Screening and In Vitro Evaluation of Mucoadhesive Thermoresponsive System Containing Methylene Blue for Local Photodynamic Therapy of Colorectal Cancer

Borghi-Pangoni, Fernanda Belincanta; Junqueira, Mariana Volpato; Ferreira, Sabrina Barbosa de Souza; Silva, Larissa Lachi; Rabello, Bruno Ribeiro; Caetano, Wilker; Diniz, Andréa; Bruschi, Marcos Luciano

doi:10.1007/s11095-015-1826-8

Cited by 31 publications

(30 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the natural clearance mechanisms from these sites limit residence time, decreasing drug absorption or duration of local effect [1]. In order to overcome these disadvantages, “mucoadhesive” systems have been developed, which adhere to mucosal membranes through a variety of attractive physicochemical interactions, enhancing retention, and thus the efficacy of medicines [2,3,4,5].…”

Section: Introductionmentioning

confidence: 99%

“…The detachment force method (also known as the tensile method) is the most widely employed method to investigate adhesive interactions between a mucosal membrane (or other substrate) and a formulation. This method can be used for solid [19,20,21] and semisolid dosage forms [2,4,5,12,19,21,22,23] and it is known that instrumental parameters and experiment design influence test results. Other techniques, such as the rheological method, can result in different responses and interpretations depending on the analysis type used (flow or oscillatory) [19].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Assessing Mucoadhesion in Polymer Gels: The Effect of Method Type and Instrument Variables

et al. 2018

View full text Add to dashboard Cite

Abstract:The process of mucoadhesion has been widely studied using a wide variety of methods, which are influenced by instrumental variables and experiment design, making the comparison between the results of different studies difficult. The aim of this work was to standardize the conditions of the detachment test and the rheological methods of mucoadhesion assessment for semisolids, and introduce a texture profile analysis (TPA) method. A factorial design was developed to suggest standard conditions for performing the detachment force method. To evaluate the method, binary polymeric systems were prepared containing poloxamer 407 and Carbopol 971P ® , Carbopol 974P ® , or Noveon ® Polycarbophil. The mucoadhesion of systems was evaluated, and the reproducibility of these measurements investigated. This detachment force method was demonstrated to be reproduceable, and gave different adhesion when mucin disk or ex vivo oral mucosa was used. The factorial design demonstrated that all evaluated parameters had an effect on measurements of mucoadhesive force, but the same was not observed for the work of adhesion. It was suggested that the work of adhesion is a more appropriate metric for evaluating mucoadhesion. Oscillatory rheology was more capable of investigating adhesive interactions than flow rheology. TPA method was demonstrated to be reproducible and can evaluate the adhesiveness interaction parameter. This investigation demonstrates the need for standardized methods to evaluate mucoadhesion and makes suggestions for a standard study design.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessing Mucoadhesion in Polymer Gels: The Effect of Method Type and Instrument Variables

et al. 2018

View full text Add to dashboard Cite

show abstract

“…[79][80][81] These stimuli exist in organisms, highlighting the possible suitability of such polymers for the development of controlled release and bioadhesive systems as they have stimuli-induced viscosity. [82][83][84][85] Mucoadhesion of thermosensitive gels and LCs can be explained by their rheological properties. 39,41,[86][87][88][89][90][91] Moreover, the mucoadhesive force of the liquid crystalline phases is determined by their ability to take up water from the environment.…”

mentioning

confidence: 99%

Design, characterization, and biological evaluation of curcumin-loaded surfactant-based systems for topical drug delivery

