Screening and Phenotypic Diversity of Amylase Producing Rhizospheric Bacteria from Some North African Plants

Choubane, Slimane; Cheba, Ben Amar; Benourrad, Amina

doi:10.1016/j.protcy.2016.01.168

Cited by 20 publications

(12 citation statements)

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“…Many plant beneficial bacteria produces extracellular enzymes through indirect mechanism for PGP (Gupta et al, 2015). The production of hydrolytic enzymes by EnB emerges as important for colonization of plant roots and movement of bacteria into the interior of plants rendering help in plant growth (Al-Mallah et al, 1987;Quadt-Hallmann et al, 1997;Choubane et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Prospecting Endophytic Bacteria Endowed With Plant Growth Promoting Potential Isolated From Camellia sinensis

et al. 2021

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Endophytes are well-acknowledged inoculants to promote plant growth, and extensive research has been done in different plants. However, there is a lacuna about the endophytes associated with tea clones and their benefit to promote plant growth. The present study focuses on isolating and characterizing the beneficial endophytic bacteria (EnB) prevalent in commercially important tea clones cultivated in North Eastern India as plant growth promoters. Diversity of culturable EnB microbiome, in vitro traits for plant growth promotion (PGP), and applicability of potent isolates as bioinoculant for in vivo PGP abilities have been assessed in the present study. A total of 106 EnB identified as members of phyla Proteobacteria, Firmicutes, and Actinobacteria were related to 22 different genera and six major clusters. Regarding PGP traits, the percentage of isolates positive for the production of indole acetic acid, phosphate solubilization, nitrogen fixation siderophore, ammonia, and 1-aminocyclopropane-1-carboxylic acid deaminase production were 86.8, 28.3, 78.3, 30.2, 95.3, and 87.7, respectively. In total, 34.0, 52.8, and 17.0% of EnB showed notable production of hydrolytic enzymes like cellulase, protease, and amylase, respectively. Additionally, based on the bonitur score, the top two isolates K96 identified as Stenotrophomonas sp. and M45 identified as Pseudomonas sp. were evaluated for biofilm formation, motility, and in vivo plant growth promoting activity. Results suggested strong biofilm formation and motility in K96 and M45 which may attribute to the colonization of the strains in the plants. Further in vivo plant growth promotion experiment suggested sturdy efficacy of the K96 and M45 as plant growth promoters in nursery condition in commercial tea clones Tocklai vegetative (TV) TV22 and TV26. Thus, this study emphasizes the opportunity of commercialization of the selected isolates for sustainable development of tea and other crops.

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Section: Discussionmentioning

confidence: 99%

Prospecting Endophytic Bacteria Endowed With Plant Growth Promoting Potential Isolated From Camellia sinensis

et al. 2021

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“…In a recent study, Mardanova et al [52] reported similar findings for both antagonistic bacteria B. subtilis GM2 and B. subtilis GM5. However, the α-amylase was considered in several studies as an enzyme with a minor role in the control of certain fungi [53].…”

Section: Discussionmentioning

confidence: 99%

The Potential of Novel Bacterial Isolates from Natural Soil for the Control of Brown Rot Disease (Monilinia fructigena) on Apple Fruits

et al. 2020

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Monilinia fructigena is one of the most important fungal pathogens causing brown rot on apple and is heavily affecting fruit production. The main objective of this study was to screen for potential bacterial isolates with higher antagonistic activity against M. fructigena. Our study focused on the identification of potential bacterial isolates capable of reducing both the mycelial growth of M. fructigena and the disease severity using in vitro and in planta trials, respectively. To achieve this goal, thirteen bacteria, isolated from natural soil, were evaluated for their abilities to produce lytic enzymes (amylase, cellulase and protease), hydrocyanic acid (HCN) and lipopeptides (bacillomycin, fengycin, iturin and surfactin). Further, results from the dual culture method, volatile and bacterial free-cell filtrate bioassays indicated that tested isolates showed a fungicidal activity against the mycelial growth of M. fructigena. Thus, out of the 13 isolates tested, 12 exhibited significant mycelial inhibition (more than 70%) against M. fructigena, while remaining the last isolate displayed only a partial inhibition (up to 43%). Further, 12 of the bacteria isolates displayed an amylase production, 10 showed cellulase production, 11 revealed protease production, while only 2 displayed HCN production. In addition, most bacterial isolates were found to have genes encoding for different lipopeptides: bacillomycin (10), fengycin (3), iturin (11) and surfactin (1). Interestingly, two bacterial isolates, Bacillus amyloliquefaciens B10W10 and Pseudomonas sp. B11W11 were found to be the most effective and displayed the lowest disease severity in planta trial. These two bacteria reduced the brown rot incidence compared to the synthetic fungicide in a semi-commercial large-scale trial. Therefore, our findings suggest that these two later bacterial isolates provide apple protection against M. fructigena via direct and indirect mechanisms. These isolates may be used, therefore, as potential biological control agents (BCAs) in preventive treatment to control brown rot disease on apple fruits.

