Screening for MiRNAs Related to Laryngeal Squamous Carcinoma Stem Cell Radiation

Huang, Chunhui; Zhu, Ying; Duan, Guangliang; Yao, Jing; Li, Zhaoyang; Li, Da; Wang, Qingqing

doi:10.7314/apjcp.2013.14.8.4533

Cited by 18 publications

(21 citation statements)

References 24 publications

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“…Previous studies validate that DNA methylation is associated with hypoxia which will affect the activity of TET enzyme, DNMT1 [42,43]. The abnormal miRNA expression is related to tumor microenvironment, such as hypoxia [44,45]. In our study, PCR showed that miR-410 was increased after 5-Aza treatment, and MSP results indicated that miR-410 was hypermethylated in T47D and MCF-7 cells, respectively.…”

Section: Discussionmentioning

confidence: 48%

MiR-410 Acts as a Tumor Suppressor in Estrogen Receptor-Positive Breast Cancer Cells by Directly Targeting ERLIN2 via the ERS Pathway

Guo

et al. 2018

Cell Physiol Biochem

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Background/Aims: Endoplasmic reticulum lipid raft-associated 2 (ERLIN2) is reported to be overexpressed in human breast cancer cells and plays an important role in cell proliferation. MicroRNAs (miRNAs) act as post-transcriptional regulators of gene expression and are involved in the development of multiple malignancies, including breast cancer. However, the molecular mechanism of the aberrant ERLIN2 expression in human breast cancer remains poorly understood. Methods: MiR-410 expression level was analyzed using Real-time PCR, and ERLIN2 expression was analyzed using Western blot, Real-time PCR and immunohistochemical staining. The effect of miR-410 on ERLIN2 3’UTR intensity was performed using a luciferase assay. Cell proliferation was analyzed using CCK-8 and colony formation assay, together with an Annexin V-PE/7-AAD kit for cell apoptosis assay. Cell migration and invasion was detected using a Transwell migration and invasion assay. Methylation specific PCR was used to examine whether miR-410 promoter was demethylated. Results: In this study, we validated that ERLIN2 was a direct target of miR-410 and miR-410 suppressed ERLIN2 expression at the post-transcriptional level. Importantly, the regulation of ERLIN2 by miR-410 was estrogen receptor (ER) dependent. Functional studies demonstrated that miR-410 inhibited breast cancer cell proliferation, migration and invasion, but promoted cell apoptosis. However, inhibition of miR-410 resulted in opposite effects. A xenograft nude mouse model further confirmed that miR-410 suppressed breast tumor growth. In addition, miR-410 modulated the expression levels of epithelial-mesenchymal transition (EMT)-related genes. ERLIN2 knockdown suppressed cell proliferation, migration and invasion, as well as EMT. ERLIN2 overexpression can restore the cell proliferation, migration and invasion that were inhibited by miR-410. Furthermore, our data demonstrated that miR-410 inhibition suppressed the expression of endoplasmic reticulum-stress (ERS)-related genes, while ERLIN2 knockdown abrogated the effects of miR-410 inhibitor. Finally, we showed that miR-410 was downregulated in human ER-positive breast cancer tissues, inversely correlated with ERLIN2. We further demonstrated the downregulation of miR-410 in breast cancer might be due to the hypermethylation of its promoter. Conclusions: Our study indicates that miR-410 suppresses cell growth, migration and invasion by directly downregulating ERLIN2 in ER positive breast cancer, acting as a tumor suppressor. Our study also suggests that miR-410 may serve as a potential therapeutic target for patients with ER positive breast cancer.

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Section: Discussionmentioning

confidence: 48%

MiR-410 Acts as a Tumor Suppressor in Estrogen Receptor-Positive Breast Cancer Cells by Directly Targeting ERLIN2 via the ERS Pathway

Guo

et al. 2018

Cell Physiol Biochem

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“…In our earlier research (Huang et al, 2013), laryngeal CSCs were harvested and accepted to radiation stress. We applied microRNA biochips to identify and screen differential expression miRNAs, and more than 2-fold up-regulation/down-regulation expression were considered as differential expressions.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study (Huang et al, 2013), we used microRNA biochips to compare and screen the differential expression microRNAs in laryngeal CSCs in response to radiation stress. Based on the predicted genes and pathways of miRNA target, the expression profile of hsa-miR-138-2-3p that was down-regulated significantly after radiation was thought to play an important role in regulation of radio-sensitivity in laryngeal squamous CSCs.…”

