Screening for Antioxidant Activity in Edible Plant Products:  Comparison of Low-Density Lipoprotein Oxidation Assay, DPPH Radical Scavenging Assay, and Folin−Ciocalteu Assay

Katšube, Takao; Tabata, Hiromasa; Ohta, Yutaka; Yamasaki, Yukikazu; Anuurad, Erdembileg; Shiwaku, Kuninori; Yamane, Yoshihiro

doi:10.1021/jf035372g

Cited by 280 publications

(229 citation statements)

References 27 publications

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“…The TPC of the fresh apple was 6.1-fold lower than the average TPC of dried apples. Antioxidant capacity can be predicted based on the phenolic content (Katsube et al 2004). Results obtained in this study are in compliance with this statement -antioxidant capacity of dried apples determined by ABTS assay (Figure 3), was the highest in the sample 9, and the lowest in sample 4, while results obtained from FRAP assay (Figure 3), were the highest in sample 9 and the lowest in sample 7.…”

Section: Resultssupporting

confidence: 84%

The Effect of Ultrasound Pre-Treatment and Air-Drying on the Quality of Dried Apples

Opalić¹,

Domitran²,

Komes³

et al. 2009

Czech J. Food Sci.

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Abstract:In order to develop environmentally sound, energy inexpensive and well scalable drying techniques that maintain high quality of dried fruit, optimisation of integrated process (ultrasound and air-drying) in the production of dried apples was conducted. Selected quality parameters of fresh and dried apples (variety Goldparmâne) resulting from different duration of ultrasonic pre-treatment and air-drying were compared. Sugars were determined spectrophotometrically using an enzymatic method. Content of total phenols and flavonoids was determined spectrophotometrically with the Folin-Ciocalteau assay, while the antioxidant capacity was evaluated by using ABTS (2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and FRAP (Ferric Reducing/Antioxidant Power) assays. Sensory properties of dried apples were investigated according to the Quantitative Descriptive Analysis. In combination with the same air-drying conditions, prolonged ultrasound pre-treatment led to a decrease in total phenols and flavonoids, as well as in the antioxidant capacity of dried apples. Difference in drying time had no significant effect on the content of total phenols and flavonoids, as well as antioxidant capacity. The sample dried without the ultrasound pre-treatment was evaluated as the most sensory acceptable. The content of glucose and fructose correlated well with total phenols and flavonoids, as well as with antioxidant capacity of apple samples.

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Section: Resultssupporting

confidence: 84%

The Effect of Ultrasound Pre-Treatment and Air-Drying on the Quality of Dried Apples

Opalić¹,

Domitran²,

Komes³

et al. 2009

Czech J. Food Sci.

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“…This figure shows that the data correlate very well, with a slope close to one. Similar results have been reported for a large number of edible plant products 18 (R = 0.969), for tropical fruits 36 (R = 0.96; N = 14), for herbal teas 37 (R = 0.93, N = 10) and for commercially available teas in Argentina 33 ( R 2 = 0.914, N = 12). Nevertheless, in other systems the relationship is considerably weaker 38 .…”

Section: Resultssupporting

confidence: 85%

“…In the present work we discuss the validity of the proposed methodologies, with particular emphasis on the information that can be derived from kinetic evaluations of the rate of DPPH bleaching and the stoichiometry of the process. This is a conflicting subject and even there are not consensus regarding how to estimate the "final" DPPH concentration: 5 minutes 18 24 or reaching a plateau 25 or a steady state condition 26,27 .…”

Section: Introductionmentioning

confidence: 99%

Kinetic and Stoichiometric Evaluation of Free Radicals Scavengers Activities Based on Diphenyl-Picryl Hydrazyyl (Dpph) Consumption

Campos

Duran

López-Alarcón

et al. 2012

J. Chil. Chem. Soc.

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Kinetics and stoichiometric measurements based on the reactions of stable free radicals are among the most employed procedures in the evaluation of antioxidant activities of pure compounds and complex mixtures. In the present work it is shown that initial rates of diphenyl-picryl hydrazyl (DPPH) consumption elicited by cinnamic acid derivatives are closely related to their reactivity towards peroxyl radicals. This implies that kinetic data regarding DPPH bleaching can be useful on the evaluation of antioxidant reactivities. On the other hand, data obtained at a single (long) reaction time are unrelated to the reactivity of the antioxidants and mostly reflect the number of phenol groups present in the tested sample

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“…The 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of Senna italica fractions were determined according to the method described by Katsube et al (2004). The assay involves the measurement of the disappearance of the coloured free radical, DPPH, by spectrophotometric determination.…”

Section: Quantitative Dpph Radical Scavenging Activity Assaymentioning

confidence: 99%

Evaluation of the antioxidant, antibacterial, and antiproliferative activities of the acetone extract of the roots of Senna italica (fabaceae)

Masoko

Gololo

Mokgotho

et al. 2010

Afr. J. Trad. Compl. Alt. Med.

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Senna italica, a member of the Fabaceae family (subfamily Caesalpinaceae), is widely used traditionally to treat a number of disease conditions, such as sexually transmitted diseases and some forms of intestinal complications. The roots of Senna italica were collected from Zebediela subregion, Limpopo province (S.A), powdered and extracted with acetone by cold/shaking extraction method. The phytochemical composition of the extract was determined by thin layer chromatography (TLC). The chromatograms were visualised with vanillin-sulphuric acid and p-anisaldehyde reagents. The total phenolic content of the extract was determined by Folin-Ciocalteu method and expressed as TAE/g dry weight. The extract was assayed for the in vitro anticancer activity using Jurkat T cells, antioxidant activity using DPPH assay and antibacterial activity by bioautographic method and the microtitre plate method. The acetone extract of the roots of Senna italica inhibited the growth of Jurkat T cells in a dose-and time-dependent manner. The extract also had free radical scavenging activity as well as reasonable antibacterial activity against Pseudomonas aeruginosa, Enterococcus faecalis, Escherichia coli and Staphylococcus aureus with MICs ranging from 0,08 to 0.16 mg/ml in the same order as ampicillin the positive control. The biological activities observed in the acetone extract validated the ethnomedicinal use of Senna italica.

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Screening for Antioxidant Activity in Edible Plant Products: Comparison of Low-Density Lipoprotein Oxidation Assay, DPPH Radical Scavenging Assay, and Folin−Ciocalteu Assay

Cited by 280 publications

References 27 publications

The Effect of Ultrasound Pre-Treatment and Air-Drying on the Quality of Dried Apples

The Effect of Ultrasound Pre-Treatment and Air-Drying on the Quality of Dried Apples

Kinetic and Stoichiometric Evaluation of Free Radicals Scavengers Activities Based on Diphenyl-Picryl Hydrazyyl (Dpph) Consumption

Evaluation of the antioxidant, antibacterial, and antiproliferative activities of the acetone extract of the roots of Senna italica (fabaceae)

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