Screening, Classification and Distribution ofl-α-Amino-ε-caprolactam-hydrolyzing Yeasts

Hydrolysis of Amides

“…By combining this L-selective lactamase with the ACL racemase from the bacterium Achromobacter obae in one reaction vessel, DL-ACL is converted into L-lysine 40 in near quantitative yield (Scheme 15.6) [119]. The L-ACL lactamase containing C. laurantii strain was isolated from soil in a screening program for microorganisms that were able to grow on L-and DL-ACL but not on D-ACL as nitrogen source [120]. Owing to this enzymes high activity for L-ACL (240 mmol min À1 mg) À1 ) [121]) and near absolute stereoselectivity, DL-ACL (100 g l À1 ) was completely resolved by 500 mg of the lyophilized yeast cells in only 7 h. The e.e.…”

“…In a typical example (Scheme 15.24), carboxypeptidase Y (CPD-Y) catalyzed the kinetically driven coupling of H-Tyr-OEt (118) and H-Arg-NH 2 (119). The dipeptide amide formed (120) was then deamidated to HTyr-Arg-OH (121) by PAF without concurrent hydrolysis of the non-reacted Arg-NH 2 or the dipeptide, which significantly simplified downstream processing. Because of PAFs broad pH range (approx.…”

Biotechnology

“…In such a case, it becomes necessary to assay suitable microbial strains or enzymes as to their suitability for catalyzing the desired reaction. The production of l-lysine from d,l-α-amino-ε-caprolactam (ACL) is a typical example [296]. This process was designed to utilize cyclohexene, a major byproduct of nylon production.…”

Hydrolysis ofl-α-Amino-ε-caprolactam by Yeasts

“…Paper chromatography was performed using two kinds of solvents: (1) N-BuOH, CHaCOOH, H z O=4: 1: 2, (2) phenol, H z O=7: 3 and two kinds of location reagents: (1) The temperature-activity profiles of hydrolysis of L-aminolactam by the acetone-dried cells and the crude enzyme preparation of C. laurentii TORAY 2001 are shown in Fig. 2.…”