Screening and isolation of a cellulolytic and amylolytic Bacillus from sago pith waste.

Apun, Kasing; Jong, Bor Chyan; Salleh, Mohd Azib

doi:10.2323/jgam.46.263

Cited by 83 publications

(65 citation statements)

References 14 publications

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“…The data presented in Table 1, showed that twenty isolates were selected. Screening of all isolates was conducted by using the congo red test (Apun et al 2000) as a preliminary study to select cellulase producers.…”

Section: Resultsmentioning

confidence: 99%

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culture conditions for production of cellulase by Aspergillus tubingensis KY615746 using rice straw waste

El-Nahrawy

Metwally

El-Kodoos

et al. 2017

EBSS

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TWENTY isolates of cellulase producing fungi were isolated from six samples of rotted rice straw which collected from different localities in El-Gharbia Governorate (Basyion), Kafrelsheikh Governorate (Sakha) and El-Dakahlia Governorate (El-Mansoura). On the basis of hydrolysis zone surrounding the colonies, an efficient fungus isolate (F7) for highest cellulolytic activity were chosen. According to morphological and biochemical characteristics as well as 18S rRNA sequence indicated that the isolate belonged to Aspergillus tubingensis KY615746. Effect of culture conditions for production of cellulase enzyme by A. tubingensis were investigated under Submerged Fermentation (SMF) and Solid State Fermentation (SSF) techniques. The optimum conditions for cellulase production by A. tubingensis were the best inoculum size was found to be 3%, pH 4, temperature 30 °C and incubation period 6th day where the reducing sugars attained (0.675, 0.728, 0.731 and 0.913 mg ml -1 ) with maximum activity (0.050, 0.055, 0.053 and 0.063 U ml -1 ), respectively for SMF conditions.On the other hand, the maximum cellulase activity under SSF technique with moisture level 10g : 30ml were (0.16 Ug -), incubation period 9 th day, inoculum size 4 ml:10g, pH 5 and temperature 30 °C where the reducing sugars were (1.412, 1.532, 1.551 and 1.521 mg g -1 ) with maximum activity (0.20, 0.22, 0.23 and 0.23 Ug -1 ), respectively. Based on the results, it may be concluded that rice straw waste can be a potential substrate for produce cellulase by SSF technique.

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Section: Resultsmentioning

confidence: 99%

“…The plates were stained with 0.1% (w/v) congo red dye for 15 min followed by destaining with 1 M NaCl solution for 15 to 20 min. Apun et al (2000); unstained areas (clear zone of hydrolysis) indicate where the CMC was hydrolysed.…”

Section: Culture Media and Growth Conditionsmentioning

confidence: 99%

culture conditions for production of cellulase by Aspergillus tubingensis KY615746 using rice straw waste

El-Nahrawy

Metwally

El-Kodoos

et al. 2017

EBSS

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“…To isolate cellulolytic bacteria, aliquots from various dilutions (10 -3 -10 -6 ) were plated in duplicate on carboxylmethyl cellulose (CMC) agar [20]. The CMC agar comprising (g/L) CMC (SigmaAldrich, Germany), 5; NaNO 3 (Lab M, India), 1; K 2 HPO 4 (Applichem, Germany), 1; KCl (Lab M, UK), 1; MgSO 4 (Sigma-Aldrich, Germany), 0.5; yeast extract (Sigma-Aldrich, Germany), 0.5; glucose (Oxoid, UK), 1 and Agar (Sigma-Aldrich, Germany), 17) was prepared by dissolving the ingredients in 1 L distilled water.…”

Section: Isolation and Screening Of Cellulolytic Bacteriamentioning

confidence: 99%

“…Lignin: The residue that remained after determination of cellulose was treated with 25 ml KMnO 4 buffer for 90 min at [20][21][22][23][24][25] o C. Lignin was dissolved leaving cutin and silica as insoluble materials. The contents were then filtered through tarred sintered crucible using gentle suction and residue was washed with distilled water, then with acetone.…”

Section: Hemicellulose (%) = Ndf (%)-Adf (%)mentioning

confidence: 99%

Bioethanol Production by an Ethanol-Tolerant Bacillus cereus Strain GBPS9 Using Sugarcane Bagasse and Cassava Peels as Feedstocks

