Screening for drought tolerance in cotton (Gossypium hirsutum L.) using in vitro technique

Jaafar, Khadijat Suleiman; Mohammed, Maimuna Abdulmalik; Mohammed, Sagir Mohammed

doi:10.5897/joda2021.0067

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…G56 (0.427 g), G44 (0.424 g), the commercial variety G86 (0.411 g), G51 (0.397 g), and G47 (0.148 g), respectively (Supplementary Table S1). Our findings are similar to Iqbal et al [71], Mvula et al [63], and Jaafar et al [72]. Similarly, Shah et al [73] obtained a root weight ranging from 0.049 to 0.155 g under water stress in a trial conducted under normal and water-stressed conditions for 52 days.…”

Section: Discussionsupporting

confidence: 91%

Assessing Drought Tolerance in a Large Number of Upland Cotton Plants (Gossypium hirsutum L.) under Different Irrigation Regimes at the Seedling Stage

Çelik

2023

Life

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The cotton plant is important since it provides raw materials for various industry branches. Even though cotton is generally drought-tolerant, it is affected negatively by long-term drought stress. The trial was conducted according to the applied experimental design as a completely randomized design (CRD) with three replications to determine a panel of 93 cotton genotypes’ genotypic responses against drought under controlled conditions in 2022. All genotypes were watered with 80 mL−1 of water (100% irrigation, field capacity) until three true leaves appeared, and then water stress was applied at a limited irrigation of 75% (60 mL−1), 50% (40 mL−1), and 25% (20 mL−1) of the field capacity. After the trial terminated at 52 days, the cv. G56, G44, G5, and G86 in RL; G1, G56, G44, G86, G51, and G88 in RFW; advanced line G5, followed by the cv. G56, advanced line G44, G75, and the cv. G90 in RDW; G44, followed by G86, the cv. G56, and elite lines G13 and G5 in NLRs were observed as drought-tolerant genotypes, respectively, while G35, G15, G26, G67, and G56 in SL; G15, G52, G60, G31, and G68 in SFW; G35, G52, G57, G41, and G60 in SDW show the highest drought tolerance means, respectively. In conclusion, the commercial varieties with high means in roots, namely G86, G56, G88, and G90, and the genotypes G67, G20, G60, and G57 showing tolerance in shoots, are suggested to be potential parent plants for developing cotton varieties resistant to drought. Using the cultivars found tolerant in the current study as parents in a drought-tolerant variety development marker-assisted selection (MAS) plant breeding program will increase the chance of success in reaching the target after genetic diversity analyses are performed. On the other hand, it is highly recommended to continue the plant breeding program with the G44, G30, G19, G1, G5, G75, G35, G15, G52, G29, and G76 genotypes, which show high tolerance in both root and shoot systems.

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Section: Discussionsupporting

confidence: 91%

Assessing Drought Tolerance in a Large Number of Upland Cotton Plants (Gossypium hirsutum L.) under Different Irrigation Regimes at the Seedling Stage

Çelik

2023

Life

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“…Recent advances in genetic techniques, genomics tools, and breeding methodologies have opened promising avenues for identifying candidate genes and metabolic pathways underlying crop drought tolerance [ 65 ]. Marker-assisted breeding and the use of “omics” technologies have been recognized as effective for breeding drought tolerance, facilitating the identification of drought-related quantitative trait loci (QTLs) and enabling more efficient drought screening techniques [ 57 , 66 ]. In conclusion, while traditional breeding methods have historically played a pivotal role in crop improvement, their limitations in addressing complex traits like drought tolerance have led to the exploration and integration of modern biotechnological tools and genomics approaches.…”

