Screening and identification of novel isolate Streptomyces sp., NLKPB45 from Nellore costal region for its biomedical applications

Abstract

Actinobacteria, which are the prolific producers of antibiotics and significant suppliers to the pharmaceutical industry, can produce a wide variety of bioactive metabolites. An actinomycete strain designated NLKPB45 was isolated from mangrove soils samples of Nellore coastal regions Andhra Pradesh and assessed for antibiotic production and activity against pathogenic bacteria. From a total of 9 mangrove soil samples, 143 acinomycetes were isolated. Among the isolated them 6 actinomycetes strains showed potential antibacterial activity against at two tested pathogens gram positive and gram negative bacteria E. coli and S. aureus. The potent strain NLKPB45 was identified by 16S gene isolation and sequencing to the Streptomyces genus. The ethyl acetate extracts also as shown excellent antimicrobial activity against Salmonella sp., staphylococcus aureus, E. coli, and B. subtilus were detected in both the supernatant extract samples from fermentations of culture NLKPB45. The anticancer activity of extracts in the HeLa with IC50 value of 37.1924 μg/ml, MCF-7 IC50 value of 40.9177 μg/ml and HT 29 IC50 value of 43.3758 μg/ml.

Antimicrobial Potential of Streptomyces ansochromogenes (PB3) Isolated From a Plant Native to the Amazon Against Pseudomonas aeruginosa

“… Al-Ansari et al (2019) reported the isolation of ten actinomycetes from the soil, of which five demonstrated activity against Enterobacter aerogenes and Proteus mirabilis . Kalyani et al (2019) isolated 143 actinomycetes, including a Streptomyces sp. NLKPB-45 showed activity against Gram-positive bacteria, S. aureus , and Gram-negative Escherichia coli and P. aeruginosa .…”

“…against P. aeruginosa . Kalyani et al (2019) reported the activity of Streptomyces sp. NLKPB-45 against Gram-negative bacteria Escherichia coli and P. aeruginosa .…”

Current strategic limitations of phylogenetic tools badly impact the inference of an evolutionary tree

“…The study showcases a strategy to circumvent the potential pitfalls of the evolutionary algorithm. It will allow us consistently construct an evolutionary correct model for a sequence dataset and will subsequently improve the prediction accuracy of several different types of biological studies, be it a sequence characterization (Guder and Krishna 2019;Kalyani et al 2019;Mallu et al 2019;Satyanarayana et al 2017;Somavarapu et al 2017;Vemuri et al 2019) or proteomic study (Jaswanthi et al 2019;Rao et al 2017;Samara Shekar Reddy et al 2019;Srideepthi et al 2017).…”

Excavating the functionally crucial active-site residues of the DXS protein of Bacillus subtilis by exploring its closest homologues

“…It would also allow in extracting the evolutionarily closest templates for the protein sequences and would aid to improve the accuracy of the conventional template-based protein modeling protocols [82][83][84][85][86][87][88][89][90]. The strategy would be significantly useful for improving the algorithmic accuracy of several related research works including protein modeling [91] and folding [92] and functional enzymatic characterization [93][94][95][96][97][98][99][100].…”