Screening and identification of key biomarkers in bladder carcinoma: Evidence from bioinformatics analysis

Abstract

Bladder cancer (BC) is one of the most common urogenital malignancies. However, present studies of its multiple gene interaction and cellular pathways remain unable to accurately verify the genesis and the development of BC. The aim of the present study was to investigate the genetic signatures of BC and identify its potential molecular mechanisms. The gene expression profiles of GSE31189 were downloaded from the Gene Expression Omnibus database. The GSE31189 dataset contained 92 samples, including 52 BC and 40 non-cancerous urothelial cells. To further examine the biological functions of the identified differentially expressed genes (DEGs), Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses were performed, and a protein-protein interaction (PPI) network was mapped using Cytoscape software. In total, 976 DEGs were identified in BC, including 457 upregulated genes and 519 downregulated genes. GO and KEGG pathway enrichment analyses indicated that upregulated genes were significantly enriched in the cell cycle and the negative regulation of the apoptotic process, while the downregulated genes were mainly involved in cell proliferation, cell adhesion molecules and oxidative phosphorylation pathways (P<0.05). From the PPI network, the 12 nodes with the highest degrees were screened as hub genes; these genes were involved in certain pathways, including the chemokine-mediated signaling pathway, fever generation, inflammatory response and the immune response nucleotide oligomerization domain-like receptor signaling pathway. The present study used bioinformatics analysis of gene profile datasets and identified potential therapeutic targets for BC.

Association of Mps one binder kinase activator 1 (MOB1) expression with poor disease‐free survival in individuals with non‐small cell lung cancer

“…Among the genes found to be greatly elevated, only a few studies have been conducted concerning PGM5 in cancer 21, 22 and its function has remained undetermined. MCAM (also known as CD146, MUC18 or MelCAM) and ADAM12 have been known for its function in epithelial‐mesenchymal transition (EMT) 23–26 .…”

Systematic Analysis of the Lysine Malonylome in Sanghuangporus Sanghuang

“…Abundance analysis of KEGG pathways and analysis of protein domains with a p value < 0.05 were deemed signi cant. [33,34].…”

Assessment of molecular mechanisms and potential biomarkers in bladder urothelial carcinoma

“…Upregulated genes mainly annotated to cell tumor and control samples to reveal altered gene expression mechanisms of bladder urothelial carcinoma [5,6]. The increased expression of genes in bladder cancer found to be annotated to negative regulation apoptotic process, salmonella infection, amoebiasis, malaria pathways and rheumatoid arthritis pathways, while downregulated genes were annotated to oxidative phosphorylation, taurine and hypotaurine metabolism, vitamin digestion and absorption, arthritis and vibrio cholerae infection pathways [7]. Enrichment of upregulated genes in cell division and protein binding, enrichment of downregulated genes in extracellular matrix organization and complement and coagulation cascades was also reported [5].…”