Screening and identification of five serum proteins as novel potential biomarkers for cured pulmonary tuberculosis

Abstract

Rapid and efficient methods for the determination of cured tuberculosis (TB) are lacking. A total of 85 differentially expressed serum proteins were identified by iTRAQ labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) analysis (fold change >1.50 or <0.60, P < 0.05). We validated albumin (ALB), Rho GDP-dissociation inhibitor 2 (ARHGDIB), complement 3 (C3), ficolin-2 (FCN2), and apolipoprotein (a) (LPA) using the enzyme-linked immunosorbent assay (ELISA) method. Significantly increased ALB and LPA levels (P = 0.036 and P = 0.012, respectively) and significantly reduced ARHGDIB, C3, and FCN2 levels (P < 0.001, P = 0.035, and P = 0.018, respectively) were observed in cured TB patients compared with untreated TB patients. In addition, changes in ALB and FCN2 levels occurred after 2 months of treatment (P < 0.001 and P = 0.030, respectively). We established a cured TB model with 87.10% sensitivity, 79.49% specificity, and an area under the curve (AUC) of 0.876. The results indicated that ALB, ARHGDIB, C3, FCN2, and LPA levels might serve as potential biomarkers for cured TB. Our study provides experimental data for establishing objective indicators of cured TB and also proposes potential markers for evaluating the efficacy of anti-TB drugs.

Serum soluble VSIG4 as a surrogate marker for the diagnosis of lymphoma‐associated hemophagocytic lymphohistiocytosis

“…Eight‐plex isobaric tags for relative and absolute quantitation (iTRAQ) analysis was performed in the State Key Laboratory of Proteomics (Beijing Proteome Research Center, National Center for Protein Sciences Beijing). Sample preparation, mass spectrometry, peptide and protein identification were performed as previously described (Wang et al ., ; Sun et al ., ; Zhang et al ., ). All reported data were recorded using Excel for evaluation of candidate proteins based on proteomic fold‐change and false discovery rate (FDR).…”

iTRAQ‐Based Proteomics of Chronic Renal Failure Rats after FuShengong Decoction Treatment Reveals Haptoglobin and Alpha‐1‐Antitrypsin as Potential Biomarkers

“…To screen the differential proteins, the threshold was applied as follows: the unused ProtScore > 1.3 and at least one peptide with a 95% confidence level [42], ratios with fold change > 1.2 (or <0.83), and P values < 0.05 were considered to be significant.…”

Analysis of the plasma proteome using iTRAQ and TMT‐based Isobaric labeling

“…Bacterial studies include iTRAQ studies of pulmonary tuberculosis (PTB) in humans and mycobacterial infections in cattle . In the works of Xu et al and Wang et al, differences in serum proteome were studied in association with PTB . Included in these was the use of a pooled iTRAQ strategy to compare treated, untreated and cured PTB patients with healthy controls (250 samples in total), in which markers of cured PTB were identified .…”

“…In the works of Xu et al and Wang et al, differences in serum proteome were studied in association with PTB . Included in these was the use of a pooled iTRAQ strategy to compare treated, untreated and cured PTB patients with healthy controls (250 samples in total), in which markers of cured PTB were identified . While the latter studies were performed using ESI (AB Sciex 5600), Li et al used MALDI‐TOF/TOF (AB Sciex 5800) and iTRAQ to profile the serum of PTB patients, comparing sera from drug resistant, smear‐positive and smear‐negative pulmonary tuberculosis cases with normal controls and pneumonia patients (87 samples in iTRAQ discovery and 207 in the ELISA validations) and found that sex hormone binding globulin (SHBG) was significantly elevated in PTB .…”

“…45 In the works of Xu et al and Wang et al, differences in serum proteome were studied in association with PTB. 137,138,171 Included in these was the use of a pooled iTRAQ strategy to compare treated, untreated and cured PTB patients with healthy controls (250 samples in total), in which markers of cured PTB were identified. 171 While the latter studies were performed using ESI 139 Cao and co-workers used iTRAQ labeling to analyze plasma from sepsis patients, including ten 4-plex iTRAQ comparisons of infected and uninfected subjects (n = 39 in total).…”