Screening and identification of host proteins interacting with Toxoplasma gondii SAG2 by yeast two-hybrid assay

Lai, Meng Yee; Lau, Yee Ling

doi:10.1186/s13071-017-2387-y

Cited by 9 publications

(3 citation statements)

References 30 publications

(31 reference statements)

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“…The results obtained in the different research works reporting on the pathogenesis, immunogenicity, and treatment of T. gondii are worthy to be contextualized. The articles relative to Malaysia indicated some suitable proteins markers that can serve either for treatment or as good candidates for vaccine development [ 70 , 71 , 72 , 77 , 83 , 84 ]. Additionally, the fact that some of the drugs for the treatment of T. gondii are teratogenic and still no cure for the bradyzoite tissue cysts must be taken into consideration.…”

Section: Innovative Diagnostic Approach and Vaccine Development Fomentioning

confidence: 99%

A Review on the Prevalence of Toxoplasma gondii in Humans and Animals Reported in Malaysia from 2008–2018

Wana

Moklas

Watanabe

et al. 2020

IJERPH

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Toxoplasmosis is a disease caused by the protozoan parasite Toxoplasma gondii (T. gondii). Human toxoplasmosis seroprevalence in Malaysia has increased since it was first reported in 1973 as shown in previous reviews of 1991 and 2007. However, over a decade since the last review, comprehensive data on toxoplasmosis in Malaysia is lacking. This work aimed at reviewing articles on toxoplasmosis research in Malaysia in order to identify the research gaps, create public awareness, and efforts made so far and proffer management options on the disease. The present review examines the available published research articles from 2008 to 2018 related to toxoplasmosis research conducted in Malaysia. The articles reviewed were retrieved from nine credible databases such as Web of Science, Google Scholar, ScienceDirect, PubMed, Scopus, Springer, Wiley online library, Ovid, and Cochrane using the keywords; Malaysia, toxoplasmosis, Toxoplasma gondii, toxoplasma encephalitis, seroprevalence, human immunodeficiency virus (HIV) patients, pregnant women, genotype strain, anti-toxoplasma antibodies, felines, and vaccine. The data highlighted seropositive cases from healthy community members in Pangkor Island (59.7%) and among migrant workers (57.4%) at alarming rates, as well as 42.5% in pregnant women. Data on animal seroprevalence were limited and there was no information on cats as the definitive host. Genetic characterization of Toxoplasma gondii from HIV patients; pregnant women, and domestic cats is lacking. This present review on toxoplasmosis is beneficial to researchers, health workers, animal health professionals, and policymakers. Therefore, attention is required to educate and enlighten health workers and the general public about the risk factors associated with T. gondii infection in Malaysia.

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Section: Innovative Diagnostic Approach and Vaccine Development Fomentioning

confidence: 99%

A Review on the Prevalence of Toxoplasma gondii in Humans and Animals Reported in Malaysia from 2008–2018

Wana

Moklas

Watanabe

et al. 2020

IJERPH

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“…The full length CtBSL (1006 bp), CtJAZ3 (1241 bp) and CtNINJA (1361 bp), CtMYC2 (1062 bp) gene sequences were cloned in pGBKT7 (bait) and pGADT7 (prey) plasmids, respectively, and subsequently transformed into yeast two hybrid GOLD strain (Clontech), using a Frozen-EZ Yeast Transformation II Kit (Zymo Research). The protein interactions were analyzed on SD medium containing X-α-Gal (40 ng/mL) and Aureobasidin A (125 ng/mL), using quadruple dropout method (Lai and Lau, 2017). The list of primers used in this study is given in Table S1.…”

Section: Yeast Two Hybrid Assaymentioning

confidence: 99%

Increased Seed Size Mediated by Cyamopsis Tetragonoloba Big SEEDS LIKE Silencing Is Positively Co-related to Galactomannan Content, Phytochemical Biosynthesis and Anti-diabetic Potential of Guar

