Screening and identification of lipase inhibitors extracted from Dioscorea nipponica Makino by UV–vis and HPLC coupled to UPLC-Q-TOF-MS/MS

Jin, Peiyi; Chen, Linzhou; Zhong, Jinjian; Yuan, Tiefeng; Gan, Lu; Huang, Jilong; Wang, Liping; Fan, Honghai; Chen, Lin

doi:10.1016/j.ijbiomac.2023.123427

Cited by 4 publications

(2 citation statements)

References 46 publications

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“…spongiosa . Herein, three different resins (ADS‐7 [13], D‐101 [26], and AB‐8 [27]) were investigated. The crude extracts of D .…”

Section: Resultsmentioning

confidence: 99%

“…Pretreatment by MRCC was carried out to enrich the targets from D. spongiosa. Herein, three different resins (ADS-7 [13], D-101 [26], and AB-8 [27]) were investigated. The crude extracts of D. spongiosa were added to the above three resins, which were further eluted using water, 20%, 40%, 60%, 80%, and 100% ethanol.…”

Section: Optimization Of Mrcc For the Crude Extract Of D Spongiosamentioning

confidence: 99%

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Efficient discovery of active isolates from Dioscorea spongiosa by the combination of bioassay‐guided macroporous resin column chromatography and high‐speed counter‐current chromatography

Wang,

Yu,

Zhao

et al. 2024

J of Separation Science

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In the present study, twelve compounds from Dioscorea spongiosa were successfully purified by an efficient technique combined bioassay‐guided fractionation macroporous resin column chromatography (MRCC) pretreatment and high‐speed counter‐current chromatography (HSCCC) separation for the first time. Then, D101 MRCC was used to fractionate the crude extract into five parts, which further applied the bioassay‐guided fractionation strategy to screen the active fractions of 2 and 4. As for the separation, 200 mg Fr.2 was purified by HSCCC using EtOAc/n‐BuOH/H2O (2:2:3, v/v), leading to annulatomarin (1), dioscoresides C (2), diosniponol C (3), methyl protodioscin (4), pseudoprotodioscin (5), protogracillin (6), as well as 200 mg Fr.4 yielding montroumarin (7), dioscorone A (8), diosniponol D (9), protodioscin (10), gracillin (11), and dioscin (12) using CH2Cl2/MeOH/H2O (3:3:2, v/v) with the purities over 95.0%. Finally, the isolates were assayed for their anti‐inflammatory, urico‐lowering, and anti‐diabetic activities in vitro, which indicated that the steroidal saponins of 5, 6, and 11 showed all these three activities.

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“…spongiosa . Herein, three different resins (ADS‐7 [13], D‐101 [26], and AB‐8 [27]) were investigated. The crude extracts of D .…”

Section: Resultsmentioning

confidence: 99%

Section: Optimization Of Mrcc For the Crude Extract Of D Spongiosamentioning

confidence: 99%

Efficient discovery of active isolates from Dioscorea spongiosa by the combination of bioassay‐guided macroporous resin column chromatography and high‐speed counter‐current chromatography

Wang,

Yu,

Zhao

et al. 2024

J of Separation Science

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show abstract

Dioscorea nipponica Makino: A comprehensive review of its chemical composition and pharmacology on chronic kidney disease

Wu,

Zhang,

Wang

et al. 2023

Biomedicine & Pharmacotherapy

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Establishment and application of a screening method for α-glucosidase inhibitors based on dual sensing and affinity chromatography

Zhang,

Wang,

Wang

et al. 2024

Journal of Chromatography A

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Screening and identification of lipase inhibitors extracted from Dioscorea nipponica Makino by UV–vis and HPLC coupled to UPLC-Q-TOF-MS/MS

Cited by 4 publications

References 46 publications

Efficient discovery of active isolates from Dioscorea spongiosa by the combination of bioassay‐guided macroporous resin column chromatography and high‐speed counter‐current chromatography

Efficient discovery of active isolates from Dioscorea spongiosa by the combination of bioassay‐guided macroporous resin column chromatography and high‐speed counter‐current chromatography

Dioscorea nipponica Makino: A comprehensive review of its chemical composition and pharmacology on chronic kidney disease

Establishment and application of a screening method for α-glucosidase inhibitors based on dual sensing and affinity chromatography

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