Section: Traditional Breeding Vs Biotechnological Approachesmentioning

confidence: 99%

Advanced Biotechnological Interventions in Mitigating Drought Stress in Plants

Şimşek,

Isak,

Dönmez

et al. 2024

Plants

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This comprehensive article critically analyzes the advanced biotechnological strategies to mitigate plant drought stress. It encompasses an in-depth exploration of the latest developments in plant genomics, proteomics, and metabolomics, shedding light on the complex molecular mechanisms that plants employ to combat drought stress. The study also emphasizes the significant advancements in genetic engineering techniques, particularly CRISPR-Cas9 genome editing, which have revolutionized the creation of drought-resistant crop varieties. Furthermore, the article explores microbial biotechnology’s pivotal role, such as plant growth-promoting rhizobacteria (PGPR) and mycorrhizae, in enhancing plant resilience against drought conditions. The integration of these cutting-edge biotechnological interventions with traditional breeding methods is presented as a holistic approach for fortifying crops against drought stress. This integration addresses immediate agricultural needs and contributes significantly to sustainable agriculture, ensuring food security in the face of escalating climate change challenges.

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“…Compared to the control, the significantly lower fresh weight of Stevia rebaudiana leaves was also measured by (Hajihashemi and Sofo, 2018) in the treatment with PEG 15% (w/v). Also, (Jaafar et al, 2021) performed a screening of 12 lines of cotton and observed that shoot and root weight decreased with an increase in PEG concentration from 0 to 15%. (Zhong et al, 2018), also found that fresh shoot weight decreased with increasing concentration of PEG 8000 in kiwifruit (Actinidia sp.)…”

Section: Morphological Traitsmentioning

confidence: 99%

In vitro selection of drought-tolerant white poplar clones based on antioxidant activities and osmoprotectant content

Vuksanović,

Kovačević,

Kebert

et al. 2023

Front. Plant Sci.

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IntroductionIn light of upcoming climate change, there is an urgent requirement for tree improvement regarding adaptability to drought-caused stress and the development of quick and reliable screening methodologies for genotypes’ drought tolerance. White poplar is, despite its high adaptability, considered to be an endangered tree species in Serbia, which gives it special importance in the preservation and improvement of biodiversity of riparian ecosystems. The main goal of this research was to evaluate the tolerance of five white poplar clones to the presence of polyethylene glycol (PEG 6000 molecular weight 6000) (different concentrations (e.g. 0 g/L, 1 g/L, 10 g/L, 20 g/L, and 50 g/L) in Aspen Culture Medium (ACM).MethodsThe tolerance of the clones was evaluated by using morphological parameters (shoot fresh and dry weight, root fresh and dry weight), photosynthetic pigments (contents of chlorophyll a, chlorophyll b, carotenoids, and chlorophyll a+b), and biochemical parameters (total phenolic content, total flavonoid content, ferric reducing antioxidant power, antioxidant activities (DPPH activity and ABTS assay), free proline content and glycine betaine content.Results and DiscussionThe values of morphological and photosynthetic pigments declined with an increase in the concentration of PEG 6000. At a concentration of 50 g/L, the content of shoot fresh mass decreased by 41%, the content of Chl a by 68%, Chl b by 65%, and Car by 76% compared to the control. Also, at the same medium, there was an increase in the content of total phenols, accumulation of proline, the content of glycine betaine as well as in antioxidant activity. Based on the obtained results, it can be assumed that more drought-tolerant clones are characterized by high values for biomass, high content of photosynthetic pigments, and high content of proline and glycine betaine in conditions similar to drought in vitro. Clone L-80 showed better results in most of the tested parameters, especially compared to the reference clone Villafranca.

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Screening for drought tolerance in cotton (Gossypium hirsutum L.) using in vitro technique

Cited by 6 publications

References 12 publications

Assessing Drought Tolerance in a Large Number of Upland Cotton Plants (Gossypium hirsutum L.) under Different Irrigation Regimes at the Seedling Stage

Assessing Drought Tolerance in a Large Number of Upland Cotton Plants (Gossypium hirsutum L.) under Different Irrigation Regimes at the Seedling Stage

Advanced Biotechnological Interventions in Mitigating Drought Stress in Plants

In vitro selection of drought-tolerant white poplar clones based on antioxidant activities and osmoprotectant content

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