Screening and selection of autochthonous fungi from leachate contaminated-soil for bioremediation of different types of leachate

Abstract

This study aims to use contaminated soil with leachate to select autochthonous fungi that are able to bioremediate three types of leachate, (Young (YL), Intermediate (IL) and Old (OL)). Eleven fungal species were isolated via the enrichment method using the leachate as the sole source of carbon and energy. The isolates were evaluated for their ability to grow and remove organic pollutants at 100%, 50% and 25% (v/v) of leachate in both solid and liquid cultures that were spiked with malt extract. The results indicated that only three fungi, <i>Aspergillus flavus</i> (<i>A. flavus</i>-LC106118), <i>Aspergillus niger</i> (<i>A. niger</i>-KT192262) and <i>Fusarium solani</i> (<i>F. solani</i>-KX349467) showed significantly high capacity to grow on leachate, with maximum radial growth rates (Gr) of 7.5 mm, 4.7 mm , and 5.3 mm, respectively. In addition, 34%, 22%, and 27%, respectively of COD removal rates were obtained at 25% concentration in YL. A. flavus was the most tolerant fungus against landfill leachate, followed by <i>F. solani</i>, and finally <i>A. niger</i>. Therefore, these three fungi are good candidates for leachate bioremediation. However, for a better remediation, the combined effects of different types of fungi and leachates on the fungal growth need to be considered during the fungi selection.

DNA metabarcoding of the leachate microbiota from sanitary landfill: potential for bioremediation process

“…Although toxic contaminants in leachate, including dissolved organic matter, heavy metals and organic xenobiotic compounds may have in uenced the microbial composition in the samples studied [51]. Our study is essential, since it was possible to observe a considerable microbial community related to pathogenic taxonomic groups, indicating care with potential leaks and environmental contamination.…”

Experimental Observations and Assessment of Landfill Leachate Bioremediation by Autochthonous Fungi Species and their Effective Geoactivities

“…The isolated fungi were identified following the atlases of mycology and previous The statistical analysis, Figure 3, shows 47% of the isolates from Soran City landfill sites belong to the genus of Aspergillus whose frequency was 72 and contained different species such as Aspergillus niger, Aspergillus fumigatus, Aspergillus terreus, Aspergillus flavus, Aspergillus pseudoelegans, and Aspergillus elegans, and 16% and 12% were species of Penicillium and Rhizopus whose frequencies were 25 and 19, respectively. Other studies have shown that aspergillus and penicillium were the dominating species in landfills (Zegzouti et al, 2020). The species of penicillium were divided into three different species.…”

Bioremediation of Soil Pollution Based on Fungal Immobilization Technology

“…A suitable carrier can protect the microorganisms from the external environment as much as possible, and can also reduce the loss of microorganisms during immobilization. At present, the most widely used materials for immobilized microorganisms are divided into inorganic materials, natural organic polymer materials, synthetic organic polymer materials and composite materials [5][6].…”