Screening for inhibitors of 2-oxoglutarate-dependent dioxygenases: Flavanone 3β-hydroxylase and flavonol synthase

Halbwirth, Heidi; Fischer, Thilo C.; Schlangen, Karin; Rademacher, W.; Schleifer, Klaus‐Jürgen; Forkmann, G.; Stich, Karl

doi:10.1016/j.plantsci.2006.03.014

Cited by 40 publications

(37 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…With the regular expressions of upstream PlCHS and downstream PlFLS, as well as lower expressions of PlF3H, PlF3'H and downstream PlDFR, PlANS, PlF3GT, fewer amount of anthocyanins and anthoxanthins was produced after Pro-Ca treatment. Furthermore, F3H, FLS and ANS were the 2-ODDs in the flavonoids biosynthesis pathway (Halbwirth et al, 2006). Lower expression of PlF3H and PlANS might indicate that F3H and ANS were the possible enzymes inhibited by ProCa treatment, while F3'H, DFR and UGT might also be suppressed (Fig.…”

Section: Discussionmentioning

confidence: 94%

“…Pro-Ca, a 2-ODD inhibitor, performed by blocking several 2-ODDs involved in the flavonoids biosynthesis pathway (Rademacher, 2000). Earlier, Halbwirth et al (2006) proposed that F3H and FLS, the two 2-ODDs genes, were inhibited in young grapevine leaves, flowers, and berries after Pro-Ca treatment. The inhibition of F3H had been proved in the skin of apples, while the expression levels of PAL, CHS, F3H, DFR, ANS and FGT were also reduced in varying degrees after treatment (Bizjak et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…It could lead to the inhibition of the activity of 2-ODDs for the reason that this competitive Pro-Ca to the 2-ODDs on the combination of substrates. Several 2-ODDs are involved in the late steps of gibberellins biosynthesis (Rademacher, 2000) and in the flavonoid biosynthesis (Halbwirth et al, 2006). Thus Pro-Ca could bring about inhibition in these biosyntheses and contribute to multiple positive biochemical effects.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prohexadione-calcium Modified Color Characteristics and Flavonoid Biosynthetic Genes Expression in Herbaceous Peony (Paeonia lactiflora) Flowers

Tang¹,

Zhao²,

Hao³

et al. 2017

IJAB

View full text Add to dashboard Cite

Prohexadione-calcium (Pro-Ca), originally developed as a plant growth retardant, has recently been discovered it could modify floral color in several ornamental plants like petunia and rose. However, the underlying mechanism of Pro-Ca on the regulation of floral color is still elusive. In this study, a purple-red herbaceous peony (Paeonia lactiflora Pall.) cultivar with control and Pro-Ca treatment was used as the material. Their color indices were measured, flavonoid compositions and contents as well as its biosynthetic genes expression were analyzed. After Pro-Ca treatment, a remarkable rise in the color parameter lightness (L * ) value along with an obvious fall in the color parameter red/green (a * ) value indicated that the loss of red color was occurred. In addition, total anthocyanin, anthoxanthin and flavonoid contents were all decreased significantly after Pro-Ca treatment, which was in accordance with the color indices. Furthermore, the expression patterns of most relative genes in flavonoid biosynthetic pathway expressed an overall decline after Pro-Ca treatment. Among the 2-oxoglutaric aciddependent dioxygenase genes involved in the pathway, flavanone 3-hydroxylase gene (F3H) and anthocyanidin synthase gene (ANS) had the highest levels of expression, suggesting that the loss of red color might be the reason of the synergistic inhibitory effect of these enzymes especially F3H and ANS in response to Pro-Ca. These results would contribute to a theoretical foundation for the regulation of floral color in field P. lactiflora by Pro-Ca treatment.

show abstract

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prohexadione-calcium Modified Color Characteristics and Flavonoid Biosynthetic Genes Expression in Herbaceous Peony (Paeonia lactiflora) Flowers

Tang¹,

Zhao²,

Hao³

et al. 2017

IJAB

View full text Add to dashboard Cite

show abstract

“…The GtFLS cDNA (DDBJ accession number AB587658) was 1,267 bp in length and contained an ORF encoding 333 deduced amino acid sequences. The deduced amino acid sequences of GtFLS exhibited 78.9, 70.9, and 69.9% identities with those of petunia (Z22543; Holton et al 1993), apple (AY965343; Halbwirth et al 2006) and Citrus unshiu (AB011796; Moriguchi et al 2002), respectively. FLS belongs to the 2-oxoglutarate-dependent dioxygenase family, along with anthocyanidin synthase (ANS) and F3H.…”

Section: Isolation Of a Fls Gene From Gentian Anthersmentioning

confidence: 98%

A flavonol synthase gene GtFLS defines anther-specific flavonol accumulation in gentian

