Screening for Wart Virus Infection in Normal and Abnormal Cervices by Dna Hybridisation of Cervical Scrapes

Wickenden, Colin; Malcolm, Alan D. B.; Steele, Annette; Coleman, D. V.

doi:10.1016/s0140-6736(85)91963-4

Cited by 114 publications

(30 citation statements)

References 14 publications

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“…studies in which the histology or cytology of the lesion was specified and DNA aUnless otherwise noted, figures are for type 16 only; bType 18 only; cTypes 16 and 18 combined; dTwo control groups were used, one for women with inflammatory cervical lesions, the other of women with normal cervical cytology; eTwo control groups were used, one from a venereal disease clinic and the other from a family planning clinic. 'Durst et al, 1983;2Boshart et al, 1984;3De Villiers et al, 1986;4Wagner et al, 1984; 'Schneider et al, 1985;6Schneider et al, 1987;7Scholl et al, 1985;8McCance et al, 1985;9Meanwell et al, 1987;I'Toon et al, 1986; ' 'Wickenden et al, 1985;'2Cox et al, 1986;'3Lorincz et al, 1986;'4Fukushima et al, 1985;"Crum et al, 1986;'6Prakash et al, 1985;17Reeves et al, 1987; '8Reeves (pers. comm).…”

Section: Hpv Prevalence Surveysmentioning

confidence: 99%

“…In some papers which do, the subjects come from a limited number of clinics or hospitals, but it is still not clear whether any further selection has taken place (Cox et al, 1986;Lorincz et al, 1986;Macnab et al, 1986;Meanwell et al, 1987;Schneider et al, 1987;Scholl et al, 1985;Wagner et al, 1984;Wickenden et al, 1985). Information on the source of cases is important in the choice of the control group.…”

Section: Hpv Prevalence Surveysmentioning

confidence: 99%

See 1 more Smart Citation

Does human papillomavirus cause cervical cancer? The state of the epidemiological evidence

Muñóz

Bosch²,

Kaldor³

1988

Br J Cancer

188

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Summary The human papillomavirus has emerged over the past decade as the leading candidate to be the sexually transmitted aetiological factor in cervical cancer. Although it appears that papillomavirus types 16 and 18 are associated with a higher risk of advanced cervical neoplasia, most of the evidence comes from studies which do not satisfy basic epidemiological requirements, and are therefore difficult to interpret. The most significant problems are the small sample size, potentially biased selection of study subjects, the difficulties in cytologically distinguishing precancerous lesions from papilloma infection of the cervix, the unknown specificity and sensitivity of the various hybridisation methods for determining papillomavirus infection status, and the statistical analyses and presentation of results. On the basis of the existing studies, one is forced to conclude that, while experimental data suggest an oncogenic potential for HPV, the epidemiological evidence implicating it as a cause of cervical neoplasia is still rather limited.

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Section: Hpv Prevalence Surveysmentioning

confidence: 99%

Section: Hpv Prevalence Surveysmentioning

confidence: 99%

Does human papillomavirus cause cervical cancer? The state of the epidemiological evidence

Muñóz

Bosch²,

Kaldor³

1988

Br J Cancer

188

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“…The currently used assays for the detection of HPV-specific nucleic acids rely either on the Southern blot or on one of the rapid blot procedures (57). Despite high levels of homology at the nucleic acid level, it has been possible to distinguish between HPV types using stringent hybridization conditions (58).…”

Section: Human Papillomavirus (Hpv)mentioning

confidence: 99%

“…Despite high levels of homology at the nucleic acid level, it has been possible to distinguish between HPV types using stringent hybridization conditions (58). The specimen to be tested can take the form either of a punch biopsy or a cervical scrape; the scrape is a particularly effective way of obtaining a sample because it is noninvasive and there are no ethical limitations in obtaining control material from normal individuals (57).…”