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“…Regarding the production of hydrolytic enzymes, ∼58% of the isolates were amylase producers, 63% cellulases and 47.69% proteases. The secretion of these enzymes in the environment can play a fundamental role in plant growth by promoting the cycling of organic matter in the soil ( Choubane et al, 2016 ). In the case of canga soils, which are reported to have low fertility, these isolates may be of crucial importance in the cycling of essential nutrients for the maintenance of the plants.…”

Section: Discussionmentioning

confidence: 99%

Brazilian Ironstone Plant Communities as Reservoirs of Culturable Bacteria With Diverse Biotechnological Potential

et al. 2018

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Extensive mineral extractivism in the Brazilian Iron Quadrangle (IQ) region has destroyed large areas of land, decimating plant species, and their associated microbiota. Very little is known about the microbiota of the region; hence, cultivable bacteria associated with plants of its soils were investigated for their biotechnological potential. Samples were collected from nine plant species and six soils, and 65 cultivable bacterial isolates were obtained. These represent predominantly gram-positive bacilli (70%) capable of producing amylases (55%), proteases (63%), cellulases (47%), indole acetic acid (IAA) (46%), siderophores (26%), and to solubilize phosphate (9%). In addition, 65% of these were resistant to ampicillin, 100% were sensitive to tetracycline, and 97% were tolerant to high arsenic concentrations. Three isolates were studied further: the isolate FOB3 (Rosenbergiella sp.) produced high concentrations of IAA in vitro in the absence of tryptophan – shown by the significant improvement in plant germination and growth rate where the isolate was present. For isolates C25 (Acinetobacter sp.) and FG3 (Serratia sp.), plasmids were purified and inserted into Escherichia coli cells where they modified the physiological profile of the transformed strains. The E. coli::pFG3B strain showed the highest capacity for biofilm production, as well as an increase in the replication rate, arsenic tolerance and catalase activity. Moreover, this strain increased DNA integrity in the presence of arsenic, compared to the wild-type strain. These results help to explain the importance of bacteria in maintaining plant survival in ferruginous, rocky soils, acting as plant growth promoters, and to highlight the biotechnological potential of these bacteria.IMPORTANCE The Iron Quadrangle region is responsible for ∼60% of all Brazilian iron production and, at the same time, is responsible for housing a wide diversity of landscapes, and consequently, a series of endemic plant species and dozens of rare species – all of which have been poorly studied. Studies exploring the microbiota associated with these plant species are limited and in the face of the continuous pressure of extractive action, some species along with their microbiota are being decimated. To understand the potential of this microbiota, we discovered that cultivable bacterial isolates obtained from plants in the ferruginous rocky soil of the Iron Quadrangle region have diverse biotechnological potential, revealing a genetic ancestry still unknown.

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Screening and Phenotypic Diversity of Amylase Producing Rhizospheric Bacteria from Some North African Plants

Cited by 20 publications

References 12 publications

Prospecting Endophytic Bacteria Endowed With Plant Growth Promoting Potential Isolated From Camellia sinensis

Prospecting Endophytic Bacteria Endowed With Plant Growth Promoting Potential Isolated From Camellia sinensis

The Potential of Novel Bacterial Isolates from Natural Soil for the Control of Brown Rot Disease (Monilinia fructigena) on Apple Fruits

Brazilian Ironstone Plant Communities as Reservoirs of Culturable Bacteria With Diverse Biotechnological Potential

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