Screening for lactobacilli with probiotic properties in the infant gut microbiota

Kirtzalidou, Ekaterini; Pramateftaki, Paraskevi; Kotsou, Maria; Kyriacou, Adamantini

doi:10.1016/j.anaerobe.2011.05.007

Cited by 67 publications

(61 citation statements)

References 21 publications

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“…In the present study, among fifty two strains only six showed interesting viability at pH1.5 after 2 h of exposure. Previous reports indicated that pH (1.5-2) is the lethal acidity value for LAB isolated from human GI tract (Lankaputhra and Shah, 1995;Khalil et al, 2007;Xiaodong et al, 2009;Kirtzalidou et al, 2011). In the stomach, in addition to low pH, the probiotics are, also, subjected to the stress of proteolytic enzyme, pepsin (Holzapfel et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Lactobacillus strains isolated from Algerian children faeces for their probiotic properties

Bahri¹,

Lejeune²,

Dubois-Dauphin³

et al. 2014

Afr. J. Microbiol. Res.

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Lactic acid bacteria termed probiotics have preventive as well as curative effects on several types of diarrhoea of different aetiologies. The main objective of this study was to screen lactobacilli strains having probiotic traits, isolated from Algerian healthy children faeces on the purpose of using them further in children diarrheal illnesses. One hundred and twenty (120) lactic acid bacteria isolates were selected from faecal samples of healthy Algerian children aged between five and ten years. Gram positive rods and catalase negative bacteria (52 isolates) were screened, in vitro, for their probiotic potential properties including ability to survive in simulated gastro-intestinal conditions, adherence to Caco-2 cells and their antimicrobial activity. The results show that only five strains resisted in simulated gastric juice at pH 1.5 and pepsin. Four of them were resistant to simulated intestinal conditions at pH 8 and pancreatin and have a good adherence. In the end, three of them were retained as they display interesting probiotic profiles characterized by a strong antimicrobial effect against some intestinal pathogenic bacteria. They were identified by 16S rDNA sequencing as Lactobacillus plantarum F12, Lactobacillus brevis G6 and Lactobacillus paracasei B13.

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Section: Discussionmentioning

confidence: 99%

Characterization of Lactobacillus strains isolated from Algerian children faeces for their probiotic properties

Bahri¹,

Lejeune²,

Dubois-Dauphin³

et al. 2014

Afr. J. Microbiol. Res.

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“…Adherence and colonization of Lactobacillus strains in the gastrointestinal tract is the prerequisite for the strains to exhibit beneficial effect on humans (von Ossowski et al, 2010). The assessment of autoaggregation, coaggregation, hydrophobicity, and adherence to intestinal epithelial cell lines (Caco-2 and HT-29 cells) of Lactobacillus strains has been used as an in vitro method to screen strains having probiotic potential (Kos et al, 2003;Vlková et al, 2008;Tamang et al, 2009;Kirtzalidou et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Aggregation and adhesion properties of 22 Lactobacillus strains

Tuo

et al. 2013

Journal of Dairy Science

152

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In this paper, the autoaggregating, coaggregating, hydrophobicity, and adhering abilities of 22 Lactobacillus strains belonging to different species were assessed. No correlation existed between autoaggregation and adhesion of the strains belonging to different species, whereas a positive correlation existed between autoaggregation and adhesion of the strains belonging to the same species. After treating with guanidine HCl, the autoaggregating and adhering abilities of some Lactobacillus strains decreased, indicating that surface-bound proteins and other macromolecules played a role in the adhering and autoaggregating abilities. The strains Lactobacillus plantarum 20 and 66 had higher adhesion and coaggregation abilities and should be further studied for their probable probiotic properties. Aggregating, coaggregating, and adhering abilities of Lactobacillus strains could be used as the preliminary criteria for selecting strains having probiotic potential.

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“…Not all strains of lactobacilli and bifidobacteria can be used as components of probiotics and probiotic foods, but only those that are of human origin, non-pathogenic, resistant to gastric acid, bile and to the antibiotics, administered in medical practice; they should also have the potential to adhere to the gut epithelial tissue and produce antimicrobial substances; they should allow the conduction of technological processes, in which high concentrations of viable cells are obtained as well as to allow industrial cultivation, encapsulation and freezedrying and they should remain active during storage [11,12]. This requires the mandatory selection of strains of the genera Lactobacillus and Bifidobacterium with probiotic properties.…”

Section: Introductionmentioning

confidence: 99%