Screening, detection and antimicrobial susceptibility of multi-drug resistant pathogens from the clinical specimens

Kabeerdass, Nivedhitha; Krishnamoorthy, Sivakumar; Anbazhagan, Murugesan; Ramamurthy, Srinivasan; Senthilkumar, N.; Rajendran, Manikandan; Mathanmohun, Maghimaa

doi:10.1016/j.matpr.2021.05.018

Cited by 11 publications

(8 citation statements)

References 17 publications

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“…The crude of the resin obtained by ethanolic extraction shows high yield than compared with the aqueous extraction by maintaining different parameters like time (5,10,15,20), temperature (200W, 300W, 500W, and 700W), pH (6,7,8,9) concentration (100, 100, 100, 100). Therefore, the crude ethanolic resin obtained at 15minutes at the temperature of 700W provides a good yield and is shown in The GC-MS analysis explored eleven bioactive compounds in the ethanolic extract.…”

Section: Resultsmentioning

confidence: 99%

“…Encircling the Namakkal district approximately twenty samples were collected from the different ulcer, diabetic and sore patients [ 8 , 9 ]. Using sterile swabs swabbed the pus, wound, and other exudates then which are packed aseptically in a transport media containing polypropylene container, sealed and marked the history of the specimen and stored at -4 degree C brought to our laboratory for research work, received from various sophisticated labs and hospitals.…”

Section: Methodsmentioning

confidence: 99%

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Molecular docking and in vitro analysis of phytoextracts from B. serrata for antibacterial activities

Vakayil¹

2021

Bioinformation

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The bioactives of Boswellia serrata have a role in ulcer healing therapies. Eleven bioactive compounds were obtained by GC-MS among which Cholan-24-oic acid, 3,12-bis (acetyl oxy) has a high molecular weight of 490.6719 with a retention time of 26.729. Twenty wound samples were collected aseptically from the labs and hospitals in and around the Namakkal districts of Tamilnadu, India. The antibacterial potential of E.coli showed a maximum inhibition of 27 mm against Tetracycline at 30μg. The ethanolic extract of the B. serrata shows a susceptibility of 19mm towards E. coli at 60μg concentration in MIC. Molecular docking results show the binding energy of Cholan-24-oic acid, 3,12-bis(acetyloxy) -8.6 (kcal/mol) followed by Pyrene, hexadecahydro- -6.7 (kcal/mol), and 5(1H)- Azulenone, 2,4,6,7,8,8a-hexahydro-3,8-dimethyl-4-(1-methylethylidene)-, (8S-cis)- 6.4 (kcal/mol) for further consideration.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Molecular docking and in vitro analysis of phytoextracts from B. serrata for antibacterial activities

Vakayil¹

2021

Bioinformation

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“…These botanical oral silver nanotherapies stable their importance in the wound repairing process. This work acknowledges that the metal microparticles along with Alternanthera sessilis enhance and pave the way for scavenging free radicals because it is toxic-free, eco-friendly acts as a vehicle [22,23] to treat the bio lm on the ulcer. Figure 5 Spectrum of FT-IR analysis of synthesized AS-AgNPS The synthesized AS-AgNPs exposed the subsistence of carboxylic, alkene groups, and carbonyl groups.…”

Section: Discussionmentioning

confidence: 99%

Phyto Mediated Silver Nanoparticle Synthesis Coated Fabrics Towards Antibacterial Potential