Ezebuiro¹,

Ogugbue²,

Oruwari³

et al. 2015

J Biotechnol Biomater

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Bioethanol production potential of ethanol-tolerant Bacillus cereus strain GBPS9 using sugarcane bagasse and cassava peels as feedstocks was investigated. The Bacillus cereus GBPS9 used in this study was isolated from agro-wastes impacted soil and classified based on phylogenetic analysis of its 16S rRNA gene. The sequence of the isolate has been deposited in GenBank under the accession number KT318371.1. The isolate was selected based on its cellulolytic ability, tolerance to ethanol concentration of 6% (v/v) and ability to ferment sugar to ethanol. The substrates employed in the study were cassava peels and sugarcane bagasse. Chemical composition analysis showed total carbohydrate and lignin contents (% dry weight) of 69.6 ± 1.2 and 13.9 ± 0.4 for cassava peels and 70.3 ± 1.9 and 16.2 ± 1.2 for sugarcane bagasse, respectively. The feedstocks were subjected to acid, alkali and steam explosion pretreatments to increase cellulose content and therefore, reduce lignin content. The best pretreatment methods (steam explosion for sugarcane bagasse and acid for cassava peels) increased total carbohydrate contents to 85.4 ± 2.33 and 80.4 ± 2.5 for sugarcane bagasse and cassava peels, respectively. The respective lignin contents after pretreatment were 4.2 ± 0.44 and 4.8 ± 0.8 for sugarcane bagasse and cassava peels. Cultural conditions (pH, temperature, nitrogen source, inoculum size and substrate concentration) of the bacterium were optimized to enhance cellulase production. The laboratory scale fermentation of the feedstocks to ethanol was carried out in 250 mL Erlenmeyer flasks. Gas Chromatography -Mass spectrometry (GC-MS) analysis of the fermentation broth of sugarcane bagasse and cassava peels substrates revealed ethanol contents of 18.40 and 17.80 g/L, respectively. The study has demonstrated efficient bioethanol production by Bacillus cereus GBPS9 using sugarcane bagasse and cassava peels as feedstocks.

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“…Many researchers have exploited the extracellular enzyme production ability of various Bacillus spp. using submerged or solid state fermentation (Apun et al, 2000;Lee et al, 2008;Abdel-Mawgoud et al, 2008;Santhi, 2014). On this context, the present study was aimed to exploit B. licheniformis MTCC 429 for cellulase production ability using sugarcane bagasse as medium substrates.…”

Section: Introductionmentioning

confidence: 99%

Exploitation of Agro-Industrial Wastes as Substrates for Cellulase Production by Bacillus licheniformis MTCC 429

Kumaran¹,

Kalaichelv²,

Santhi³

2015

Microbiology J.

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The aim of the present investigation was to exploit the cheap agro-waste, sugarcane bagasse as a substrate for cellulase enzyme production using Bacillus licheniformis MTCC 429. The cellulase producing ability of B. licheniformis MTCC 429 was assessed using CMC agar plates. The factors affecting cellulase production were optimized by varying the parameters of incubation period, temperature and pH. The maximum cellulase enzyme production was achieved when the production medium pH was maintained at pH 7.0, temperature of 35°C with an incubation time of 48 h. Among the different concentration of sugarcane bagasse hydrolysate tested, CMC medium amended with 5% seemed to exhibit maximum cellulase productivity. Further, the partially purified enzyme was tested for its pH and temperature stability. The properties presented by B. licheniformis MTCC 429 suggest that the strain showed enhanced production of lignocellulosedegrading cellulase enzyme under the optimized conditions. The temperature and pH stability of the enzyme suggested its potential for industrial applications involving elevated conditions.

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Screening and isolation of a cellulolytic and amylolytic Bacillus from sago pith waste.

Cited by 83 publications

References 14 publications

culture conditions for production of cellulase by Aspergillus tubingensis KY615746 using rice straw waste

culture conditions for production of cellulase by Aspergillus tubingensis KY615746 using rice straw waste

Bioethanol Production by an Ethanol-Tolerant Bacillus cereus Strain GBPS9 Using Sugarcane Bagasse and Cassava Peels as Feedstocks

Exploitation of Agro-Industrial Wastes as Substrates for Cellulase Production by Bacillus licheniformis MTCC 429

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