Screening and Xylanase Production by Streptomyces sp. Grown on Lignocellulosic Wastes

Abstract

Xylanases have raised interest because of their potential applications in various industrial fields, including the pulp and paper industries, bioethanol production, and the feed industry. In bioethanol production from lignocellulosic compounds, xylanase can improve the hydrolysis of cellulose into fermentable sugars, since the xylan restricts the cellulases from acting efficiently. In this work, a new thermophilic Streptomyces sp. was selected for its ability to produce xylanase. Carbon source selection is an important factor in the production of hemicellulases. The highest activity was obtained when Streptomyces sp. I3 was grown in the presence of wheat bran. Xylanase activity was partially characterized concerning the effect of pH and temperature on activity and thermostability, and the effects of different metal ions were also tested. The pH and temperature profile showed optimal activity at pH 6.0/70 °C. Zymogram analysis showed multiple xylanases (39, 21, 18, and 17 kDa). Xylanases studied in this work are thermophilic, thermostable, and active in a wide pH range; they have potential to be used in the development of new processes of biotechnological interest.

Highly Thermostable Xylanase Production from A Thermophilic Geobacillus sp. Strain WSUCF1 Utilizing Lignocellulosic Biomass

“…On the other hand, perennial grasses like prairie cordgrass is also considered as one of the most abundant biomass feedstocks in the Great Plains (Gonzalez-Hernandez et al, 2009; Rastogi et al, 2010; Zambare et al, 2011). Brito-Cunha et al (2013) reported high xylanases production utilizing sugarcane bagasse and wheat bran as substrates from Streptomyces sp. Thermophilic xylanolytic Thermoanaerobacter strains have also been reported to produce xylanases with poplar, spruce, miscanthus, wheat straw, whole corn plants, corn cobs, corn stalks, sugarcane bagasse, sweet sorghum, or cotton stalks (Svetlitchnyi et al, 2013).…”

Streptomyces thermocerradoensis I3 secretes a novel bifunctional xylanase/endoglucanase under solid‐state fermentation

“…The influence of ions on the activities of enzymes present in the F3 was studied by incubating mixtures of 90 μl of F3 with 10 μl of 5 mM ion solutions (metals: aluminum, barium, calcium, potassium, magnesium, sodium, manganese, copper, lithium, mercury, silver, zinc, and iron; nonmetals: ammonia in the chloride form) at 50 C for 1 hr. 12 The influence of ethylenediamine tetraacetic acid (EDTA), sodium dodecyl sulfate (SDS), and 2-mercaptoethanol was also analyzed under the same conditions. 16 The enzymatic activities of these samples were subsequently analyzed and the data were subjected to analysis of variance (ANOVA) followed by Dunnett test in the Statistica 8.0 software.…”

“…Because most xylanases have low molecular weights, the F3 was selected for better characterization. 12,20 The protein profile of selected fractions is shown in Figure 3. Surprisingly, SDS-PAGE showed a single band.…”

“…The results revealed the production of at least four xylanases. The presence of multiple xylanases is relatively common for the genus Streptomyces . Many of the characterized xylanases belong to the glycoside hydrolase (GH) families 10 and 11.…”

“…The presence of multiple xylanases is relatively common for the genus Streptomyces. 12 Many of the characterized xylanases belong to the glycoside hydrolase (GH) families 10 and 11. The members of GH 11 are called true xylanases owing to their stringent substrate specificity.…”