Screening for the presence of biosynthetic genes for antimicrobial lipopeptides in natural isolates of Bacillus sp.

Abstract

A collection of 205 natural isolates of Bacillus was tested for the presence of genes for biosynthesis of antimicrobial lipopeptides, iturin, surfactin, fengycin and bacillomycin D. For the detection of iturin producers by PCR screening, we used forward ITUP1-F and reverse ITUP2-R primers which are capable of detecting a 2-kb region that includes the intergenic sequence between the ituA and ituB genes. A 675-bp fragment from the gene sfp from B. subtilis encoding 4’-phosphopantetheinyl transferase involved in the biosynthesis of surfactin was targeted for amplification by using primers P17 and P18. Other two pairs of primers were BACC1F and BACC1R for bacillomycin D and FEND1F and FEND1R for potential fengycin producers, respectively. The results of the screening showed that the majority of tested strains had more than one biosynthetic operon, since 81% possessed the genes for bacillomycin D production, 54% for surfactin, 38% for iturin and 25% for fengycin production. [Projekat Ministarstva nauke Republike Srbije, br. 173026

The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains

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Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains

“…The strains used in this study are part of previously established and characterized collections (Stanković et al . ; Nikolić et al . ).…”

Microbial Biosurfactant for Hydrocarbons and Heavy Metals Bioremediation

“…(Hong et al 2005;Chang et al 2008;Jayabarath et al 2009;Mani et al 2011;Milena et al 2012) and Bacillus sp. (Christova et al 2004;Priya and Usharani 2009;Kosaric 2001;Stankovic et al 2012;Pradhan et al 2013Pradhan et al , 2014a. Other reported bacteria are Rhodococcus (Kosaric 2001), Corynebacterium (Thavasi et al 2010), Sphingomonas (Chang et al 2008), Serratia (Anyanwu et al 2011), and Lactococcus (Rodrigues et al 2006).…”