Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability

Song, ZhangYue; Tian, Jingluan; Fu, Weijun; Lin, Li; Lu, Linlin; Zhou, Lian; Zhang, Shan; Tang, Guixiang; Shou, Huixia

doi:10.1631/jzus.b1200278

Cited by 43 publications

(37 citation statements)

References 23 publications

(28 reference statements)

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“…The putative transgenic soybean plants were produced from in vitro culture of half-seed explants infected with Agrobacterium harboring binary vector pB7WG2D-SYNC1. Four lines of putative transgenic plants were produced, but the transformation efficiency was lower than the previous reports (Paz et al 2006;Song et al 2013). Furthermore, only one transgenic line (L3 line) transmitted to T 1 generation, while other lines failed to inherit the transgene to the next generation (Figure 4).…”

Section: Discussionmentioning

confidence: 87%

Asparaginyl-tRNA synthetase gene (SYNC1) characterized by Lotus corniculatus FOX-superroot lines has effects on plant morphology and amino acid contents of seed in soybean

Arifin

Gondo

Akashi

2019

Plant Biotechnology

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The application of useful genes from model plants to crops is an important step to verify its agricultural usefulness. SYNC1, an asparaginyl-tRNA synthetase gene, was previously identified through the Full-length cDNA Over-eXpressor gene (FOX gene-hunting system) of Arabidopsis cDNA by using super-growing root (SR) culture of Lotus corniculatus, and was suggested to have a potential in increasing some amino acid contents and plant biomass. To identify the functionality of SYNC1 gene in a typical legume crop soybean, the effects of its overexpression in transgenic plants to agricultural traits and free amino acid contents were evaluated. The transgenic soybean plants were produced from infected half-seed explants of 1 day old seedlings with the suspension of Agrobacterium tumefaciens harboring expression vector pB7WG2D-SYNC1. The transgenic plants that overexpressed SYNC1 gene had increased asparagine and lysine contents in matured seeds, and increased aspartate, lysine, alanine and histidine contents in germinated seeds. The changes in those free amino acid contents affected plant morphology and led to significant increase in plant length, number of branches and number of branch nodes as yield components of soybean. The transgenic plants also showed a tendency of higher number of pods, seeds and total seed weight per plant. These results showed that the overexpression of SYNC1 gene contributes on the increase of plant free amino acid contents and biomass, and this approach is expected to be applicable in other legumes, grain and forage crops.

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Section: Discussionmentioning

confidence: 87%

Asparaginyl-tRNA synthetase gene (SYNC1) characterized by Lotus corniculatus FOX-superroot lines has effects on plant morphology and amino acid contents of seed in soybean

Arifin

Gondo

Akashi

2019

Plant Biotechnology

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“…The above binary vectors were introduced into A. tumefaciens strain LBA4404. The vectors were transformed into soybean 'Williams 82' via A. tumefaciens as described by Song et al (2013).…”

Section: Plasmid Construction For Transformationmentioning

confidence: 99%

The Soybean Sugar Transporter GmSWEET15 Mediates Sucrose Export from Endosperm to Early Embryo

et al. 2019

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“…In this experiment, bar gene was used as an effective selectable marker for identification of the transgenic plants. This gene encodes phosphinothricin N-acetyltransferase (PAT) enzyme, which confers resistance to Basta herbicide containing glufosinate ammonium (De Block et al, 1987;Song et al, 2013). Due to the large number of transgenic plants that were used for screening soybean events, Basta application was chosen as a quick, cheap but accurate method.…”

Section: Successful Selection Of Homologous Transgenic Soybean Carryimentioning

confidence: 99%

PHYSIOLOGICAL ANALYSES OF AN OVER-EXPRESSING CYTOKININ METABOLIC GENE GmIPT10 UNDER NORMAL AND DROUGHT CONDITIONS

Hải¹,

Thien²,

Xuan³

et al. 2018

Vietnam J. Biotechnol.

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Cytokinin (CK) is an important phytohormone, which not only plays significant role in plant development but also involves in mediating plant stress tolerance. Previous studies showed that the drought tolerance can be improved by stress-inducible overexpression of adenine isopentenyl transferase (IPT), which is a critical enzyme in CKs biosynthesis. To study the role of soybean GmIPT10 in regulating plant tolerance, we successfully generated GmIPT10-overexpressing transgenic soybean plants and screened a line carrying homozygous, single copy of transgene. Analyzing several physiological traits of this line demonstrated that it possessed stress tolerance characteristics, including increased primary root and shoot lengths, better production of shoot biomass, higher number of trifoliate leaves, and higher survival rate than the non-transgenic plants under drought condition. The net house experiments also showed that the GmIPT10-overexpressing transgenic soybean had a greater relative water content compared to the control genotype under applied drought condition. Therefore, this report indicated that plant drought tolerance might be enhanced via regulating expression of GmIPT10.

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Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability

Cited by 43 publications

References 23 publications

Asparaginyl-tRNA synthetase gene (<i>SYNC1</i>) characterized by <i>Lotus corniculatus</i> FOX-superroot lines has effects on plant morphology and amino acid contents of seed in soybean

Asparaginyl-tRNA synthetase gene (<i>SYNC1</i>) characterized by <i>Lotus corniculatus</i> FOX-superroot lines has effects on plant morphology and amino acid contents of seed in soybean

The Soybean Sugar Transporter GmSWEET15 Mediates Sucrose Export from Endosperm to Early Embryo

PHYSIOLOGICAL ANALYSES OF AN OVER-EXPRESSING CYTOKININ METABOLIC GENE GmIPT10 UNDER NORMAL AND DROUGHT CONDITIONS

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