Screening for resistance to big-vein disease of lettuce (Lactuca sativa)

Bos, L.; Huijberts, N.

doi:10.1016/0261-2194(90)90135-t

Cited by 28 publications

(16 citation statements)

References 11 publications

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“…However no L. sativa genotype has been identified as immune to big-vein (Bos and Huijberts, 1990;Ryder and Robinson, 1995) and only partial resistance is available in cultivated lettuce, characteristic expressed as the delayed appearance of symptoms combined with a reduced number of symptomatic plants at market maturity (Ryder and Robinson, 1995;Hayes and Ryder, 2007). Traditional breeding has developed cultivars with some levels of resistance such as Pacific and Pavane (Ryder and Robinson, 1991;Compositdb, 2004), but resistance levels of cultivars released to date are not as high (Kawazu et al, 2010).…”

Section: Bvd Is Associated With Two Viral Agents Mirafiori Lettuce Bmentioning

confidence: 99%

Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease

Araya

Peña

Salazar

et al. 2011

Chilean J. Agric. Res.

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Big-vein disease (BVD) is a widespread and economically damaging disease in lettuce (Lactuca sativa L.). Typical symptoms are chlorotic clearing around leaf veins, leaf deformations, and impaired head development. In this research, we studied the relationship between symptom intensity and protein and viral RNA accumulation in infected plants. Naturally infected lettuce plants, from the field or greenhouse, were classified according to their symptomatology: mild, moderate, severe, and symptomless. Coat protein accumulation was evaluated by a double antibody sandwich/enzyme-linked immunosorbent assay (DAS-ELISA), and RNA levels were studied by semiquantitative reverse transcription polymerase chain reaction (RT-PCR) and quantitative RT-PCR. Virus coat protein accumulation did not differ for the two viruses associated with this disease among lettuce plants showing different symptom severity. Similarly, abundance of Mirafiori lettuce big-vein virus (MLBVV)-RNA3 or Lettuce big-vein associated virus (LBVaV)-RNA-2 were not different (P > 0.05) for diverse big vein disease severity rating scales. This suggests that symptom severity expressed by big-vein diseased lettuce plants did not necessarily reflect the accumulation of viruses associated with this disease in the host. Therefore, lettuce plants showing mild symptoms of BVD do not necessarily present lower virus levels than plants showing more severe symptomatology.

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Section: Bvd Is Associated With Two Viral Agents Mirafiori Lettuce Bmentioning

confidence: 99%

Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease

Araya

Peña

Salazar

et al. 2011

Chilean J. Agric. Res.

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“…(1985) pointed to a possible difference between the agents of LRN and LBV, but their attempts to mechanically transmit and purify the LRN agent failed. Parallel and further to our earlier studies Bos and Huijberts, 1990) and those of Vetten et al (1987) on LBV, we also tried to identify the cause of LRN and to study its possible relationships with LBV 'virus' (LBVV), which had been found related to, but different from, tobacco stunt virus (TStV) described in Japan (Vetten et al, 1987;Huijberts et al, 1990). Although associated with virus-like particles, the very labile agents of LBV and of the possibly related freesia leaf necrosis (Bouwen, 1994) remain shrouded in mystery.…”

Section: Introductionmentioning

confidence: 62%

Lettuce ring necrosis, caused by a chytrid-borne agent distinct from lettuce big-vein ‘virus’

Bos¹,

Huijberts²

1996

Eur J Plant Pathol

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Ring necrosis is a serious disease of lettuce (Lactuca sativa) with often coalescing necrotic rings and ring-like patterns on middle leaves of plants or groups of plants in glasshouses during winter. Affected leaves may decay and plants rapidly become unmarketable. The disease was shown to be soil-bome and transmitted by the zoospores of Olpidium brassicae. Symptoms in lettuce do not appear before seven weeks after inoculation via the soil. Additives to the inoculum and chilling of source leaves, inoculum buffer and utensils enabled mechanical transmission of a pathogenic agent to Chenopodium quinoa, C. amaranticolor, Nicotiana benthamiana, N. clevelandii, N. hesperis, and N. occidentalis but not to lettuce. The Chenopodium spp. reacted with local lesions, infection was symptomless in N. clevelandii and mostly so in N. benthamiana, but N. hesperis and N. occidentalis reacted with leaf spotting and plant stunting. With zoospores of an originally pathogen-free fungus culture further cultivated on the roots of cuttings from sap-inoculated plants of N. clevelandii and N. occidentalis, the agent could be transferred back to lettuce and the symptoms of ring necrosis be reproduced. The agent biologically resembles those of lettuce big-vein (LBV) and freesia leaf necrosis and the tobacco stunt virus. In lettuce it often occurs together with LBV 'virus' but differs in longer incubation period, type of symptoms and symptom appearance only during winter. It could be separated from a mixture with LBV 'virus' by serial transfer always selecting plants without LBV symptoms. So far cultural hygiene, including soil disinfection addressing the vector, is the main means of control.

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“…Dahm (1993) added zoospores to plants, grown in sand, whereas Adams et al (1986), in a test for resistance to P. graminis and BaYMV, transplanted seedlings after inoculation. Bos and Huijberts (1990), in a test for resistance to lettuce big vein virus, added zoospores of Olpidium brassicae directly into the pots with soil. In all situations, susceptible and resistant cultivars could be distinguished.…”

Section: Discussionmentioning

confidence: 99%

Use of zoospores of Polymyxa betae in screening beet seedlings for resistance to beet necrotic yellow vein virus

Paul¹,

Henken²,

Scholten³

et al. 1993

Neth. J. Pl. Path.

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A system to culture viruliferous Polymyxa betae and to produce zoospores is described. The zoospores were used for inoculation of beet seedlings, grown in nutrient solution, in tests for resistance to beet necrotic yellow vein virus (BNYVV). On most occasions in a time course experiment, and with various zoospore cultures, the partially resistant cultivar Rima and the accession Holly-1-4 had virus concentrations similar to the susceptible cultivar Regina, but the virus concentration in Beta vulgaris ssp. maritima accession WB42 was significantly lower (P < 0.05). 'Regina' could be distinguished from various resistant accessions by a significantly higher virus concentration (P < 0.05) shortly after inoculation, or after transplanting the seedlings from the nutrient solution into sand. Results of screening for resistance to BNYVV, using zoospores for inoculation, did not correspond with results of a test in which infested soil was used.Tests in which seedlings are grown in nutrient solution and inoculated with zoospores are suitable for the detection of accessions with a high level of resistance to BNYVV. To obtain virus infection in all plants, the optimal density of the zoospore suspension should first be determined and plants should be assayed shortly after inoculation.

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Screening for resistance to big-vein disease of lettuce (Lactuca sativa)

Cited by 28 publications

References 11 publications

Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease

Symptom severity and viral protein or RNA accumulation in lettuce affected by big-vein disease

Lettuce ring necrosis, caused by a chytrid-borne agent distinct from lettuce big-vein ‘virus’

Use of zoospores of Polymyxa betae in screening beet seedlings for resistance to beet necrotic yellow vein virus

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