Screening and identification of newly isolated cellulose-degrading bacteria from the gut of xylophagous termite Microcerotermes diversus (Silvestri)

Pourramezan, Zahra; Ghezelbash, Gholam Reza; Romani, Bizhan; Ziaei, Saeid; Hedayatkhah, Abolghasem

doi:10.1134/s0026261712060124

Cited by 35 publications

(30 citation statements)

References 22 publications

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“…It has been reported that fungi are potent cellulase and hemicellulase producers, and that, among fungal species, Aspergillus spp. notably produces copious amounts of such enzymes (Li et al 2014, 2015b; Pourramezan et al 2012). In the current study, the CS-1 strain exhibited a better cellulose-degrading activity (Table 2) than other strains (Li et al 2003; Sachslehner et al 1997; Pourramezan et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…notably produces copious amounts of such enzymes (Li et al 2014, 2015b; Pourramezan et al 2012). In the current study, the CS-1 strain exhibited a better cellulose-degrading activity (Table 2) than other strains (Li et al 2003; Sachslehner et al 1997; Pourramezan et al 2012). It is worth noting that the carbon source in the growth medium considerably affects the synthesis of cellulolytic enzymes in liquid culture, with the highest amounts of cellulases produced by A. niger with sodium carboxymethylcellulose as a carbon source (Gautam et al 2010).…”

Section: Discussionmentioning

confidence: 99%

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Isolation and characterization of phosphofungi, and screening of their plant growth-promoting activities

Wang

Sui

et al. 2018

AMB Expr

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Rhizospheric microorganisms can increase phosphorus availability in the soil. In this regard, the ability of phosphofungi to dissolve insoluble phosphorus compounds is greater than that of phosphate-solubilizing bacteria. The aim of the current study was to identify efficient phosphofungi that could be developed as commercial microbial agents. Among several phosphate-solubilizing fungal isolates screened, strain CS-1 showed the highest phosphorus-solubilization ability. Based on phylogenetic analysis of the internal transcribed spacer region sequence, it was identified as Aspergillus niger. High-performance liquid chromatography analysis revealed that the mechanism of phosphorus solubilization by CS-1 involved the synthesis and secretion of organic acids, mainly oxalic, tartaric, and citric acids. Furthermore, strain CS-1 exhibited other growth-promoting abilities, including efficient potassium release and degradation of crop straw cellulose. These properties help to returning crop residues to the soil, thereby increasing nutrient availability and sustaining organic matter concentration therein. A pot experiment revealed that CS-1 apparently increased the assessed biometric parameters of wheat seedlings, implying the potential of this strain to be developed as a commercial microbial agent. We used Illumina MiSeq sequencing to investigate the microbial community composition in the rhizosphere of uninoculated wheat plants and wheat plants inoculated with the CS-1 strain to obtain insight into the effect of the CS-1 strain inoculation. The data clearly demonstrated that CS-1 significantly reduced the content of pathogenic fungi, including Gibberella, Fusarium, Monographella, Bipolaris, and Volutella, which cause soil-borne diseases in various crops. Strain CS-1 may hence be developed into a microbial agent for plant growth improvement.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0593-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of phosphofungi, and screening of their plant growth-promoting activities

Wang

Sui

et al. 2018

AMB Expr

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“…The genus Acinetobacter was present in the gut of the termite Cortaritermes fulviceps (Ben Guerrero et al, 2015), in the midgut and haemolymph of Leptinotarsa decemlineata , Microcerotermes diversus , and a in the gut of the giant African snail ( Archachatina marginata ). Their cellulase, xylanase and β-glucosidase activities were quantitatively evaluated (Ekperigin, 2007; Pourramezan et al, 2012; Vilanova et al, 2012). Delftia sp.…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Lignocellulosic Diets on Bacterial Microbiota and Hydrolytic Enzyme Activities in the Gut of the Cotton Boll Weevil (Anthonomus grandis)

