Screening for cysteine-stabilized scaffolds for developing proteolytic-resistant AMPs

Maximiano, Mariana Rocha; Rezende, Samilla B.; Rios, Thuanny Borba; Leite, Michel Lopes; Boas, Liana Costa Pereira Vilas; Cunha, Nicolau Brito da; Pires, Állan da Silva; Cardoso, Marlon Henrique; Franco, Octávio Luiz

doi:10.1016/bs.mie.2021.11.001

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…The effect of process on the total fungal count was occurred on the growth of fungal due to the temperature, moisture level and oxygen content. It also delays the stain, growth and development (Maximiano et al ., 2022). In our research, it confirmed the prepared noodles from the different BSG were safe for human consumption on the basis of fungal growth detection on the prepared sample.…”

Section: Resultsmentioning

confidence: 99%

Volarisation of Brewer's spent grain for noodles preparation and its potential assessment against obesity

Anisha

Kaushik

Kumar

et al. 2023

Int J of Food Sci Tech

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Brewer's spent grain is the most prevalent low-cost by-product of brewing industry, contributing to about 85% of all by-products produced. It is of interest for food industry applications because of its high nutritional value (fibres, protein and bioactive substances). The objective of this study was to determine the effects of different brewer's spent grain composition usage on various quality criteria of noodles as well as to investigate the possible usage opportunities and assessments of them in the production of noodles against obesity. For this purpose, noodle production was carried out using five different compositions of brewer's spent grain barley, barley and wheat, barley and rice, barley and maize, barley and finger millets, all at different concentrations (5%, 10%, 15% and 20%). Various quality criteria of the noodles were determined. Noodles made with the addition of barley and maize spent grains were found to be high in physio-chemical and nutritional properties, and amylase and lipase inhibition assay. Concentration above 15% of spent grains affected the texture of the noodles. From the sensory evaluation, 10% concentration of barley and maize noodles was selected as it had good texture, colour and appearance. It was comparatively good in taste and aroma from other samples.

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Section: Resultsmentioning

confidence: 99%

Volarisation of Brewer's spent grain for noodles preparation and its potential assessment against obesity

Anisha

Kaushik

Kumar

et al. 2023

Int J of Food Sci Tech

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Exploring the resistome, virulome, and mobilome of multidrug-resistant Klebsiella pneumoniae isolates: deciphering the molecular basis of carbapenem resistance

Rahmat Ullah,

Irum,

Mahnoor

et al. 2024

BMC Genomics

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Background Klebsiella pneumoniae, a notorious pathogen for causing nosocomial infections has become a major cause of neonatal septicemia, leading to high morbidity and mortality worldwide. This opportunistic bacterium has become highly resistant to antibiotics due to the widespread acquisition of genes encoding a variety of enzymes such as extended-spectrum beta-lactamases (ESBLs) and carbapenemases. We collected Klebsiella pneumoniae isolates from a local tertiary care hospital from February 2019–February 2021. To gain molecular insight into the resistome, virulome, and genetic environment of significant genes of multidrug-resistant K. pneumoniae isolates, we performed the short-read whole-genome sequencing of 10 K. pneumoniae isolates recovered from adult patients, neonates, and hospital tap water samples. Results The draft genomes of the isolates varied in size, ranging from 5.48 to 5.96 Mbp suggesting the genome plasticity of this pathogen. Various genes conferring resistance to different classes of antibiotics e.g., aminoglycosides, quinolones, sulfonamides, tetracycline, and trimethoprim were identified in all sequenced isolates. The highest resistance was observed towards carbapenems, which has been putatively linked to the presence of both class B and class D carbapenemases, blaNDM, and blaOXA, respectively. Moreover, the biocide resistance gene qacEdelta1 was found in 6/10 of the sequenced strains. The sequenced isolates exhibited a broad range of sequence types and capsular types. The significant antibiotic resistance genes (ARGs) were bracketed by a variety of mobile genetic elements (MGEs). Various spontaneous mutations in genes other than the acquired antibiotic-resistance genes were observed, which play an indirect role in making these bugs resistant to antibiotics. Loss or deficiency of outer membrane porins, combined with ESBL production, played a significant role in carbapenem resistance in our sequenced isolates. Phylogenetic analysis revealed that the study isolates exhibited evolutionary relationships with strains from China, India, and the USA suggesting a shared evolutionary history and potential dissemination of similar genes amongst the isolates of different origins. Conclusions This study provides valuable insight into the presence of multiple mechanisms of carbapenem resistance in K. pneumoniae strains including the acquisition of multiple antibiotic-resistance genes through mobile genetic elements. Identification of rich mobilome yielded insightful information regarding the crucial role of insertion sequences, transposons, and integrons in shaping the genome of bacteria for the transmission of various resistance-associated genes. Multi-drug resistant isolates that had the fewest resistance genes exhibited a significant number of mutations. K. pneumoniae isolate from water source displayed comparable antibiotic resistance determinants to clinical isolates and the highest number of virulence-associated genes suggesting the possible interplay of ARGs amongst bacteria from different sources.

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Screening for cysteine-stabilized scaffolds for developing proteolytic-resistant AMPs

Cited by 2 publications

References 27 publications

Volarisation of Brewer's spent grain for noodles preparation and its potential assessment against obesity

Volarisation of Brewer's spent grain for noodles preparation and its potential assessment against obesity

Exploring the resistome, virulome, and mobilome of multidrug-resistant Klebsiella pneumoniae isolates: deciphering the molecular basis of carbapenem resistance

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