Section: Human Papillomavirus (Hpv)mentioning

confidence: 99%

Nucleic acid analysis by sandwich hybridization

Nicholls

Malcolm

1989

Clinical Laboratory Analysis

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P e t e r J o h n N i c h o l l s . A T h e s i s S u b m it t e d f o r t h e D e g r e e o f D o c t o r o f P h i lo s o p h y o f t h e U n i v e r s i t y o f L o n d o n . C h a r i n g C r o s s a n d W e s t m in s t e r M e d i c a l S c h o o l.M a r c h , 1 9 8 9 . 2 A b s t r a c t . T h e n u c l e i c a c i d -b a s e d t e s t s c u r r e n t l y u s e d f o r t h e d e t e c t io n o f i n h e r i t e d a n d i n f e c t i o u s d i s e a s e a r e s e n s i t i v e a n d s p e c i f i c , b u t n o t a lw a y s r e p r o d u c ib le , b e c a u s e o f p r o c e d u r a l c o m p l e x i t i e s ; t h i s i n t u r n m eans t h a t t h e y a r e n o t a m e n a b le t o a u t o m a t io n . S u c h t e s t s a r e n o t c o s t -e f f e c t i v e , a r e l i m i t e d i n t h e num ber o f s a m p le s w h ic h c a n b e p r o c e s s e d a t o n c e , a n d m o s t com m only r e l y o n u n s t a b le i s o t o p i c a l l y l a b e l l e d p r o b e s , w h ic h a r e h a z a r d o u s t o h a n d le a n d d i s p o s e o f . A n u c l e i c a c i d s a n d w ic h h y b r i d i z a t i o n a s s a y h a s b e e n d e v e lo p e d , i n i t i a l l y u s i n g c lo n e d hum an p a p i l l o m a v i r u s (H PV) s e q u e n c e s a s a m o d e l, a n d s u b s e q u e n t ly f o r t h e d e t e c t io n a n d t y p i n g o f H PV i n f e c t i o n i n c e r v i c a l s c r a p e s . T h e i m p l i c a t i o n s o f c e r v i c a l HPV i n f e c t i o n a r e o f c l i n i c a l s i g n i f i c a n c e , b e c a u s e o f t h e r e l a t i v e l y r e c e n t a c c u m u la t io n o f e v id e n c e l i n k i n g s p e c i f i c H PV t y p e s w i t h t h e a e t i o l o g y o f c e r v i c a l c a n c e r . C o n s i d e r i n g t h a t v i r a l t y p e c o u ld h a v e a g r e a t i n f l u e n c e o n t h e p r o g n o s i s o f i n f e c t i o n , a n d t h a t im m u n o lo g ic a l a s s a y s a r e n o t c a p a b leo f d i f f e r e n t i a t i n g b e tw e e n t y p e s , t h e s a n d w ic h h y b r i d i z a t i o n t e c h n iq u e , w h ic h c o u ld h a n d le l a r g e n um bers o f s a m p le s , w o u ld b e a n i n v a l u a b l e t o o l f o r im p r o v in g t h e e f f i c a c y o f t h e c e r v i c a l s c r e e n i n g p rogram m e. T o Mun a n d D ad, w ith a l l n y lo v e . T h e a s s a y r e q u i r e s tw o d i s t i n c t , n o n -o v e r la p p i n g ENA fr a g m e n t s , d e r iv e d fr o m a d j a c e n t p o s i t i o n s o n t h e v i r a l genom e. One f r a g m e n t (A ) i s c o v a l e n t l y a t t a c h e d t o a S-T h a n k s f o r m a k in g t h i s p o s s i b l e - 5T h e kncw n i s f i n i t e , t h e unknow n i n f i n i t e ; i n t e l l e c t u a l l y we s t a n d o n a n i s l e t i n t h e m id s t o f a n i l l i m i t a b l e o c e a n o f i n e x p l i c a b i l i t y . O u r b u s i n e s s i n e v e r y g e n e r a t io n i s t o r e c l a i m a l i t t l e m ore la n d .T .H . H u x le y , 1 8 8 7 . F i r s t a n d f o r e m o s t ,...

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“…The detection and typing of cervical HPV infections generally have been performed on DNA extracted from fresh biopsies or cell scrapes by Southern or dot blot hybridisation [Durst et al, 1983;Boshart et al, 1984;Wickenden et al, 1985;Kulski et al, 19871 and on fresh cell scrapes by filter in situ hybridisation (FISH) Wagner et al, 1984;Schneider et al, 1985;McCance et al, 1986;Demeter et al, 19871. Cell scrapes are easier to obtain than biopsies and are an important source of fresh material for analysis of HPV cervical infections and prospective epidemiological studies on the role of HPV in the development of CIN and squamous cell carcinoma.…”

Section: Introductionmentioning

confidence: 99%

Detection of human papillomavirus DNA in cell scrapes and formalin‐fixed, paraffin‐embedded tissue of the uterine cervix by filter in situ hybridisation

Demeter

Kulski

Rakoczy

et al. 1988

Journal of Medical Virology

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Filter in situ hybridisation (FISH) was used to detect the presence of DNA of human papillomavirus (HPV) types 6/11 or 16/18 in cell scrapes (CYTOFISH) and formalin-fixed, paraffin-embedded biopsies (HISTOFISH) taken from the uterine cervices of 19 women. Paraffin tissue sections collected for HISTOFISH were either digested with pepsin or lysed with alkali/Triton X-100. The digest or lysate of the tissue sections and cell scrapes were applied to nylon or nitrocellulose membranes for nucleic acid hybridisation using 32P-labeled HPV-DNA probes. CYTOFISH and HISTOFISH were compared directly by taking samples for each method from the cervices of the same women. Of 19 women examined by colposcopy, cytology, and histology, eight were assessed as normal and 11 had evidence of a cervical disorder and/or the presence of HPV infection. Whereas no HPV-DNA was detected in the normal cases, the presence of HPV-DNAs was detected by both CYTOFISH and HISTOFISH in 11 cases with histological evidence of HPV infection and/or dysplasia. In these HPV positive cases, eight contained HPV 16/18, two HPV 6/11, and one a mixed infection of HPV 6/11/16/18. The high correlation between the results of CYTOFISH and HISTOFISH shows that formalin-fixed, paraffin-embedded cervical biopsies are suitable specimens for the detection and typing of HPV-DNA by FISH. Both CYTOFISH and HISTOFISH should facilitate studies on the prevalence and distribution of HPVs and their association with neoplasia.

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Screening for Wart Virus Infection in Normal and Abnormal Cervices by Dna Hybridisation of Cervical Scrapes

Cited by 114 publications

References 14 publications

Does human papillomavirus cause cervical cancer? The state of the epidemiological evidence

Does human papillomavirus cause cervical cancer? The state of the epidemiological evidence

Nucleic acid analysis by sandwich hybridization

Detection of human papillomavirus DNA in cell scrapes and formalin‐fixed, paraffin‐embedded tissue of the uterine cervix by filter in situ hybridisation

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