Screening for periodontopathogenic bacteria in severe chronic periodontitis in a Moroccan population

Benrachadi, Latifa; Bouziane, Amal; Azziman, Z.; Bouziane-Ouartini, F.; Ennibi, Oum Keltoum

doi:10.1016/j.medmal.2012.10.003

Cited by 15 publications

(19 citation statements)

References 23 publications

(32 reference statements)

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“…In our study, the increased prevalence of P. gingivalis associated with other periodontal bacteria correlated with initial clinical examination allowed us to diagnose most patients with severe chronic periodontitis. Some authors consider the presence of A. actinomycetemcomitans in subgingival plaque samples as a marker for the diagnosis of aggressive periodontitis [17], while others consider that the microbiological profile of patients with severe chronic periodontitis is similar to those with aggressive periodontitis [18,19]. A quantitative microbiological analysis for the ratio of A.a compared to other periodontal bacterial pathogens could help in the differential diagnosis between the two pathologic entities [20].…”

Section: Resultsmentioning

confidence: 99%

“…The most important role of all the factors involved in developing this disease is played by the microbial factor, all the other ones being favoring or predisposing factors. Some bacterial species in the subgingival plaque, periodontal pathogens, interact with host tissues and cells and lead to the release of cytokines and other mediators of inflammation, resulting in the destruction of periodontal structures [1][2][3]. The red complex, consisting of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia, is heavily involved in the destructive stages of certain types of periodontal disease [4][5][6][7].…”

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confidence: 99%

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The Use of Polymerase Chain Reaction (PCR) for Indentifying Periodontopathogenic Bacteria - therapeutic Implications in Periodontal Disease

Lazăr¹,

Bică²,

Martha³

et al. 2017

Rev. Chim.

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Polymerase chain reaction (PCR) shows a high specificity and allows us to identify pathogenic periodontal bacteria. We chose 45 patients wich were divided into three groups with various types of treatment: (I) - SRP; (II) - SRP, followed by topical application of antiseptics (SRP + local); (III) - SRP followed by systemic administration of antimicrobial agents (SRP + systemic). We collected samples from the initial time (TO) and one month after the treatment (T1) for each patient. For the microbiological assessment of periodontal therapy, we analyzed 90 subgingival plaque samples using PCR technique which provides qualitative data on five periodontopathogenic bacteria species: A. actinomycetemcomitans, P.gingivalis, P.intermedia, T.forsythia, and T.denticola. The treatment was followed by a qualitative change of the bacteria detected previously in a different ratio depending on the treatment. We found the inefficiency of mechanical treatment regarding the reduction of periodontal bacteria in patients belonging to group I, an improvement in the results of group II, while the treatment in group III proved to be the most effective . In patients detected A.a+ and/ or P.g+ a systemic antibiotic treatment is required because these periodontal bacteria penetrate the tissue and mucosal surfaces of the oral cavity.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

The Use of Polymerase Chain Reaction (PCR) for Indentifying Periodontopathogenic Bacteria - therapeutic Implications in Periodontal Disease

Lazăr¹,

Bică²,

Martha³

et al. 2017

Rev. Chim.

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“…In ruminants, Tannerella forsythia has been identified in sheep (McCourtie et al 1989, Duncan et al 2003, Agostinho 2017, cattle (Borsanelli 2017), and goats (Campello 2017) with periodontitis. This microorganism has been frequently detected in human patients with periodontitis (Cortelli et al 2005, Foschi et al 2005, Feng & Weinberg 2006, Yoo et al 2007, Gaetti-Jardim et al 2010, Benrachadi et al 2012 and, initially, it was believed to be a relatively uncommon species found in the subgingival sulcus biofilm. Presently, it is known that its levels are strongly associated with increased depth of the periodontal pocket.…”

Section: Discussionmentioning

confidence: 99%

“…Healthy sites show few or none spirochetes and sites with gingival inflammation but without attachment loss, exhibit low to moderate indexes, whereas deep periodontal pockets harbor a large number of these bacteria (Socransky & Haffajee 2010). T. denticola is considered one of the major periodontopathogens in humans (Mayanagi et al 2004, Foschi et al 2005, Feng & Weinberg 2006, Gaetti-Jardim et al 2010, Benrachadi et al 2012, and it has also been identified in periodontal lesions in bovines, sheep and goats (Borsanelli et al 2015a, 2016, Agostinho 2017, Borsanelli 2017, Campello 2017.…”

