Screening and Identification of Mannanase-Producing Fungi Isolated from Selected Agricultural Wastes

Arotupin, D. J.; Olaniyi, Oladipo Oladiti

doi:10.9734/bmrj/2013/4218

Cited by 17 publications

(24 citation statements)

References 10 publications

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“…and Scopulariopsis candida. The secretion of mannanases by these isolates on LBG media could be attributed to the ability of their genetic make up to secrete active mannanase coupled with varied diffusion rate [10]. All the tested fungal strains were able to produce extracellular mannanase in submerged state fermentation, although with differences in the rate of enzyme production.…”

Section: Discussionmentioning

confidence: 99%

“…The excessive addition of organic wastes (nutrients) to water bodies is known to stimulate excessive growth of microorganisms [10].…”

Section: Discussionmentioning

confidence: 99%

“…The samples were transferred to the laboratory and were used as sources for the isolation of mannanase-producing fungi [9,10].…”

Section: Sources Of Samplementioning

confidence: 99%

“…Medium composition described by Mandles & Weber [13] modified by Arotupin & Olaniyi [10] was used for submerged fermentation (static condition). The basal medium contained (g/L): LBG 10g, peptone 2g, yeast extract 2g, NaNO 3 2 g, KH 2 PO 4 1 g, MgSO 4 .7H 2 O 0.5 g, KCl 0.5 g and FeSO 4 .7H 2 O traces.…”

Section: Screening Of Mannanase-producing Fungi In Submerged State Fementioning

confidence: 99%

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2017

JBMOA

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The present study aimed at the isolation, screening and partial purification of beta-mannanase from fungi isolated from soil and water samples collected from Ilaje Lake, Ondo state, Nigeria. The associated fungi were isolated and counted by standard microbiological methods. Partial purification of crude mannanase was done by standard biochemical methods. Quantitatively, mannanase production was performed in mineral salt medium into which Locust Bean Gum (LBG) had been incorporated as the sole carbon source. Enzyme activity was determined by dinitrosalicylic acid (DNSA) method, while protein content was evaluated by Lowry's method. The highest fungal counts were recorded for water sample collected from Ilaje Lake with 3.4×10 8 sfu/mL. The organisms encountered include Aspergillus flavus, Rhizopus stolonifer, A. fumigatus, A. niger, R. japonicus, Penicillum italicum, Fusarium solani and Candida albicans. All the fungal isolates encountered from these sources showed varied degrees of mannanase activities. The highest specific mannanase activity was recorded for isolate 4B1, while the lowest value was obtained with isolate 1B2. Purification of crude mannanase from A. niger was carried out by ammonium sulphate precipitation and gel filtration . Fractionation of ammonium sulphate precipitated mannanase from A. niger on Sephadex G-200 produced two activity peaks. In this investigation, fungal isolates evaluated for mannanase production from this source gave appreciable mannanase activity and this could be applied in many industrial processes. Keywords: Materials and Methods Sources of SampleWater and soil samples were collected from Ilaje Lake, Ondo State, Nigeria in sterile bottles. The samples were transferred to the laboratory and were used as sources for the isolation of mannanase-producing fungi [9,10]. Isolation and identification of mannanase-producing fungi from soil and water samplesThe total fungal counts from the samples were determined using the pour plate method on potato dextrose agar. The samples were serially diluted and 1ml of an appropriate dilution was used to inoculate the plate in duplicate. The plates were incubated at 28 ± 2˚C for 72hrs, after which the total colony count was determined as previously described [11]. At the end of incubation, the colonies were sub-cultured from the mixed cultures and identified on the bases of cultural characters (colour, shape of colony, surface and reverse pigmentation and texture of the colony) as well as microscopic structure (septate or nonseptate hyphae, structure of hyphae and conidia) [12]. Screening of mannanase-producing fungi in submerged state fermentationMedium composition described by Mandles & Weber [13] modified by Arotupin & Olaniyi [10] was used for submerged fermentation (static condition). The basal medium contained (g/L): LBG 10g, peptone 2g, yeast extract 2g, NaNO 3 2 g, KH 2 PO 4 1 g, MgSO 4 .7H 2 O 0.5 g, KCl 0.5 g and FeSO 4 .7H 2 O traces. The pH of the media was adjusted to 6.8 with pH meter (Denver Instrument, Model 20 pH/ Co...

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Section: Discussionmentioning

confidence: 99%

“…The excessive addition of organic wastes (nutrients) to water bodies is known to stimulate excessive growth of microorganisms [10].…”

Section: Discussionmentioning

confidence: 99%

“…The samples were transferred to the laboratory and were used as sources for the isolation of mannanase-producing fungi [9,10].…”

Section: Sources Of Samplementioning

confidence: 99%

Section: Screening Of Mannanase-producing Fungi In Submerged State Fementioning

confidence: 99%

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Untitled

2017

JBMOA

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“…The strain whose origin was yam peels, screened and confirmed to be positive for mannanase activity by plate assay technique was used in this study (Arotupin and Olaniyi, 2013). This strain was selected based on its performance in terms of quantitative mannanase production both in solid and submerged state fermentation.…”

Section: Methodsmentioning

confidence: 99%

Chemical Mutagenesis of Penicillium italicum for the Development of Catabolite Insensitive Mutants

Olaniyi¹,

Ibitoye²,

Osunla³

2015

Microbiology J.

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The objective of this study was to enhance mannanase production through chemical mutagenesis and to develop catabolite insensitive mutants. Mutants were generated by incubating spore suspension of Penicillium italicum with varying concentrations of Ethyl Methane Sulfonate (EMS). Wild type and mutants were screened for mannanase production in basal media supplemented with Locust Bean Gum (LBG) as an inducer. Mannanase activity was determined by dinitrosalicylic acid method, while protein content was determined by Lowry method. Approximately 46% of the mutants generated showed higher mannanase activity in comparison with the wild type, while repression of enzyme biosynthesis was observed in others. The isolated mutants were screened for catabolite activation studies in the presence of different mannose concentrations (0.1, 0.5 and 1% w/v) as a carbon source. The supplementation of 0.1% (w/v) mannose in the fermentation media caused enhancement of mannanase synthesis in approximately 54% of the mutants. The supplementation of 0.5 and 1% (w/v) mannose in the fermentation media caused improvement of mannanase biosynthesis in 100% and approximately 62% of the mutants, respectively. The results indicated that EMS might be an effective mutagenic agent for the development of catabolite insensitive mutants.

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