Kabeerdass

Murugesan

Srinivasan

et al. 2021

Preprint

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Sore repairing since occurrence up to dry skin formation (scar) is a precious process gifted by nature. Immunology takes a predominant part in healing. A sore which is sustained just now, within minutes/hours is usually ignored till its uninterrupted scar formation. When is delayed that persists for many days, many weeks, more months, even for years is keenly noticed and is seeking secondary healing which is donated by medical men. Uninterrupted natural healing is usually processed by the immune system of the host when healing is delayed then comes interruption of the medical experts with the inclusive of medicines such as synthetic chemicals, phytotherapy, micro nano particles mingled medicines, botanical flora yield Phyto metal micro therapies which going for updated management like 3D bioprinting is in practice. An array of twenty exudates of nasal, pus, urine, stool, and blood are collected from the diverse age group of sex in various wards of SOTS (such as ICU, CCU, maternity, and casualty) from the Hospitals of Madurai. After isolating from other commensals its phenotypic character is studied through gram staining and biochemical tests. To get rid of their pathogenicity a cheap, cost-effective, eco-friendly potential medicinal ingredients possessed Amaranthaceae family yielded Alternanthera sessilis mediated fabrics coated silver nanoparticles were synthesized. In the case of MIC of AS-AgNPs against isolates zone of inhibition of the E. coli is 16mm > S. aureus is 15mm at 60µg. But in the case of MIC of fabrics against isolates the E. coli and K. oxytoca show susceptibility of 19 mm in mixed cotton. In a confirmation study of AS-AgNPs in FTIR, a sharp band is observed between 1500-500cm-1, in SEM with EDAX a graph was observed between 2.6–3.9 and black white crystals measured at 200nm in HR-TEM. Nanotechnology conjoined with herbal therapeutics provides a promising solution for wound management strategies.

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“…Gram-positive and Gram-negative pathogens such as Salmonella typhi , S. viridans , Staphylococcus aureus , S. epidermis , Candida albicans , Klebsiella pneumonia , Pseudomonas aeruginosa , Bacillus cereus , and Escherichia coli were aseptically inoculated in sealed containers. These pathogens were procured from the laboratory in Rasipuram, transported to the college laboratory, and stored in a refrigerator [ 16 , 17 ]. Later, they were subcultured in appropriate media, e.g., blood agar, nutrient agar, Eosin methylene agar, and MacConkey agar.…”

Section: Methodological Analysismentioning

confidence: 99%

Bacillus-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens

Kabeerdass¹,

Otaibi

Rajendran³

et al. 2021

Antibiotics

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In this article, the supernatant of the soil-borne pathogen Bacillus mn14 was used as the catalyst for the synthesis of AgNPs. The antibacterial and antifungal activity of Bs-AgNPs was evaluated, in which S. viridans and R. solani showed susceptibility at 70 µL and 100 µL concentrations. Enzyme properties of the isolates, according to minimal inhibitory action and a growth-enhancing hormone–indole acetic acid (IAA) study of the isolates, were expressed in TLC as a purple color with an Rf value of 0.7. UV/Vis spectroscopy revealed the presence of small-sized AgNPs, with a surface plasmon resonance (SPR) peak at 450 nm. The particle size analyzer identified the average diameter of the particles as 40.2 nm. The X-ray diffraction study confirmed the crystalline nature and face-centered cubic type of the silver nanoparticle. Scanning electron microscopy characterized the globular, small, round shape of the silver nanoparticle. AFM revealed the two-dimensional topology of the silver nanoparticle with a characteristic size ranging around 50 nm. Confocal microscopy showed the cell-wall disruption of S. viridans treated with Bs-AgNPs. High-content screening and compound microscopy revealed the destruction of mycelia of R. solani after exposure to Bs-AgNPs. Furthermore, the Bs-AgNPs cured sheath blight disease by reducing lesion length and enhancing root and shoot length in Oryza sativa seeds. This soil-borne pathogen Bacillus-mediated synthesis approach of AgNPs appears to be cost-efficient, ecofriendly, and farmer-friendly, representing an easy way of providing valuable nutritious edibles in the future.

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Screening, detection and antimicrobial susceptibility of multi-drug resistant pathogens from the clinical specimens

Cited by 11 publications

References 17 publications

Molecular docking and in vitro analysis of phytoextracts from B. serrata for antibacterial activities

Molecular docking and in vitro analysis of phytoextracts from B. serrata for antibacterial activities

Phyto Mediated Silver Nanoparticle Synthesis Coated Fabrics Towards Antibacterial Potential

Bacillus-Mediated Silver Nanoparticle Synthesis and Its Antagonistic Activity against Bacterial and Fungal Pathogens

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