Screening bacterial phosphate solubilization with bulk-tricalcium phosphate and hydroxyapatite nanoparticles

Miguel, Rosalba Monroy; Carrillo-González, Rogelio; Leal, Elvira Rios; González-Chávez, Ma. del Carmen A.

doi:10.1007/s10482-020-01409-2

Cited by 8 publications

(5 citation statements)

References 25 publications

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“…At 21 days, only 39B isolate was found to enhance significantly its growth with a concomitant increase in the soluble-P content with NO 3 as the sole N-source. This result would be in line with the previous evidences reported by [33,34] who found that mechanisms of P-solubilization depend on processes associated with microbial biomass production (i.e. NH 4 + -assimilation or respiration).…”

Section: Plos Onesupporting

confidence: 93%

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Improved rock phosphate dissolution from organic acids is driven by nitrate assimilation of bacteria isolated from nitrate and CaCO3-rich soil

et al. 2023

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Until now, the solubilization capacities of insoluble mineral P by soil microorganisms have been screened in vitro with media containing NH4+ as a nitrogen source. This presence of NH4+ will lead to an acidification of the medium responsible for the solubilization of the insoluble P. However, besides proton release, the production of organic acids can play a very important role in the release of free P. This physiological mechanism can largely depend on the source of nitrogen (NH4+vs NO3-) assimilated by the bacteria but the influence of the N source on the production of organic acids has yet to be studied. Our aim was to investigate if the N source assimilated by bacteria and the soil characteristics such as the dominant N source (NH4+vs NO3-) and CaCO3 contents might influence the bacterial capacities to solubilize rock phosphate. To fill this objective, we screened the capacity of bacteria isolated from 3 soils to solubilize rock phosphate in vitro in presence of NH4+or NO3-. Then, we selected the most efficient bacterial strains to identify and quantify the release of organic anions into the medium. Among the two hundred and forty-three bacterial strains isolated from the 3 soils, nine and seven isolates were identified with the highest % rock phosphate-solubilization values with NH4+ or NO3- as the sole N-source. Only one strain was able to release free Pi with NH4+ or NO3- as the sole N-source. The most predominant organic acids released by almost all isolates were gluconic acid, lactic acid, glycolic acid, acetic acid, formic acid and pyruvic acid regardless the N-source. However, with NO3- as source of N, the highest concentrations on those acids were found together with the highest release of free Pi into the medium. Molecular analysis of 16S rRNA indicated that almost all strains belonged to Bacillus and Paenibacillus genera. The PCA analysis between soil properties and bacterial capacities to release organic acids and free Pi also revealed that soil factors such as CaCO3 and soil NO3- content positively influenced the release of organic acids by bacteria grown in vitro. Our results concluded that the bacterial rock phosphate-solubilization was intimately related to organic acids production which in turn seemed to be driven by the assimilation of NO3- by bacteria. Therefore, the N-source might be considered a key factor to take into consideration during the screening and selection of suitable strains involved in the P-solubilization.

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Section: Plos Onesupporting

confidence: 93%

“…P-sources instead of spending bacterial sources to biomass production [34]. However, the pH drop found for all isolates after 3 days of culture would negatively affect the bacterial growth.…”

Section: Plos Onementioning

confidence: 90%

Improved rock phosphate dissolution from organic acids is driven by nitrate assimilation of bacteria isolated from nitrate and CaCO3-rich soil

et al. 2023

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“…Other organic acids were also produced by JP233 including (2-methoxyethoxy) propanoic acid, 2-oxopentanedioic acid, D-(+)-pantothenic acid, N-acetyl-L-glutamic acid, trans-aconitic acid, succinic acid, 3-oxopentanoic acid, maleic acid, pantothenic acid, and 2-ketohexanoic acid. Acids released by PSB that have been shown to be involved in P solubilization include citric acid, formic acid, gluconic acid, 2-keto gluconic acid, lactic acid, malic acid, oxalic acid, propionic acid, and succinic acid (Khan et al, 2007;Sharma et al, 2013;Alori et al, 2017;Chakdar et al, 2018;Su et al, 2019;Monroy Miguel et al, 2020;Spohn et al, 2020). It should be noted that organic acids produced by strains of the genus are not identical.…”

Section: Discussionmentioning

confidence: 99%

Identification of the Phosphorus-Solubilizing Bacteria Strain JP233 and Its Effects on Soil Phosphorus Leaching Loss and Crop Growth

Zhang

et al. 2022

Front. Microbiol.

