Screening, cloning and expression of a novel alginate lyase gene from P. aeruginosa TAG 48 and its antibiofilm effects on P. aeruginosa biofilm

Tavafi, Hadis; Ali, Ahya Abdi; Ghadam, Parinaz; Gharavi, Sara

doi:10.1016/j.micpath.2018.08.018

Cited by 18 publications

(10 citation statements)

References 32 publications

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“…Bifunctional alginate lyase degrading both polyM and polyG could prevent growth and eradication of P. aeruginosa sp. TAG48 biofilm and exhibits synergy with tobramycin and cefixime but not with ciprofloxacin [93]. The results indicate that the use of purified novel alginate lyase with antibiotics could be a beneficial alternative for the treatment of P. aeruginosa infections.…”

Section: Antibiotic Applicationsmentioning

confidence: 92%

“…As a protective barrier, the thick biofilm is composed of several components [91]. Alginates are the major component of P. aeruginosa biofilm, which is responsible for surface adhesion and stabilization of biofilm to resist antibiotics [92,93]. Moreover, alginates can interrupt the phagocytosis of macrophages and neutrophils, limit the lymphocyte function, and induce inflammatory reactions to aggravate the lung infec-tion [91].…”

Section: Antibiotic Applicationsmentioning

confidence: 99%

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Structure Characteristics, Biochemical Properties, and Pharmaceutical Applications of Alginate Lyases

et al. 2021

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Alginate, the most abundant polysaccharides of brown algae, consists of various proportions of uronic acid epimers α-L-guluronic acid (G) and β-D-mannuronic acid (M). Alginate oligosaccharides (AOs), the degradation products of alginates, exhibit excellent bioactivities and a great potential for broad applications in pharmaceutical fields. Alginate lyases can degrade alginate to functional AOs with unsaturated bonds or monosaccharides, which can facilitate the biorefinery of brown algae. On account of the increasing applications of AOs and biorefinery of brown algae, there is a scientific need to explore the important aspects of alginate lyase, such as catalytic mechanism, structure, and property. This review covers fundamental aspects and recent developments in basic information, structural characteristics, the structure–substrate specificity or catalytic efficiency relationship, property, molecular modification, and applications. To meet the needs of biorefinery systems of a broad array of biochemical products, alginate lyases with special properties, such as salt-activated, wide pH adaptation range, and cold adaptation are outlined. Withal, various challenges in alginate lyase research are traced out, and future directions, specifically on the molecular biology part of alginate lyases, are delineated to further widen the horizon of these exceptional alginate lyases.

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Section: Antibiotic Applicationsmentioning

confidence: 92%

Section: Antibiotic Applicationsmentioning

confidence: 99%

Structure Characteristics, Biochemical Properties, and Pharmaceutical Applications of Alginate Lyases

et al. 2021

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“…To detect the minimum concentration inhibiting biofilm formation, we drained the wells' contents and washed the wells three times with physiological serum. In the next step, the 2, 3, 5-tri-phenyl-tetrazolium chloride (TTC) (Merck, Germany) staining method was used to observe the biofilms (27). In this method, the plates were dried under sterile conditions for 15 min, and then 170 mL of TSB culture medium and 30 mL of 2% TTC solution were added to each well.…”

Section: Determination Of Mbic By Broth Microdilution Methodsmentioning

confidence: 99%

“…Propolis also reduced the accumulation of human dental plaque and its insoluble external polysaccharide content (25,26). The ethanolic extract of propolis inhibits the growth of S. mutans, and its inhibitory effect is comparable to that of CHX against lactobacilli, P. intermedia, P. gingivalis, A. israelii, and Candida Albicans (20,27,28). However, its effects against oral pathogens have been compared with other oral antiseptics in a limited number of laboratory studies (29,30).…”

Section: Introductionmentioning

confidence: 99%

In vitro Evaluation of Synergistic Inhibitory Effect of Propolis and Chlorhexidine on Biofilm Cells of Oral Pathogens

Tavafi

2022

Jentashapir J Cell Mol Biol

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Background: Dental plaque is a microbial biofilm that can source various oral diseases. Objectives: This study aimed to investigate the synergistic anti-biofilm effect of chlorhexidine (CHX) and propolis aqueous extract on Streptococcus mutans and Staphylococcus aureus. Methods: This study, performed on October 2021, determined the minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC) of CHX 0.2% and propolis aqueous extract against S. mutans and S. aureus prepared from the cell bank of the Pasteur Institute of Iran. Also, the in vitro synergistic effect of these two compounds was evaluated on the biofilm inhibition and eradication of the studied bacteria using the checkerboard method. Results: The MBIC of chlorhexidine was 125 μg/mL for both S. aureus and S. mutans, while the MBIC of propolis aqueous extract was 937.5 and 3750 μg/mL, respectively. Also, the MBEC of chlorhexidine was 250 and ≥ 500 μg/mL for S. aureus and S. mutans, while the MBEC of propolis was 1875 and ≥ 7500 μg/mL, respectively. Combining the two substances showed a synergistic effect in inhibiting biofilm formation (P ≤ 0.05) but no significant difference in biofilm eradication. Conclusions: This study concluded that although CHX is one of the most common and vital oral disinfectants in oral diseases, propolis may also be used, as an alternative mouthwash with reliable natural antimicrobial properties, alone or combined with CHX as a moderator of its side effects.

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“…The alginate component in the biofilm of Pseudomonas aeruginosa is a key factor protecting them from being cleared by the immune system and antibiotic treatment (Maurice et al 2018;Wiens et al 2014). Therefore, it is possible to use purified alginate lyase with antibiotics to treat Pseudomonas aeruginosa infections (Daboor et al 2021;Tavafi et al 2018). A silver nanocomposite consisting of silver nanoparticles and a mesoporous organ silica layer was created to deliver two pharmaceutical compounds (alginate lyase and ceftazidime) to degrade the alginate and eradicate Pseudomonas aeruginosa from the lungs (Wan et al 2020).…”

Section: Alginate Lyase For Biomedicine Preparationmentioning

confidence: 99%

Evolving strategies for marine enzyme engineering: recent advances on the molecular modification of alginate lyase

Cao

et al. 2021

Mar Life Sci Technol

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Alginate, an acidic polysaccharide, is formed by β-d-mannuronate (M) and α-l-guluronate (G). As a type of polysaccharide lyase, alginate lyase can efficiently degrade alginate into alginate oligosaccharides, having potential applications in the food, medicine, and agriculture fields. However, the application of alginate lyase has been limited due to its low catalytic efficiency and poor temperature stability. In recent years, various structural features of alginate lyase have been determined, resulting in modification strategies that can increase the applicability of alginate lyase, making it important to summarize and discuss the current evidence. In this review, we summarized the structural features and catalytic mechanisms of alginate lyase. Molecular modification strategies, such as rational design, directed evolution, conserved domain recombination, and non-catalytic domain truncation, are also described in detail. Lastly, the application of alginate lyase is discussed. This comprehensive summary can inform future applications of alginate lyases.

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Screening, cloning and expression of a novel alginate lyase gene from P. aeruginosa TAG 48 and its antibiofilm effects on P. aeruginosa biofilm

Cited by 18 publications

References 32 publications

Structure Characteristics, Biochemical Properties, and Pharmaceutical Applications of Alginate Lyases

Structure Characteristics, Biochemical Properties, and Pharmaceutical Applications of Alginate Lyases

In vitro Evaluation of Synergistic Inhibitory Effect of Propolis and Chlorhexidine on Biofilm Cells of Oral Pathogens

Evolving strategies for marine enzyme engineering: recent advances on the molecular modification of alginate lyase

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