Screening for bioactive secondary metabolites in Sri Lankan medicinal plants by microfractionation and targeted isolation of antimicrobial flavonoids from Derris scandens

Mohotti, Supun; Rajendran, Sanjeevan; Muhammad, Taj; Adhikari, Achyut; Burman, Robert; Silva, E D de; Göransson, Ulf; Hettiarachchi, Chamari; Gunasekera, Sunithi

doi:10.1016/j.jep.2019.112158

Cited by 40 publications

(26 citation statements)

References 59 publications

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“…Another recent improvement to the standard microdilution method was an attempt to annul the risk of a medium inhibiting antimicrobial activity and improve extract solubility at the same time ( 64 , 65 ). In the first step, bacteria in the mid-logarithmic growth phase are washed twice by centrifugation, re-suspended in Tris buffer, and incubated with a polyphenol without a growth medium.…”

Section: Dilution Methodsmentioning

confidence: 99%

Challenges to antimicrobial susceptibility testing of plant-derived polyphenolic compounds

Bubonja‐Šonje

Knežević

Abram

2020

Archives of Industrial Hygiene and Toxicology

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As multidrug resistance gains momentum, the last two decades have seen an ever-growing interest in the antimicrobial properties of plant extracts and plant-derived compounds. Most of the focus is on polyphenols – a large and diverse group of phytochemicals with strong antibacterial activity. Testing methods provide reliable results as long as they follow standard procedures. However, methods and procedures used in antimicrobial susceptibility testing (AST) are often too diverse to allow comparison of results. The lack of uniformity and comparability is much owed to the absence of guidelines. The focus of this review is to give a critical overview of different methods used in the assessment of polyphenols antimicrobial efficacy and to highlight the importance of their standardisation.

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Section: Dilution Methodsmentioning

confidence: 99%

Challenges to antimicrobial susceptibility testing of plant-derived polyphenolic compounds

Bubonja‐Šonje

Knežević

Abram

2020

Archives of Industrial Hygiene and Toxicology

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“…Flavonoids have been found to be effective antifungal agents against a wide range of pathogenic organisms represented in Table 1 [86,[96][97][98][99][100][101][102][103][104]. Several in vivo and clinical investigations have reported that the flavonoids show various pharmacological functions; viz., anti-oxidant [68,69], antidiabetic [70], anti-obesity [71], anti-hyperlipidemic [72], anti-inflammatory [73], antiosteoporotic effect [74], antiallergic and antithrombotic [75], hepatoprotective [76], neuroprotective [77], renoprotective [78], chemopreventive and anticancer [79,80], anti-bacterial, antifungal, and anti-viral activities [81][82][83][84].…”

Section: Antifungal Activities Of Flavonoidsmentioning

confidence: 99%

“…Flavonoids have been found to be effective antifungal agents against a wide range of pathogenic organisms represented in Table 1 [86,[96][97][98][99][100][101][102][103][104]. The screening of antifungal flavonoids from plants has been assayed by using broth dilution, spore germination, and agar well or the disk diffusion.…”

Section: Antifungal Activities Of Flavonoidsmentioning

confidence: 99%

Anti-Fungal Efficacy and Mechanisms of Flavonoids

2020

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The prevalence of fungal infections is growing at an alarming pace and the pathogenesis is still not clearly understood. Recurrence of these fungal diseases is often due to their evolutionary avoidance of antifungal resistance. The development of suitable novel antimicrobial agents for fungal diseases continues to be a major problem in the current clinical field. Hence, it is urgently necessary to develop surrogate agents that are more effective than conventional available drugs. Among the remarkable innovations from earlier investigations on natural-drugs, flavonoids are a group of plant-derived substances capable of promoting many valuable effects on humans. The identification of flavonoids with possible antifungal effects at small concentrations or in synergistic combinations could help to overcome this problem. A combination of flavonoids with available drugs is an excellent approach to reduce the side effects and toxicity. This review focuses on various naturally occurring flavonoids and their antifungal activities, modes of action, and synergetic use in combination with conventional drugs.

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“…Thin-layer chromatography (TLC) micro-fractionation of the organic extracts of Ferula ferulioides [34], a traditional medicinal plant, served as a guiding tool to isolate two compounds (dalpanitin and vicenin-3), with remarkable antimicrobial activity against drug-resistant Staphylococcus aureus [35].…”

Section: Natural Extracts With Antimicrobial Activitymentioning

confidence: 99%

Nanotechnologies: An Innovative Tool to Release Natural Extracts with Antimicrobial Properties

et al. 2021

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Site-Specific release of active molecules with antimicrobial activity spurred the interest in the development of innovative polymeric nanocarriers. In the preparation of polymeric devices, nanotechnologies usually overcome the inconvenience frequently related to other synthetic strategies. High performing nanocarriers were synthesized using a wide range of starting polymer structures, with tailored features and great chemical versatility. Over the last decade, many antimicrobial substances originating from plants, herbs, and agro-food waste by-products were deeply investigated, significantly catching the interest of the scientific community. In this review, the most innovative strategies to synthesize nanodevices able to release antimicrobial natural extracts were discussed. In this regard, the properties and structure of the starting polymers, either synthetic or natural, as well as the antimicrobial activity of the biomolecules were deeply investigated, outlining the right combination able to inhibit pathogens in specific biological compartments.

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Screening for bioactive secondary metabolites in Sri Lankan medicinal plants by microfractionation and targeted isolation of antimicrobial flavonoids from Derris scandens

Cited by 40 publications

References 59 publications

Challenges to antimicrobial susceptibility testing of plant-derived polyphenolic compounds

Challenges to antimicrobial susceptibility testing of plant-derived polyphenolic compounds

Anti-Fungal Efficacy and Mechanisms of Flavonoids

Nanotechnologies: An Innovative Tool to Release Natural Extracts with Antimicrobial Properties

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