Fonseca-Santos

Santos

Rodero

et al. 2016

IJN

View full text Add to dashboard Cite

From previous studies, it has been found that curcumin exhibits an anti-inflammatory activity and is being used for the treatment of skin disorders; however, it is hydrophobic and has weak penetrating ability, resulting in poor drug transport through the stratum corneum. The aim of this study was to develop liquid crystalline systems for topical administration of curcumin for the treatment of inflammation. These liquid crystalline systems were developed from oleic acid, polyoxypropylene (5) polyoxyethylene (20) cetyl alcohol, and water as the surfactant, oil phase, and aqueous phase, respectively. These systems were characterized, and polarized light microscopy showed anisotropy with lamellar mesophases (Formulation 1) and hexagonal mesophases (Formulations 2 and 3), which were confirmed by the peak ratio measured using small-angle X-ray scattering. In addition, rheological tests revealed that the formulations exhibited gel-like behavior (G′>G″), as evidenced by the increased G′ values that indicate structured systems. Texture profile analysis showed that hexagonal mesophases have high values of hardness, adhesiveness, and compressibility, which indicate structured systems. In vitro studies on bioadhesion revealed that the hexagonal mesophases increased the bioadhesiveness of the systems to the skin of the pig ear. An in vivo inflammation experiment showed that the curcumin-loaded hexagonal mesophase exhibited an anti-inflammatory activity as compared to the positive control (dexamethasone). The results suggest that this system has a potential to be used as a bioadhesive vehicle for the topical administration of curcumin. Therefore, it is possible to conclude that these systems can be used for the optimization of drug delivery systems to the skin.

show abstract

“…Subsequently, 16 mL of the release media (Tween 80 aqueous solution; 1%, v/v) was carefully added in the recipient to obtain sink conditions and kept under constant agitation (Fig. 15) [11,56,101]. 500 µL aliquots of samples were collected and replaced with the same volume of fresh medium at fixed time intervals, 0.5 h, 1 h, 2 h, 3 h, 4 h, 6 h, 8 h and 24 h. These samples were diluted with methanol (1:2), filtered with PTFE membranes and quantified by the HPLC method, as previously described [30].…”

Section: Methodsmentioning

confidence: 99%

Design of a nanostructured mucoadhesive system containing curcumin for buccal application: from physicochemical to biological aspects

Ferreira¹,

Braga²,

Oliveira³

et al. 2019

Beilstein J. Nanotechnol.

Self Cite

View full text Add to dashboard Cite

Mucoadhesive nanostructured systems comprising poloxamer 407 and Carbopol 974P® have already demonstrated good mucoadhesion, as well as improved mechanical and rheological properties. Curcumin displays excellent biological activity, mainly in oral squamous cancer; however, its physicochemical characteristics hinder its application. Therefore, the aim of this study was to develop nanostructured formulations containing curcumin for oral cancer therapy. The photophysical interactions between curcumin and the formulations were elucidated by incorporation kinetics and location studies. They revealed that the drug was quickly incorporated and located in the hydrophobic portion of nanometer-sized polymeric micelles. Moreover, the systems displayed plastic behavior with rheopexy characteristics at 37 °C, viscoelastic properties and a gelation temperature of 36 °C, which ensures increased retention after application in the oral cavity. The mucoadhesion results confirmed the previous findings with the nanostructured systems showing a residence time of 20 min in porcine oral mucosa under flow system conditions. Curcumin was released after 8 h and could permeate through the porcine oral mucosa. Cytotoxicity testing revealed that the formulations were selective to cancer cells over healthy cells. Therefore, these systems could improve the physicochemical characteristics of curcumin by providing improved release and permeation, while selectivity targeting cancer cells.

show abstract

Screening and In Vitro Evaluation of Mucoadhesive Thermoresponsive System Containing Methylene Blue for Local Photodynamic Therapy of Colorectal Cancer

Abstract: The data obtained from the system composed of 20% Polox, 0.15% Carb, and 0.25% MB indicated a potentially functional role in PDT of the colorectal cancer and suggest it is worthy of clinical evaluation.

Cited by 31 publications

References 56 publications

Assessing Mucoadhesion in Polymer Gels: The Effect of Method Type and Instrument Variables

Assessing Mucoadhesion in Polymer Gels: The Effect of Method Type and Instrument Variables

Design, characterization, and biological evaluation of curcumin-loaded surfactant-based systems for topical drug delivery

Design of a nanostructured mucoadhesive system containing curcumin for buccal application: from physicochemical to biological aspects

Contact Info

Product

Resources

About