Section: Discussionmentioning

confidence: 99%

Subgingival bacterial microbiota associated with ovine periodontitis

et al. 2019

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Periodontitis is an inflammatory response in a susceptible host caused by complex microbiota, predominantly composed of Gram-negative anaerobic bacteria. Aiming to characterize the subgingival bacterial microbiota associated with ovine periodontitis, polymerase chain reaction (PCR) was performed in subgingival periodontal pocket samples of 14 sheep with severe periodontitis and in subgingival sulcus biofilm of 14 periodontally healthy sheep in search mainly of Gram-negative and Gram-positive microorganisms considered important periodontopathogens. The most prevalent bacteria in the sheep with periodontal lesions were Tannerella forsythia (78.6%), Treponema denticola (78.6%), Fusobacterium nucleatum (64.3%), and Porphyromonas gingivalis (50%), whereas in the healthy sheep, F. nucleatum (42.8%) was the most often detected bacterium. Statistically significant differences were observed for Campylobacter rectus, Enterococcus faecium, Prevotella nigrescens, T. forsythia, and T. denticola (p<0.05) in the sheep with periodontitis in the comparison between groups. Aggregatibacter actinomycetemcomitans, Enterococcus faecalis, and Porphyromonas gulae were not detected in any of the samples analyzed. In conclusion, C. rectus, E. faecium, P. nigrescens, T. forsythia, and T. denticola were associated with severe lesions caused by ovine periodontitis, and F. nucleatum was the most prevalent microorganism in the subgengival sulcus biofilm of healthy sheep.

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“…The authors of various studies on P. gingivalis have reported that these pathogens are able to pollute soft tissues and flee the surgical debridement of periodontal lesions (28,29). Many epidemiological studies report a positive association between the presence of P. gingivalis and periodontal diseases (7,25,(30)(31)(32)(33)(34)(35)(36), whereas in some studies, no association was found between the presence of P. gingivalis and periodontal diseases (20,(37)(38)(39)(40)(41). This has also been shown by Kumar et al (42).…”

Section: Discussionmentioning

confidence: 99%

Study of Porphyromonas gingivalis in periodontal diseases: A systematic review and meta-analysis

Rafiei

Kiani

Sayehmiri

et al. 2017

Med. J. Islam. Republic Iran

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Background: The mouth cavity hosts various types of anaerobic bacteria including Porphyromonas gingivalis, which causes periodontal inflammatory diseases. P. gingivalis is a gram-negative oral anaerobe and is considered as a main etiological factor in periodontal diseases. Several studies have reported a relationship between P. gingivalis in individuals with periodontal diseases and a critical role of this bacterium in the pathogenesis of periodontal diseases. The present study aimed at estimating this probability using a meta-analysis.Methods: We searched several databases including PubMed, Scopus, Google Scholar, and Web of Science to identify case-control studies addressing the relationship between P. gingivalis with periodontal diseases. A total of 49 reports published from different countries from 1993 to 2014 were included in this study. I² (heterogeneity index) statistics were calculated to examine heterogeneity. Data were analyzed using STATA Version 11.Results: After a detailed analysis of the selected articles, 49 case-control studies with 5924 individuals fulfilled the inclusion criteria for the meta-analysis. The healthy controls included 2600 healthy individuals with a Mean±SD age of 36.56±7.45 years. The periodontal diseases group included 3356 patients with a mean age of 43.62±8.35 years. There was a statistically significant difference between P. gingivalis in periodontal patients and healthy controls; 9.24 (95% CI: 5.78 to 14.77; P = 0.000). In the other word, there was a significant relationship between the presence of P. gingivalis and periodontal diseases.Conclusion: Analyzing the results of the present study, we found a strong association between the presence of P. gingivalis and periodontal diseases. This result suggests that another research is needed to further assess this subject.

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Screening for periodontopathogenic bacteria in severe chronic periodontitis in a Moroccan population

Cited by 15 publications

References 23 publications

The Use of Polymerase Chain Reaction (PCR) for Indentifying Periodontopathogenic Bacteria - therapeutic Implications in Periodontal Disease

The Use of Polymerase Chain Reaction (PCR) for Indentifying Periodontopathogenic Bacteria - therapeutic Implications in Periodontal Disease

Subgingival bacterial microbiota associated with ovine periodontitis

Study of Porphyromonas gingivalis in periodontal diseases: A systematic review and meta-analysis

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