Screening for boron tolerance in wheat (T. aestivum) by solution culture in filter paper

Chantachume, Y.; Smith, D. A.; Hollamby, G. J.; Paull, J. G.; Rathjen, A. J.

doi:10.1007/bf00010131

Cited by 46 publications

(23 citation statements)

References 15 publications

(18 reference statements)

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“…Inhibition of root elongation is one of the earliest and distinct symptoms of plant B toxicity (Chantachume et al 1995;Reid et al 2004). In the present study, we found that exposure of cucumber seedlings to 5 mM B rapidly inhibited root elongation and led to root curvature (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, plants suVering from B toxicity also exhibit increases in contents of malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ), resulting in oxidative stress and membrane peroxidation (Cervilla et al 2007(Cervilla et al , 2009ArdÂc et al 2009). One of the main symptoms of B toxicity is rapid inhibition of root elongation (Nable 1988;Holloway and Alston 1992;Chantachume et al 1995;Reid et al 2004;Choi et al 2007). It has been shown that the root apex is the critical site for sensing and expressing B toxicity .…”

Section: Introductionmentioning

confidence: 99%

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Boron toxicity is alleviated by hydrogen sulfide in cucumber (Cucumis sativus L.) seedlings

Wang

Shi

et al. 2010

Planta

157

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Boron (B) is an essential micronutrient for plants, which when occurs in excess in the growth medium, becomes toxic to plants. Rapid inhibition of root elongation is one of the most distinct symptoms of B toxicity. Hydrogen sulfide (H(2)S) is emerging as a potential messenger molecule involved in modulation of physiological processes in plants. In the present study, we investigated the role of H(2)S in B toxicity in cucumber (Cucumis sativus) seedlings. Root elongation was significantly inhibited by exposure of cucumber seedlings to solutions containing 5 mM B. The inhibitory effect of B on root elongation was substantially alleviated by treatment with H(2)S donor sodium hydrosulfide (NaHS). There was an increase in the activity of pectin methylesterase (PME) and up-regulated expression of genes encoding PME (CsPME) and expansin (CsExp) on exposure to high B concentration. The increase in PME activity and up-regulation of expression of CsPME and CsExp induced by high B concentration were markedly reduced in the presence of H(2)S donor. There was a rapid increase in soluble B concentrations in roots on exposure to high concentration B solutions. Treatment with H(2)S donor led to a transient reduction in soluble B concentration in roots such that no differences in soluble B concentrations in roots in the absence and presence of NaHS were found after 8 h exposure to the high concentration B solutions. These findings suggest that increases in activities of PME and expansin may underlie the inhibition of root elongation by toxic B, and that H(2)S plays an ameliorative role in protection of plants from B toxicity by counteracting B-induced up-regulation of cell wall-associated proteins of PME and expansins.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Boron toxicity is alleviated by hydrogen sulfide in cucumber (Cucumis sativus L.) seedlings

Wang

Shi

et al. 2010

Planta

157

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“…These early studies not only identified important germplasm forming the basis of B toxicity tolerance research in cereals, but also helped in the development of more efficient screening methods. A range of phenotypic assays for screening for B toxicity tolerance under controlled conditions have since been developed, and these include measurements of leaf tissue concentrations of B, relative root lengths, shoot dry weights and leaf symptom expression (Campbell et al 1998, Chantachume et al 1995, Jefferies et al 1999, Schnurbusch et al 2008. Given that exclusion of B appears to be the most important mechanism for tolerance, phenotypic screening should ideally involve analysis of tissue (shoot or root) B concentration.…”

Section: Phenotypic Screening Methods To Identify B Toxicity Tolerancmentioning

confidence: 99%

Boron toxicity tolerance in wheat and barley: Australian perspectives

2010

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“…Screening techniques previously used to identify B tolerance in germplasm include field screening (Torun et al, 2003) and controlled environment screening using both soil-based and solutionbased methods (Campbell et al, 1998) (Chantachume et al, 1995). High field spatial variability in the distribution of soil B in southern Australia (Nuttall et al, 2003b) makes field screening risky and difficult to interpret.…”

Section: Introductionmentioning

confidence: 99%

Response of lentil (Lens culinaris) germplasm to high concentrations of soil boron

Hobson

Armstrong²,

Nicolas

et al. 2006

Euphytica

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For lentil production to expand further in Australia, adaptation to the less favourable soils of the low to medium rainfall zones is required. To improve adaptation to these regions, varieties are required with increased tolerance to soil constraints such as high concentrations of boron (B), salinity and sodicity. To evaluate the range of B tolerance in lentil germplasm, 310 lines were screened in soil with a high concentration of B and tolerance was assessed at the seedling stage. A wide range in response to high concentrations of soil B was observed in the germplasm tested. Current Australian varieties were generally very intolerant to high concentrations of soil B. High levels of B tolerance was identified in germplasm originating from Afghanistan and Ethiopia. A subsequent experiment comparing lentils with different levels of B tolerance found that tolerant accessions (ILL213A and ILL2024) produced greater above and below ground biomass than intolerant accessions. The tolerant accessions had no significant yield loss under a high B treatment (extractable B = 18.20 mg/kg) compared to the control treatment (extractable B = 1.55 mg/kg). The large improvement in B tolerance, at soil concentrations typical of those found in the target regions, suggests there is potential to improve the tolerance level of adapted varieties and expand lentil production areas to regions with higher concentrations of soil B.

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Screening for boron tolerance in wheat (T. aestivum) by solution culture in filter paper

Cited by 46 publications

References 15 publications

Boron toxicity is alleviated by hydrogen sulfide in cucumber (Cucumis sativus L.) seedlings

Boron toxicity is alleviated by hydrogen sulfide in cucumber (Cucumis sativus L.) seedlings

Boron toxicity tolerance in wheat and barley: Australian perspectives

Response of lentil (Lens culinaris) germplasm to high concentrations of soil boron

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