Kimura

Nakatsuka

Yamada

et al. 2010

Plant Biotechnology

View full text Add to dashboard Cite

Flavonoids are important for male gametophyte development. Here we report on the flavonoid components and their biosynthetic regulation in gentian anthers. Among flavonoids, flavonols, including kaempferol, quercetin and isorhamnetin derivatives, accumulated abundantly in gentian anthers. However, flavones and anthocyanins, which are the main flavonoids accumulating in petals, were not detected. Northern blot analysis of nine flavonoid biosynthetic genes showed that the 'early' flavonoid biosynthetic genes were expressed in both anthers and petals, and that flavonol synthase (FLS) transcripts were restricted to anthers. In contrast, flavone synthase II (FNSII) and 'late' flavonoid biosynthetic genes were expressed specifically in gentian petals. To confirm anther-specific expression of FLS, the 5Ј-upstream region of FLS (GtFLSpro) was cloned by inverse PCR and fused to the uidA (GUS) reporter gene. Tobacco, Arabidopsis and gentian plants, transformed with the GtFLSproGUS construct, exhibited anther-specific GUS expression. Expression was observed in the tapetum and in pollen at late stages of anther development in transgenic plants. These results revealed that flavonol accumulation in gentian anthers was regulated by the spatial expression of GtFLS. Our results also suggest that antherspecific regulation of FLS is conserved among higher plants and the GtFLS promoter is useful for induction of specific gene expression in anthers.Key words: Anther, flavonol synthase, gentian, promoter, transgenic plants. Plant Biotechnology28, 211-221 (2010) DOI: 10.5511/plantbiotechnology.10.1109c Original PaperAbbreviations: ANS, anthocyanidin synthase; CHI, chalcone isomerase; CHS, chalcone synthase; DFR, dihydroflavonol 4-reductase; F3H, flavanone 3-hydroxylase; F3ЈH, flavonoid 3Ј-hydroxylase; F3Ј5ЈH, flavonoid 3Ј,5Ј-hydroxylase; FLS, flavonol synthase; FNSII, flavone synthase II; HPLC, high performance liquid chromatography; RACE, rapid amplification of cDNA ends The nucleotide sequences reported in this paper have been submitted to DDBJ under accession numbers AB587658 and AB587659. † The authors contributed equally to this work. This article can be found at

show abstract

“…Apigeninidin, luteolinidin and their derivatives, which are also 3-deoxy flavonoids, act as phytoalexins in Sorghum bicolor (Lo et al, 1999). For more information see Römmelt et al (2003), Halbwirth et al (2003), Rademacher (2004b) and Halbwirth et al (2006). Prohexadione-calcium and trinexapac-ethyl reduce ethylene formation in sunflower cell suspensions and in leaf disks of wheat (Grossmann, 1992).…”

Section: Structural Mimics Of 2-oxoglutaric Acidmentioning

confidence: 99%

Chemical Regulators of Gibberellin Status and Their Application in Plant Production

Rademacher¹

2017

Annual Plant Reviews Online

Self Cite

View full text Add to dashboard Cite

Gibberellins and inhibitors of their biosynthesis are widely used in modern agriculture, horticulture and viticulture. Their global market is in the range of US$ 500 million. The gibberellins GA 3 , GA 4 and GA 7 are primarily used to increase fruit yield and/or quality in fruit trees and table and wine grapes. Distinct steps of the gibberellin biosynthetic pathway can be inhibited by growth retardants: chlormequat and mepiquat chloride, ancymidol, flurprimidol, paclobutrazol, uniconazole, and the fungicides tebuconazole and metconazole and daminozide, trinexapac-ethyl and prohexadione-calcium are currently used in crop production. Growth retardants reduce shoot elongation, thereby lowering the risk of lodging in cereals, rice and oilseed rape, and making ornamentals more compact. A better canopy structure with improved formation of reproductive structures is induced in cotton and peanuts. In fruit and nut trees, less pruning is required and increases in crop yield and quality are obtained.

show abstract

Screening for inhibitors of 2-oxoglutarate-dependent dioxygenases: Flavanone 3β-hydroxylase and flavonol synthase

Cited by 40 publications

References 22 publications

Prohexadione-calcium Modified Color Characteristics and Flavonoid Biosynthetic Genes Expression in Herbaceous Peony (Paeonia lactiflora) Flowers

Prohexadione-calcium Modified Color Characteristics and Flavonoid Biosynthetic Genes Expression in Herbaceous Peony (Paeonia lactiflora) Flowers

A flavonol synthase gene GtFLS defines anther-specific flavonol accumulation in gentian

Chemical Regulators of Gibberellin Status and Their Application in Plant Production

Contact Info

Product

Resources

About