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Phosphorus (P) is one of the most limiting nutrients in global agricultural ecosystems, and phosphorus-solubilizing bacteria (PSB) can convert insoluble P into soluble P, thereby improving the absorption and use of soil P by plants. Increasing leaching loss of soil P due to PSB that could lead to water eutrophication is a major concern, although no direct experimental evidence is available to evaluate these effects. In this study, a highly efficient PSB strain, Pseudomonas sp. JP233, was isolated from soil and its P-solubilizing agent was identified by metabolomics and HPLC analyses. The effects of JP233 on P contents in soil leachates were also analyzed by microcosm leaching experiments in the absence and presence of maize. JP233 could solubilize insoluble P into soluble forms, and the molybdate reactive phosphorus (MRP) content reached 258.07 mg/L in NBRIP medium containing 5 g/L Ca3(PO4)2 within 48 h. Metabolomics analysis demonstrated that the organic acid involved in JP233 P solubilization was primarily 2-keto gluconic acid (2KGA). Further, HPLC analysis revealed that 2KGA contents rapidly accumulated to 19.33 mg/mL within 48 h. Microcosm leaching experiments showed that MRP and total phosphorus (TP) contents in soil leaching solutions were not significantly higher after JP233 inoculation. However, inoculation with JP233 into maize plant soils significantly decreased MRP and TP contents in the soil leaching solutions on days 14 (P < 0.01), 21 (P < 0.01), and 28 (P < 0.05). Inoculation with strain JP233 also significantly increased the biomass of maize aerial components and that of whole plants (P < 0.05). Thus, strain JP233 exhibited a significant plant-growth-promoting effect on maize development. In conclusion, the application of PSB into soils does not significantly increase P leachate loss. Rather, the application of PSB can help reduce P leachate loss, while significantly promoting plant absorption and use of soil P.

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“…However, most of the experiments were conducted on culturable microorganisms or soil extracts. For instance, in culture media, P was shown to be solubilized from apatite (Amy et al, 2022; Kim et al, 1997; Monroy Miguel et al, 2020), from phytate (organic P) (Amy et al, 2022; Shulse et al, 2019) and from Fe phosphates (He et al, 2007; Jha et al, 2013). Similarly, tests were conducted on soil extracts in which the solubilization from apatite (Brucker et al, 2020; Efthymiou et al, 2018; Pastore, Kernchen, & Spohn, 2020) and Fe phosphates (Efthymiou et al, 2018; Pastore, Kaiser, et al, 2020) was assessed.…”

Section: Introductionmentioning

confidence: 99%

Soil microorganisms increase Olsen phosphorus from poorly soluble organic phosphate: A soil incubation study

Velasco-Sánchez

Bennegadi-Laurent

Trinsoutrot‐Gattin

et al. 2023

Soil Use and Management

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The potential shortage of mineral phosphorus (P) sources and the shift towards a circular economy motivates the introduction of new forms of P fertilizers in agriculture. However, the solubility of P in new fertilizers as well as their availability to plants may be low. In this experiment, we incubated an agricultural soil poor in P (28 mg P2O5 kg‐1) for 63 days in the presence of a range of organic and inorganic poorly soluble P forms commonly found in new fertilisers: hydroxyapatite (P‐Ca), iron phosphate (P‐Fe), phytic acid (P‐Org) and a combination of P‐Ca and P‐Org (P‐Mix). Cellulose and potassium nitrate (KNO3) were added to stimulate microbial activity at the beginning of the incubation. We included a positive control with triple superphosphate (TSP) and negative controls with no P application (with and without cellulose and KNO3). We assessed the fate of the different poorly soluble P forms in NaHCO3 extracts (Olsen P) over time as a proxy for plant available P. Soil microbial biomass, fungal to bacterial ratio, soil respiration, enzymatic activities (β‐glucosidase, arylamidase and acid and alkaline phosphatase), N mineralisation and soil pH were also monitored. At the beginning of the incubation, TSP showed the highest Olsen P across all treatments and P‐Fe showed higher levels of Olsen P than the other poorly soluble P forms (p < 0.05). During the incubation, the levels of Olsen P decreased over time for TSP (positive control). Contrastingly, Olsen P increased significantly over time for all the poorly soluble P forms and the negative controls, indicating an increase in plant available P. Particularly, levels of Olsen P for the P‐Org treatment roughly doubled (shifting from 16.5 mg kg‐1 to 32.9 mg kg‐1) over the whole incubation period. The rate of increase in Olsen P was positively correlated with microbial biomass C: P ratio (p < 0.01) for all poorly soluble treatments. The higher levels of Olsen P for the P‐Org treatment were also explained by a positive correlation with fungal biomass. Our results show that poorly soluble forms of P may be made available to plants under the influence of the microbial community, with a stronger effect for organic P forms.

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Screening bacterial phosphate solubilization with bulk-tricalcium phosphate and hydroxyapatite nanoparticles

Cited by 8 publications

References 25 publications

Improved rock phosphate dissolution from organic acids is driven by nitrate assimilation of bacteria isolated from nitrate and CaCO3-rich soil

Improved rock phosphate dissolution from organic acids is driven by nitrate assimilation of bacteria isolated from nitrate and CaCO3-rich soil

Identification of the Phosphorus-Solubilizing Bacteria Strain JP233 and Its Effects on Soil Phosphorus Leaching Loss and Crop Growth

Soil microorganisms increase Olsen phosphorus from poorly soluble organic phosphate: A soil incubation study

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