium moniliforme. Splenic lymphocytes from mice immunized with a fumonisin B1-cholera toxin conjugate were fused with NS-1 myeloma cells, and six hybridomas were selected. A direct competitive ELISA was devised whereby fumonisin B1-peroxidase and free fumonisin B1 competed for antibody binding. The detection limit for fumonisin B1 in this assay was 50 ng/mL. Antibodies also cross-reacted with fumonisins BP and B3. Mean concentrations of fumonisins B1, BP, and B3 required to inhibit 50% antibody binding for the six clones were 630,1800, and 2300 ng/mL, respectively. When the antibodies were applied to the direct ELISA in spiked (5-25 pglg) feed, the average recovery was 103 % , with mean intra-and interassay coefficients of variation of 11 and 15%, respectively. These antibodies should find wide usage in the ELISA screening of fumonisins in foods, feeds, and tissues.

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Section: Mouse Immunization and Hybridoma Productionsupporting

confidence: 92%

Production of monoclonal antibodies to the mycotoxins fumonisins B1, B2, and B3

Azcona-Olivera¹,

Abouzied²,

Plattner³

et al. 1992

J. Agric. Food Chem.

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“…TLC is inexpensive and robust, but not very sensitive and selective [9,10]. In contrast, ELISA and RIA offer high sensitivity [11], but, due to cross-reactivities, differentiation between the analytes is not possible and unambiguous analyte identification has to be performed by complementary methods. GC in combination with mass spectrometry (GC-MS) has been frequently used in this field, including for the simultaneous determination of all 5 analytes plus zearelanone (ZAN) [12][13][14], which was reported to be a possible metabolite due to oxidation [ 15].…”

Section: Introductionmentioning

confidence: 99%

Determination of zeranol, taleranol, zearalenone, α- and β-zearalenol in urine and tissue by high-performance liquid chromatography-tandem mass spectrometry

2000

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Key WordsColumn liquid chromatography Tandem mass spectrometry Zeranol, zearalenone and zearalenol SummaryAn LC-MS/MS method has been developed for the sensitive determination of the anabolic compound zeranol, its epimer taleranol and the mycotoxins zearalenone, azearalenol and fl-zearalenol in animal urine and meat samples. Sample preparation included extraction of meat samples and enzymatic digest of urine samples followed by solid phase extraction with RP-18 columns for sample clean-up. Mass spectrometric determination was carried out with an atmospheric pressure chemical ionisation interface (APCI) in the multi-reaction monitoring mode (MRM). Using the negative ion mode, a limit of detection between 0.1 and 0.5 ppb and a limit of determination between 0.5 and 1 ppb could be achieved. With zearalanone (ZAN) as internal standard, a linear range between 0.5 (1.0) and 100ppb in urine samples (cow, pig) and between 1 and 100 ppb in meat samples (cow, calf, pig) could be established. Depending on the biological matrix and analyte, standard deviations were below 8.5 %, with average recovery rates around 86 % to 102 % in spiked samples. The usefulness of the method developed was demonstrated via several contaminated cow and pig urine samples. the mycotoxins zearalenone (ZON) and e-zearalenol (7-ZOL) that can be carried over from contaminated feedstuffto animals [2][3][4]. ZON, e-ZOL and its epimer/%zearalenol (fl-ZOL) are produced by several Fusarium species that colonize grains [5]. High amounts can be found on maize, wheat, barley and oats. All three compounds exhibit distinct HC

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“…In recent years, efforts devoted to the creation of rapid, selective, and comparatively inexpensive zearalenone tests led to the development of several variants of the toxin determination by ELISA [21,22]. These analyses are based on the zearalenone-specific monoclonal and polyclonal antibodies and can be used (with certain limitations) for toxin detection in various stages of raw material processing.…”

Section: Deoxynivalenolmentioning

confidence: 99%