Section: Introductionmentioning

confidence: 99%

Radiosensitization effect of hsa-miR-138-2-3p on human laryngeal cancer stem cells

Zhu

Shi

Lei

et al. 2017

PeerJ

Self Cite

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BackgroundTreatments that target cancer stem cells play an important role in the controlling and eliminating of tumor initiation as well as in development, progression, and chemotherapy/radiotherapy resistance. In our previous study, we cultured and harvested human laryngeal cancer stem cells (CSCs) and applied microRNA biochips to screen differentially expressed miRNAs that were related to radiation tolerance in irradiated human laryngeal CSCs. According to the predicted genes and pathways of differential miRNAs target, down-regulated expression of hsa-miR-138-2-3p under radiation was thought to play a key role in enhancing the radio-sensitivity in human laryngeal squamous cancer stem cells.MethodTo investigate the radiational enhancement of hsa-miR-138-2-3p, we transfected hsa-miR-138-2-3p mimics that were synthesized based on the sequences of hsa-miR-138-2-3p in vitrointo human laryngeal CSCs (Hep-2, M2e, and TU212 cell lines) to make hsa-miR-138-2-3p overexpressed, and the tumorous specialities of CSCs, like cell proliferation, invasion, apoptosis, cell cycle arrest, and DNA damage were evaluated by CCK-8 assay, clone formation assay, invasion assay, flow cytometry, and comet assay. Furthermore, we explored the signal transduction pathways that regulated the cancer stem cell initiation, development, invasion, apoptosis and cell cycle arrest, which were controlled by hsa-miR-138-2-3p.ResultOverexpressed hsa-miR-138-2-3p played a key role in many anti-cancer biological processes in human laryngeal CSCs: (1) it decreased laryngeal CSCs proliferation and invasion in response to radiotherapy; (2) it increased the proportion of early and late apoptosis in laryngeal CSCs after radiation, raised G1 phase arrest in laryngeal CSCs after radiation, and decreased the proportion of S stage cells of cell cycle that were related to radio-resistance in laryngeal CSCs; (3) it down-regulated the expression of β-catenin in Wnt signal pathway that was related to the tolerance of laryngeal CSCs to radiotherapy; (4) it down-regulated the expression of YAP1 in Hippo signal pathway that regulated cell proliferation, invasion and apoptosis; (5) it up-regulated the expression of p38 and JNK1 in MAPK signal pathway that was concerned to radio-sensitivity.ConclusionIn the present study, it was found that hsa-miR-138-2-3p regulated the Wnt/β-catenin pathways, the Hippo/YAP1 pathways, and the MAPK/p38/JNK1 pathways that were involved in cell proliferation, invasion, apoptosis, cell cycle arrest, radio-resistance and radio-sensitivity in laryngeal CSCs. These results will be useful for a better understanding of the cell biology of hsa-miR-138-2-3p in laryngeal CSCs, and for serving hsa-miR-138-2-3p as a promising biomarker and as a target for diagnosis and for novel anti-cancer therapies for laryngeal cancers.

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“…Hiroshi et al, 2013 Generation Sequencing and microarray platforms to establish a tumour specific gene expression, miRNA and methylome profiles in OSCC (Poage et al, 2012;Huang et al, 2013). Comparing the gene expression profile of OSCC stem cell with the tumour cells as controls may provide a differential expression pattern which may be useful in establishing the signature expression profile for OSCC stem cells.…”

Section: Sen Et Al 2011mentioning

confidence: 99%

Cancer Stem Cells and Stemness Markers in Oral Squamous Cell Carcinomas

Patel¹,

Shah²,

Shah³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Screening for MiRNAs Related to Laryngeal Squamous Carcinoma Stem Cell Radiation

Cited by 18 publications

References 24 publications

MiR-410 Acts as a Tumor Suppressor in Estrogen Receptor-Positive Breast Cancer Cells by Directly Targeting ERLIN2 via the ERS Pathway

MiR-410 Acts as a Tumor Suppressor in Estrogen Receptor-Positive Breast Cancer Cells by Directly Targeting ERLIN2 via the ERS Pathway

Radiosensitization effect of hsa-miR-138-2-3p on human laryngeal cancer stem cells

Cancer Stem Cells and Stemness Markers in Oral Squamous Cell Carcinomas

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