Screening for enterocins and detection of hemolysin and vancomycin resistance in enterococci of different origins

Vuyst, Luc De; Moreno, M.R. Foulquié; Revets, Hilde

doi:10.1016/s0168-1605(02)00425-7

Cited by 245 publications

(212 citation statements)

References 79 publications

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“…Enterocin B gene was always found to be together with enterocin A gene. Similar to our results the enterocin B structural gene was always associated with the presence of enterocin B gene in previous studies due to the fact that no transport genes have been found for enterocin B producers [10,23].…”

Section: Resultssupporting

confidence: 92%

“…The similar results were reported in previous studies that most enterocin producing enterococci displayed antilisterial activity, while a fewer portion of them also showed antibacterial spectrum activity against Staphylococcus spp., Bacillus spp. and Clostridium spp [3,10,[16][17][18]. No correlation was found between the origin of the strains and the inhibitory spectrum.…”

Section: Resultsmentioning

confidence: 93%

“…It was reported that, in a collection of 61 enterococci isolated from environmental sources like surface and waste waters, sheep manure, enterocin structural gene positive strains were found to be 57.4% [22]. In another research, 64 of 122 (52.5%), inhibitory Enterococcus strains isolated from food and feed, animal, clinical and non-clinical samples, were found to harbour at least one enterocin structural gene [10]. None of the bacteriocin genes tested in this study were demonstrated in two strains of E. faecium (HBE-19, HBE-36) and E. hirae HBE-10, which showed antibacterial activity against S. aureus.…”

Section: Resultsmentioning

confidence: 99%

“…Although a screening for production of bacteriocins by E. faecalis and E. faecium was the subject of several previous studies [10][11][12][13][14], only little information is available about this feature in E. avium, E. hirae, E. mundtii, E. casseliflavus and E. durans isolated from different environments. The aim of this study was to screen the production of antimicrobial activities as well as the presence of bacteriocin structural genes in enterococcal isolates include diverse species isolated from different sources in Turkey.…”

Section: Introductionmentioning

confidence: 99%

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Phenotypic and Genotypic Characterization of Bacteriocins in Enterococcal Isolates of Different Sources

et al. 2011

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A collection of 57 enterococcal isolates from different origin (including river, treatment plant, spring and garbage water, soil, animal, and vegetables from Aydın) was screened for the production of bacteriocins. Enterococci were identified at species levels as Enterococcus faecium (34), E. hirae (6), E. casseliflavus (4), E. durans (4), E. faecalis (4), E. mundtii (3) and E. avium (2). Of the 57 isolates 40 of them inhibited the growth of at least one indicator bacterium. Based on our PCR results 54 strains possesed enterocin genes. The genes of entA and entB were the most frequently detected structural genes among the PCR positive strains (54 and 53 strains, respectively) and the entB gene was always associated with entA gene. The highest combination of enterocin genes (24 of 54 strains) detected was entA, entB, entP and entL50A/B. The enterocins AS-48 and CylL LS genes were not found. Three enterococcal isolates, 2 E. faecium and 1 E. hirae were not harbour any of tested enterocin genes. No correlation between the presence of enterocin structural genes and the origin of the strain was detected, also no relationship seemed to exist between the tested enterocin genes and the activity spectra of isolates. Genes encoding bacteriocins are widely disseminated among enterocci from different origin and more studies should be done for evaluate industrial potential of bacteriocins.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phenotypic and Genotypic Characterization of Bacteriocins in Enterococcal Isolates of Different Sources

et al. 2011

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“…Almost 70% of the isolates exhibited antimicrobial activity against one or more indicator bacteria. This corresponds to a higher proportion than often described in other investigations (9,12,21). Among lactic acid bacteria isolated from raw milk, 82 of 298 strains (27.5%) displayed bacteriocin activity (21), while 8.7% of S. aureus strains isolated from cattle produced antimicrobial substances (17).…”

Section: Discussionmentioning

confidence: 64%

Screening for antimicrobial activity among bacteria isolated from the Amazon Basin

Motta¹,

Cladera-Olivera²,

Brandelli³

2004

Braz. J. Microbiol.

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Bacteria producing antimicrobial activity were identified among 86 isolates from aquatic environments of Brazilian Amazon Basin. Antimicrobial activity against at least one indicator strain was detected for 59 isolates (68.6%). Inhibitory activity was mostly against Gram-positive bacteria, such as Listeria monocytogenes and Bacillus cereus. The antimicrobial substances produced by 19 strains that showed higher inhibitory activity were partially characterized. These antimicrobial substances showed thermal resistance for up to 100°C and partial resistance to proteolytic treatment. Some antimicrobials were partially resistant to pronase E at 2 mg ml -1 or to treatment with trichloroacetic acid. Detection of antimicrobial activity on polyacrylamide gels revealed that the molecular weight of the compounds was lower than 14 kDa. Several strains showed antibacterial activity, which in some cases appear to be related to antimicrobial peptides. The potential of these microorganisms to produce useful antimicrobial compounds is great and must be better explored.

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LAB strains with bacteriocin synthesis genes and their applications

Sacchini

Migliorati

Giannatale

et al. 2017

Microbiology in Dairy Processing

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Screening for enterocins and detection of hemolysin and vancomycin resistance in enterococci of different origins

Cited by 245 publications

References 79 publications

Phenotypic and Genotypic Characterization of Bacteriocins in Enterococcal Isolates of Different Sources

Phenotypic and Genotypic Characterization of Bacteriocins in Enterococcal Isolates of Different Sources

Screening for antimicrobial activity among bacteria isolated from the Amazon Basin

LAB strains with bacteriocin synthesis genes and their applications

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