et al. 2016

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Cotton boll weevils, Anthonomus grandis, are omnivorous coleopteran that can feed on diets with different compositions, including recalcitrant lignocellulosic materials. We characterized the changes in the prokaryotic community structure and the hydrolytic activities of A. grandis larvae fed on different lignocellulosic diets. A. grandis larvae were fed on three different artificial diets: cottonseed meal (CM), Napier grass (NG) and corn stover (CS). Total DNA was extracted from the gut samples for amplification and sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. Proteobacteria and Firmicutes dominated the gut microbiota followed by Actinobacteria, Spirochaetes and a small number of unclassified phyla in CM and NG microbiomes. In the CS feeding group, members of Spirochaetes were the most prevalent, followed by Proteobacteria and Firmicutes. Bray–Curtis distances showed that the samples from the CS community were clearly separated from those samples of the CM and NG diets. Gut extracts from all three diets exhibited endoglucanase, xylanase, β-glucosidase and pectinase activities. These activities were significantly affected by pH and temperature across different diets. We observed that the larvae reared on a CM showed significantly higher activities than larvae reared on NG and CS. We demonstrated that the intestinal bacterial community structure varies depending on diet composition. Diets with more variable and complex compositions, such as CS, showed higher bacterial diversity and richness than the two other diets. In spite of the detected changes in composition and diversity, we identified a core microbiome shared between the three different lignocellulosic diets. These results suggest that feeding with diets of different lignocellulosic composition could be a viable strategy to discover variants of hemicellulose and cellulose breakdown systems.

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“…The bodies of termites were ground and serially diluted with Milli Q water. The dilute sample was spread over nutrient agar media with 1% carboxymethyl cellulose (CMC) and 1% beechwood xylan provided by M. S. Traders (Sigma, Lahore, Pakistan) (Dheeran et al 2012;Pourramezan et al 2012). The plates were incubated at 30 C for 24 h.…”

Section: Isolation and Screening Of Bacteriamentioning

confidence: 99%

Cellulomonas sp. Isolated from Termite Gut for Saccharification and Fermentation of Agricultural Biomass

et al. 2017

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Biofuel is an important alternative source of fuel, as many countries are looking to decrease their dependence on fossil fuels. One of the critical steps in biofuel production is the conversion of lignocelluloses to fermentable sugars, and there is need for cheaper and more efficient enzymatic strategies. Consequently, lignocellulase genes from various organisms have been explored. Termites possess varied sets of efficient micro-scale lignocellulose degrading systems. In this study, bacteria that degraded cellulose and xylan were isolated from termite gastrointestinal tract. The isolate was identified as Cellulomonas sp. by 16S rRNA gene sequencing. The bacterial enzymes cellulase and xylanase showed the highest activity at 50 °C and pH 8.0. The agricultural substrates were hydrolyzed by cellulases and xylanases, and more sugar was released from corn stover (18.903+0.65 mM/L) than from rice straw or cotton stalk. After direct hydrolysis and fermentation of agricultural substrates, ethanol (0.425+0.035 g/L) and lactate (0.772+0.075 g/L) were the major end products. Thus, termite gut bacteria can efficiently hydrolyze hemicellulose and cellulose, and these bacteria also have the potential to convert these fermentable sugars into valuable secondary metabolites.

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Screening and identification of newly isolated cellulose-degrading bacteria from the gut of xylophagous termite Microcerotermes diversus (Silvestri)

Cited by 35 publications

References 22 publications

Isolation and characterization of phosphofungi, and screening of their plant growth-promoting activities

Isolation and characterization of phosphofungi, and screening of their plant growth-promoting activities

Effect of Different Lignocellulosic Diets on Bacterial Microbiota and Hydrolytic Enzyme Activities in the Gut of the Cotton Boll Weevil (Anthonomus grandis)

Cellulomonas sp. Isolated from Termite Gut for Saccharification and Fermentation of Agricultural Biomass

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