Screening and monitoring antiretrovirals and antivirals in the serum of acquired immunodeficiency syndrome patients by micellar liquid chromatography

Raviolo, Mónica A.; Breva, Inmaculada Casas; Esteve‐Romero, Josep

doi:10.1016/j.chroma.2009.01.082

Cited by 18 publications

(35 citation statements)

References 33 publications

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“…12 We have previously developed a methodology based on micellar liquid chromatography for the screening of 13 antiretroviral drugs by the use of only three mobile phases. 6 The use of micellar solvents is especially useful in samples with serum matrices, because serum contains hydrophobic compounds which are harmful for the chromatographic system. [13][14][15] Micellar media denaturalize and solubilize hydrophobic substances, releasing possible interferent-analyte adducts and avoiding precipitation into the columns.…”

Section: 9mentioning

confidence: 99%

“…8,16 After a simple dilution and filtration, samples are able to be directly injected, expediting the experimental procedure. 17 Our previously developed analytical method 6 can be adapted by the modification of the mobile phases in order to analyze three determined antiretrovirals. This way, this modified methodology will keep the benefits of micellar liquid chromatography, including the use of a low amount of pollutant chemicals.…”

Section: 9mentioning

confidence: 99%

“…5 HAART involves different kinds of antiretroviral drugs, such as: nucleoside reverse transcriptase inhibitors (NRTIs): abacavir, didanosine, emtricitabine, lamivudine, stavudine, tenofovir, zalcitabine and zidovudine; non-nucleoside reverse transcriptase inhibitors (NNRTIs): delavirdine, nevirapine and efavirenz; and protease inhibitors (PIs): amprenavir, atazanavir, darunavir, fosamprenavir, indinavir, lopinavir, nelfinavir, ritonavir, saquinavir and tipranavir. 6,7 HAART regimes contain three or four drugs, two NRTIs and one or two NNRTIs, or one or two PIs. The hydrophobicity is assessed by the quotient of the solubility in octanol and in water (P o/w ).…”

Section: Introductionmentioning

confidence: 99%

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Monitoring of HAART regime antiretrovirals in serum of acquired immunodeficiency syndrome patients by micellar liquid chromatography

Casas-Breva¹,

Péris-Vicente

Rambla-Alegre

et al. 2012

Analyst

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A methodology based on micellar liquid chromatography to monitor five antiretroviral drugs (lamivudine, stavudine, tenofovir, zidovudine and efavirenz) was proposed. Antiretrovirals were studied in sets of three, corresponding to each highly active antiretroviral therapy (HAART) regime, prescribed to acquired immunodeficiency syndrome (AIDS)-infected patients. Four aqueous micellar mobile phases buffered at pH 7 were optimized to separate these compounds, using sodium dodecyl sulfate as the tensioactive, and 1-propanol or 1-pentanol as the organic modifier. The composition of each mobile phase was optimized for each antiretroviral. The common separation conditions were: C18 apolar column (125 Â 4.6 mm, 5 mm particle size), UV detection set at 214 nm, and mobile phase running at 1 mL min À1 without controlling the temperature. The finally suggested method was validated for five analysed antiretroviral drugs following the US Food and Drug Administration guidelines in terms of: linearity between 0.5 and 50 ppm (r 2 > 0.9995), sensitivity (LOD lower than 0.25 ppm), intra-and inter-day precision (<7.1 and <5.2%, respectively) and accuracy (recovery 88.5-105.3% and 93.5-101.3%, respectively), as well as robustness (<6.5%). The proposed method was used to monitor the level of antiretrovirals in the serum of AIDS patients. The suggested methodology was found to be useful in the routine analysis of antiretrovirals in serum samples.

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Section: 9mentioning

confidence: 99%

Section: 9mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Monitoring of HAART regime antiretrovirals in serum of acquired immunodeficiency syndrome patients by micellar liquid chromatography

Casas-Breva¹,

Péris-Vicente

Rambla-Alegre

et al. 2012

Analyst

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“…The purpose of this article is to provide a survey of the literature concerning the application of MLC in the analysis of the antitumoral drug tamoxifen (TAMO) and its metabolite endoxifen (ENDO) [4,33] and the antiretroviral drugs used in highly active antiretroviral therapy (HAART) [34,35] in plasma. In the first part of the article, the strategies for optimizing the experimental procedure and chromatographic parameters in MLC are detailed.…”

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confidence: 99%

Application of Micellar Liquid Chromatography for The Determination of Antitumoral and Antiretroviral Drugs in Plasma

Péris-Vicente

Casas-Breva²,

Roca-Genovés

et al. 2014

Bioanalysis

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In micellar liquid chromatography, the mobile phase is made of a surfactant and, eventually, an alcohol. This article describes several methods to measure the concentration of antitumoral and antiretroviral drugs in plasma, utilizing micellar liquid chromatography. Samples can be injected after dilution with a micellar solution and filtration, because proteins and other endogenous compounds are solubilized in micellar medium. We will discuss the following optimized parameters: dilution ratio, type of column, detection conditions and mobile phase composition. This article will also cover the validation performed following the International Conference on Harmonization guidelines and the results reported in the literature, indicating that the methods are useful for the routine analysis of plasma samples for clinical purposes.

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“…1). In literature, a number of analytical methods have been described for estimation of AVS including electrochemical determination 1 , HPLC 2,3 , LC 4,5 and LC-MS 6,7 , and UV [8][9][10] methods. But, relatively little attention has been paid in developing visible spectrophotometric methods [11][12][13][14][15] .The analytically important functional groups in AVS have not been exploited properly so far and hence there is a need to develop sensitive and flexible visible spectrophotometric methods.…”

mentioning

confidence: 99%

Quantitative Determination of HIV-Antiviral Agent using Complexing Reagent in Bulk Material and Formulations

Sastry¹,

Ramakrishna²,

Lavanya³

et al. 2014

Orient. J. Chem

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Screening and monitoring antiretrovirals and antivirals in the serum of acquired immunodeficiency syndrome patients by micellar liquid chromatography

Cited by 18 publications

References 33 publications

Monitoring of HAART regime antiretrovirals in serum of acquired immunodeficiency syndrome patients by micellar liquid chromatography

Monitoring of HAART regime antiretrovirals in serum of acquired immunodeficiency syndrome patients by micellar liquid chromatography

Application of Micellar Liquid Chromatography for The Determination of Antitumoral and Antiretroviral Drugs in Plasma

Quantitative Determination of HIV-Antiviral Agent using Complexing Reagent in Bulk Material and Formulations

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