Thakur

Prasad

2021

Preprint

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In higher plants, seed size is an important agronomic trait that determines the crop yield and evolutionary fitness. Guar is an economically important legume crop because of the presence of galactomannan in its seed endosperm which is used as a stabilizer and thickener in pharmaceutical, paper, cosmetic and textile industries. The current study demonstrate the role of CtBSL, in regulation of seed size, weight and galactomannan content in two independent BSL12-25-15-31-3 and BSL70-21-25-4-16 T4 transgenic lines of guar, via. pCMKCtBSL mediated post transcriptional CtBSL silencing approach. The upregulation of CtCYCA2;4, CtCYCD3;2, CtCDKE-1, CtCYCT1;5, CtH2A, CtH2B, CtH3, CtH4, CtERF, CtWRKY43, CtGRF5 and CtGIF1 genes in transgenic lines of guar contributed to enhanced cell proliferation and final seed size which was further strongly and positively correlated to phytochemical biosynthesis and their anti-diabetic, anti-AGEs and lipase inhibitory potential against α-glucosidase, α-amylase, AGEs and lipase enzymes, respectively. Therefore, here we conclude the potential role of CtBSL silencing in improving the crop yield and therapeutic treatment of diabetes and obesity by positive regulation of seed size and phytochemical biosynthesis, respectively, in guar.

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“…The pGBKT7-evpP plasmid and empty pGBKT7 vector were transformed into the yeast strain Y2HGold using theYeast maker TM Yeast Transformation System 2 kit according to the manufacturer's manual (Clontech, Japan), respectively. Total proteins were detected using c-myc tag mouse McAb (Tianjin Saierbio, China) to detect evpP expression as described previously (Lai and Lau, 2017). The culture with recombinant pGBKT7-evpP bait were plated on SD/-Trp, SD/-Trp/X and SD/-Trp/X/A plates.…”

Section: Bait Plasmid Expression Auto-activation and Toxicity Testsmentioning

confidence: 99%

Roles of EvpP in Edwardsiella piscicida-Macrophage Interactions

Qin¹,

Wang²,

Gao³

et al. 2020

Front. Cell. Infect. Microbiol.

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Edwardsiella piscicida is found to be an important facultative intracellular pathogen with a broad host range. These organisms can replicate and survive within host macrophages to escape from the subversion of the immune defense. E. piscicida-macrophage interaction is very important in determining the outcome of edwardsiellasis. As an effector protein of E. piscicida T6SS, EvpP has been determined to be a very important virulence factor for E. piscicida, although its precise role in E. piscicida-macrophage interactions is not yet clear. In this study, the roles of EvpP in E. piscicida-macrophage interactions were characterized. Here, we constructed the deletion mutants of evpP (evpP) and complementation (evpP-C) by the allelic exchange method. Compared to wild type strain (WT), evpP was found to be attenuated for growth within macrophages. In line with this observation, we found its survival capacity was lower than WT under oxidative and acid stress in vitro, which simulate conditions encountered in host macrophages. Attenuation of evpP also correlated with enhanced activation of macrophages, as reflected by augmented NO production in evpP-treated macrophages. Moreover, compared to WT, evpP induced markedly increased apoptosis of macrophages, characterized by increased Annexin V binding and the activation of cleaved caspase-3. These findings provided strong evidence that EvpP is involved in the process of E. piscicida-macrophage interactions and is required for its survival and replication in macrophages. Thus, we propose that EvpP might be an important factor that controlling the fate of E. piscicida inside macrophages. To further exploring the underlying mechanism of EvpP action, the cDNA library was constructed from E. piscicida-infected macrophages and a yeast two-hybrid screen was performed to search for cellular proteins interacting with EvpP. Ribosomal protein S5 (RPS5) was identified as a target of EvpP. Furthermore, the interaction was validated with co-immunoprecipitation assay. This result implies that the observed effect of EvpP on macrophages might be related to RPS5-mediated regulation, contributing to a better understanding of the mechanisms of EvpP involved in E. piscicida-macrophage interactions.

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Screening and identification of host proteins interacting with Toxoplasma gondii SAG2 by yeast two-hybrid assay

Cited by 9 publications

References 30 publications

A Review on the Prevalence of Toxoplasma gondii in Humans and Animals Reported in Malaysia from 2008–2018

A Review on the Prevalence of Toxoplasma gondii in Humans and Animals Reported in Malaysia from 2008–2018

Increased Seed Size Mediated by Cyamopsis Tetragonoloba Big SEEDS LIKE Silencing Is Positively Co-related to Galactomannan Content, Phytochemical Biosynthesis and Anti-diabetic Potential of Guar

Roles of EvpP in Edwardsiella piscicida-Macrophage Interactions

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